Rivista
/
Biologia
/
Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

DOI:
10.3791/64614-v

•

07:35 min

•

October 06, 2022

•

, , ,
1Depto. Biología Celular, Genética y Fisiología, Instituto de Hortofruticultura Subtropical y Mediterránea, Campus de Teatinos,Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC)

Capitoli

  • 00:05Introduction
  • 00:54Plant Preparation
  • 02:14Inoculation of Arabidopsis and Bean Plants
  • 03:53Extraction of Bacteria from the Apoplast
  • 04:55Single-Cell Analysis of the Apoplast-Extracted Bacteria
  • 06:14Results: Analysis of the Apoplast-Extracted Bacteria Obtained from Arabidopsis or Bean Leaves After Bacterial Inoculation
  • 06:57Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol describes a method that allows single-cell gene expression analysis on Pseudomonas syringae populations grown within the plant apoplast.

Tags

Single-cell AnalysisPseudomonas SyringaeApoplastPlant InoculationBacterial ExtractionFlow CytometryPlant Pathogenic BacteriaBeneficial Endocytic BacteriaArabidopsisPhaseolus VulgarisLB PlateOptical Density

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

Single Cell Electroporation in vivo within the Intact Developing Brain

Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

In vitro Reconstitution of the Active T. castaneum Telomerase

Expression Analysis of Mammalian Linker-histone Subtypes

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

Visualizing Stromule Frequency with Fluorescence Microscopy

Read Article