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Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Article DOI: 10.3791/64614-v • 07:35 min • October 6th, 2022
October 6th, 2022 •
*1, *1, 1, 1
1Depto. Biología Celular, Genética y Fisiología, Instituto de Hortofruticultura Subtropical y Mediterránea, Campus de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC)
* These authors contributed equally

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The present protocol describes a method that allows single-cell gene expression analysis on Pseudomonas syringae populations grown within the plant apoplast.

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Single-cell Analysis Gene Expression Pseudomonas Syringae Plant Tissue Gentle Plant Inoculation Extraction Method Bacterial Populations Leaf Apoplast Single-cell Gene Expression Analysis Flow Cytometry Apoplastic Bacteria Recovery Plant Pathogenic Bacteria Endocytic Bacteria Nieves Lopez-Pagan Ph.D Student Pot Vermiculite Plant Substrate Mix Metal Mesh Arabidopsis Seeds Plastic Dome Relative Humidity Stratification Plant Growth Chamber
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