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Developmental Biology

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3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens

Article DOI: 10.3791/64651-v • 05:14 min • February 10th, 2023
February 10th, 2023 •
1, 1
1Department of Biology, University of Louisville

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This manuscript presents a detailed protocol to image the 3-D cell wall dynamics of living moss tissue, allowing the visualization of the detachment of cell walls in ggb mutants and thickening cell wall patterns in the wild type during development over a long period.

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3-D Time-lapse Imaging Cell Wall Dynamics Calcofluor Moss Physcomitrium Patens Fluorescence Microscopy Growth And Development Cellular Level Subcellular Level Protocol Calcofluor Dye Cell Wall Thickness Transformation Fluorescent Protein Calcofluor Signals Lasting Week Simple Structures Flowering Plants Staining Medium Moss Tissue Sterilized Water Wild Type Ggb Mutant AP-1000 Pipette BCDATG BCDAT Medium Glass Base Dish
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