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JoVE Journal Bioengineering
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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Article DOI: 10.3791/64674-v • 05:58 min • October 13th, 2023
October 13th, 2023 •
1, 1, 2, 1, 3, 4
1School of Microelectronics and Data Science, Anhui University of Technology, 2Shanghai Key Laboratory of Multidimensional Information Processing, East China Normal University, 3School of Chemistry and Chemical Engineering, Anhui University of Technology, 4Department of Medical Oncology, Changzheng Hospital

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The clinical microfluidic chip is an important biomedical analysis technique that simplifies clinical patient blood sample preprocessing and immunofluorescently stains circulating tumor cells (CTCs) in situ on the chip, allowing the rapid detection and identification of a single CTC.

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Clinical Microfluidic Chip Platform Versatile Circulating Tumor Cells Tumor Cell Enumeration Clinical CTC Isolation Culture Tumor Cells MCF-7 MDA-MB-231 HeLa Cell-culture Flask DMEM Fetal Bovine Serum Penicillin-streptomycin Trypsin Solution Calcein AM Incubator PBS Cell-counting Chamber Microfluidic Chip Syringe Pump Flow Rates Capture Efficiency
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