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Biochemistry

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JoVE Journal Biochemistry
Essai de sédimentation basé sur la concanavaline A pour mesurer la liaison au substrat des phosphatases glucanes
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Essai de sédimentation basé sur la concanavaline A pour mesurer la liaison au substrat des phosphatases glucanes

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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Cette méthode décrit un essai de sédimentation in vitro à base de lectine pour quantifier l’affinité de liaison de la phosphatase glucane et de l’amylopectine. Ce test de co-sédimentation est fiable pour mesurer la liaison du substrat de la phosphatase glucane et peut être appliqué à divers substrats de glucane solubilisés.

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rétractation numéro 190
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