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Cancer Research

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JoVE Journal Cancer Research
Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
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Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Article DOI: 10.3791/64713-v • 09:59 min • March 31st, 2023
March 31st, 2023 •
*1,3,4, *2,3,4, 1,3,4, 1,2,3,4
1Department of Chemistry, University of Illinois Urbana-Champaign, 2Department of Biochemistry, University of Illinois Urbana-Champaign, 3Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 4Cancer Center of Illinois, University of Illinois Urbana-Champaign
* These authors contributed equally

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Methods to measure ALDH1A1 activity in live cells are critical in cancer research due to its status as a biomarker of stemness. In this study, we employed an isoform-selective fluorogenic probe to determine the relative levels of ALDH1A1 activity in a panel of five ovarian cancer cell lines.

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AlDeSense Stratify Ovarian Cancer Cells Aldehyde Dehydrogenase 1A1 Activity Stem Cell Biomarker Flow Cytometry Live Cell Molecular Imaging Probe Activation High Signal To Background Ratio Selective ALDH1A1 Isoform Detection Michael Lee Sarah Gardner Rodrigo Tapia Hernandez Graduate Students Growth Media Eight Well Chamber Slide Serum Free Media Fluorogenic Probe Non-reactive Control Probe Confocal Microscope Imaging FITC Channel Transmitted Light Channels Z Plane Focus Laser Power FITC Gain
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