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Mouse In Vivo Placental Targeted CRISPR Manipulation
JoVE Journal
Genetica
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JoVE Journal Genetica
Mouse In Vivo Placental Targeted CRISPR Manipulation
DOI:

07:39 min

April 14, 2023

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Capitoli

  • 00:04Introduction
  • 00:48Surgery Preparation and Uterine Laparotomy
  • 02:34Placental Injection and Electroporation
  • 04:55Results: A Targeted In Vivo CRISPR Manipulation of the Mouse Placenta
  • 07:01Conclusion

Summary

Traduzione automatica

Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.

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