Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Voltage-Dependent Potassium Current Recording on…
JoVE Journal
Medicine

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Medicine
Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)
Journal (Medicine)
A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)
Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts
Journal (Medicine)
Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts
 

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Article DOI: 10.3791/64805-v • 08:11 min • November 11th, 2022
November 11th, 2022 •
*1, *1, 1, 2, 3,4, 5, 1,3,4, 4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

The present protocol describes an efficient method for the real-time and dynamic acquisition of voltage-gated potassium (Kv) channel currents in H9c2 cardiomyocytes using the whole-cell patch-clamp technique.

Tags

Voltage-dependent Potassium Current H9c2 Cardiomyocytes Whole-cell Patch-clamp Technique Aconitine Effects Ion Channels Cardiotoxicity Mechanism Pharmacological Mechanisms Toxic Mechanisms Specific Ion Channels Multisystem Diseases Cardiovascular System Nervous System Trypsin Digestion Cell Culture Glass Plates Micropipette Puller Borosilicate Glass Capillary Filament Ramp Program Electrode Fabrication
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter