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Journal / Biochemistry / Probing RNA Structure with Dimethyl Sulfate…
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Biochemistry

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.

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RNA Structure Dimethyl Sulfate Mutational Profiling Sequencing DMS-MaP Method Crystallography Electron Microscopy RNA Structure Ensembles Biological Research Refolding Buffer Nuclease-free Water PCR Tube Thermocycler Denature RNA Ice Block Refold RNA Dimethyl Sulfate (DMS) Concentration
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