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Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids
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JoVE Journal Ricerca sul cancro
Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

DOI:
10.3791/64878-v

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07:19 min

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January 06, 2023

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, , , , , , ,
1Washington University in St. Louis, 2University of California San Francisco

Capitoli

  • 00:05Introduction
  • 00:51Harvesting Organoids from Tissue
  • 03:58Passaging of Organoids
  • 05:05Freezing and Thawing of Organoids
  • 06:22Results: Generating Patient-Derived Ovarian Cancer Organoids from Patient Tumors, Ascites, and Pleural Fluid Samples
  • 06:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

Patient-derived organoids (PDO) are a three-dimensional (3D) culture that can mimic the tumor environment in vitro. In high-grade serous ovarian cancer, PDOs represent a model to study novel biomarkers and therapeutics.

Tags

Ovarian CancerPatient-derived OrganoidsTumor MicroenvironmentCell-cell InteractionsNovel TherapeuticsPredictive BiomarkersTumor HeterogeneityCollagenaseDNaseCell FiltrationCell PelletRBC Lysis

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