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Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
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JoVE Journal Ricerca sul cancro
Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

DOI:
10.3791/64881-v

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04:07 min

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February 24, 2023

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, , , , , , ,
1Washington University in St. Louis, 2University of California San Francisco

Capitoli

  • 00:04Introduction
  • 00:50Immunofluorescence Staining
  • 02:58Results: Evaluation of DNA Damage Repair Proteins in Organoids
  • 03:34Conclusion

Summary

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Traduzione automatica

The present protocol describes methods for evaluating DNA damage repair proteins in patient-derived ovarian cancer organoids. Included here are comprehensive plating and staining methods, as well as detailed, objective quantification procedures.

Tags

DNA Damage RepairOvarian CancerOrganoidsImmunofluorescence AssayBiomarkersPARP InhibitorsChemotherapeutic Resistance3D CulturesAnti-DNA Damage Repair Protein AntibodiesDAPIMicroscopySample Preparation

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