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Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Article DOI: 10.3791/65105-v • 07:40 min • May 26th, 2023
May 26th, 2023 •
1, 1, 1, 1, 1, 1, 1,2
1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Fangshan Chinese Medicine Hospital, Beijing University of Chinese Medicine

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Based on in vitro experiments, this study revealed the mechanism of crocetin in repairing oxidative stress damage of cardiomyocytes by influencing mitophagy, in which the PINK1/Parkin signaling pathway plays an important role.

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H9c2 Myocardial Cells Oxidative Stress Crocetin PINK1/Parkin Pathway Mitophagy Autophagy Autophagic Flux Experimental Procedures Infection Concentration Safety Measures Centrifugation DMEM Complete Medium Dimethyl Sulfoxide Control Group Crocetin Concentration 96-well Plate Incubation PBS Wash MTS Assay Cell Viability ROS Measurement DCFH-DA Dilution Serum-free Medium
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