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Journal / Medicine / Isolering av humana endotelceller i saphenös ven…
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JoVE Journal Medicine
Isolering av humana endotelceller i saphenös ven och exponering för kontrollerade nivåer av skjuvspänning och sträckning
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Isolering av humana endotelceller i saphenös ven och exponering för kontrollerade nivåer av skjuvspänning och sträckning

Article DOI: 10.3791/65122-v • 09:10 min • April 21st, 2023
April 21st, 2023 •
*1,2, *1, 1, 1, 1, 1, 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
* These authors contributed equally

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Vi beskriver ett protokoll för att isolera och odla humana saphenösa venendotelceller (hSVEC). Vi tillhandahåller också detaljerade metoder för att producera skjuvspänning och stretch för att studera mekanisk spänning i hSVEC.

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Denna månad i JoVE nummer 194
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