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Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
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Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Article DOI: 10.3791/65123-v • 10:23 min • May 19th, 2023
May 19th, 2023 •
1,2,3, 2,3,4, 1, 2, 2, 2,5, 2,3,5, 1,2,3,5
1Programa de Pós-Graduação em Biotecnologia da Universidade Federal do Amazonas - UFAM, 2Instituto Leônidas e Maria Deane (ILMD), Fundação Oswaldo Cruz (FIOCRUZ), 3Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro do Instituto Leônidas e Maria Deane (ILMD), Fundação Oswaldo Cruz (FIOCRUZ), 4Programa de Pós-graduação em Ciências Aplicadas à Hematologia – Universidade Estadual do Amazonas/Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas, 5Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas (UFAM)

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Summary
Automatic Translation

This protocol describes a methodology for the preparation of latex beads for assays using IgY antibodies for antigen detection.

Tags

Cytometric Bead Assay Egg-yolk Antibodies Immunoassay Techniques Flow Cytometry Latex Beads IgY Antibodies Detection Tool Sodium Hypochlorite Gallus Gallus Deckle White Lineage Yolk Separator Conical Tube Minus 20 Degrees Celsius One Millimolar PBS Hydrochloric Acid Centrifuge Supernatant Cellulose Filter
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