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Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

Article DOI: 10.3791/65156-v • 06:45 min • February 24th, 2023
February 24th, 2023 •
1, 1
1Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zurich

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Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.

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Live Imaging Arabidopsis Pollen Tube Reception Double Fertilization Semi-in Vitro Cum Septum Method Sexual Reproduction Gametophyte Interactions Ovules Sample Size Dissection Technique Steady Hand Pollen Germination Medium Agar Glass Bottom Dish Aluminum Block Humidity Chamber Arabidopsis Plants Septum Donors Stigma Donors
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