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Whole Neonatal Cochlear Explants as an In vitro Model

Article DOI: 10.3791/65160-v • 07:12 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1, 1,2
1Department of Biomedicine, University of Basel Hospital, 2Department of Otorhinolaryngology, Head and Neck Surgery, University of Basel Hospital

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The current protocol updates previous protocols and incorporates relatively straightforward approaches for culturing high-quality cochlear explants. This provides reliable data acquisition and high-resolution imaging in live and fixed cells. This protocol supports the ongoing trend of studying inner ear cells.

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Neonatal Cochlear Explants In Vitro Model Culture Steps High-quality Explants Molecular Mechanisms Cochlear Cells Organ Handling Dissection Steps Polymer Surface Coating Optimized Medium Volume Attachment Time Sensorineural Hearing Loss Ototoxic Models Structural Analysis Signaling Pathways Drug Screening Studies Inner Ear Research Embryonic Developmental Stages Decapitated Rat's Head Mandible Removal Skin Peeling Skull Cutting Cochlea Localization Superior Semicircular Canal
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