A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

Genri Numata; Eiki Takimoto

doi:10.3791/65384

Una procedura controllata dalla stimolazione per la valutazione delle funzioni diastoliche dipend...

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A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

Una procedura controllata dalla stimolazione per la valutazione delle funzioni diastoliche dipendenti dalla frequenza cardiaca in modelli murini di insufficienza cardiaca

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07:49 min

July 21, 2023

DOI: 10.3791/65384-v

Genri Numata^1,2, Eiki Takimoto^1,3

¹Department of Cardiovascular Medicine,The University of Tokyo Hospital, ²Department of Therapeutic Strategy for Heart Failure, Graduate School of Medicine,The University of Tokyo, ³Division of Cardiology, Department of Medicine,The Johns Hopkins Medical Institutions

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Overview

This study presents a protocol for assessing the pressure-volume relationship through transesophageal pacing in mouse models of heart failure, particularly focusing on the diastolic function. The protocol is designed to detect subtle changes in heart performance related to heart rate.

Key Study Components

Research Area

Cardiac function assessment
Heart failure models
Diastolic dysfunction evaluation

Background

Heart rate influence on cardiac characteristics
Limitations of treadmill exercise testing
Need for improved assessment methods for heart function

Methods Used

Atrial pacing protocol
Mouse models of heart failure
Transesophageal pacing technology

Main Results

Successfully identified cardiac dysfunction linked to heart rate changes
Demonstrated subtle performance changes in heart function
Validated the approach for studying diastolic dysfunction

Conclusions

The protocol effectively evaluates diastolic function in heart failure models
Offers a novel approach to studying heart rate-dependent cardiac functions

Frequently Asked Questions

What is the main objective of this study?

To develop a protocol that assesses the pressure-volume relationship in heart failure models.

How does this protocol improve upon traditional methods?

It addresses the limitations of treadmill testing by providing a more precise assessment of heart rate-dependent cardiac function.

What model system is used in this research?

Mouse models of heart failure are utilized for the experiments.

What does transesophageal pacing involve?

It is a technique used to assess heart function through pacing the heart via the esophagus.

Why is heart rate important in this study?

Heart rate changes are critical for understanding cardiac dysfunction, particularly in HFpEF.

What are the implications of this research?

This study offers new insights into the evaluation of diastolic dysfunction in heart failure, which can inform future studies.

How might this research impact future cardiac studies?

By providing a reliable method for assessing heart function, it may lead to better understanding and treatment of heart failure.

Il presente protocollo descrive l'ottenimento della relazione pressione-volume attraverso la stimolazione transesofagea, che funge da strumento prezioso nella valutazione della funzione diastolica in modelli murini di insufficienza cardiaca.

La relazione correlata alla frequenza cardiaca è importante per determinare le caratteristiche cardiache, in particolare nell'HFpEF. Questo protocollo ha lo scopo di valutare la funzione cardiaca dipendente dalla frequenza cardiaca. Eventuali modelli sperimentali, modalità disponibili per valutare la funzione cardiaca correlata alla frequenza cardiaca, sono limitati ai test da sforzo su tapis roulant.

Il test sul tapis roulant, tuttavia, è influenzato da variabili come il peso corporeo. Per superare queste limitazioni, abbiamo sviluppato un protocollo di stimolazione atriale per rilevare cambiamenti sottili ma significativi nelle prestazioni successive che sono collegati alla frequenza cardiaca. Il nostro protocollo offre uno strumento variabile per identificare le disfunzioni cardiache, che si manifestano con variazioni della frequenza cardiaca.

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