Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component

Francesca Truzzi; Silvia Dilloo; Xinyue Chang; Anne Whittaker; Eros D&#39;Amen; Giovanni Dinelli

doi:10.3791/65484

Sistema modello intestinale tridimensionale (3D) di base con una componente immunitaria

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component

Sistema modello intestinale tridimensionale (3D) di base con una componente immunitaria

Full Text

1,952 Views

07:39 min

September 1, 2023

DOI: 10.3791/65484-v

Francesca Truzzi¹, Silvia Dilloo¹, Xinyue Chang¹, Anne Whittaker¹, Eros D'Amen¹, Giovanni Dinelli¹

¹Department of Agricultural and Food Sciences,Alma Mater Studiorum-University of Bologna

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study presents a three-dimensional (3D) intestinal cell line model system to investigate the effects of various compounds on intestinal health. Using this model, researchers demonstrated that plant-derived compounds like spermidine and eugenol can enhance beneficial cellular processes, while gluten from specific wheat varieties may harm intestinal tissues.

Key Study Components

Research Area

Cell biology
Intestinal health and pathology
Drug screening

Background

Need for improved in vitro systems that reflect human physiology.
Challenges associated with traditional animal models.
Importance of standardized and reproducible models.

Methods Used

Three-dimensional cell line coculture of enterocytes, fibroblasts, and immune cells.
Assessment of cellular responses to treatments.
Paraffin embedding for histological analysis.

Main Results

Increased autophagy and inflammation with plant compounds.
Decreased epithelial layer thickness and disrupted cellular monolayer with high gluten content.
Activation and differentiation of monocytes when exposed to gluten.

Conclusions

The study provides a reliable in vitro model for investigating intestinal responses to dietary components.
Findings are relevant for understanding the impacts of diet on human intestinal health.

Frequently Asked Questions

What is the significance of using a 3D intestinal model?

It provides a more accurate representation of human intestinal physiology compared to traditional 2D systems.

How do spermidine and eugenol affect intestinal cells?

These compounds show beneficial effects by enhancing autophagy and modulating inflammation.

What impact does gluten have on intestinal tissues?

High gluten content can damage cell layers and activate immune responses in the intestinal environment.

Why is this model preferred over animal studies?

This human cell line model allows for standardized, reproducible results that are more applicable to human health.

What techniques were used for histological evaluation?

A paraffin embedding protocol was utilized to prepare samples for light microscopic analysis after cellular treatments.

What are the potential applications of this model?

It can be used for preclinical drug screening and investigating the effects of dietary components on intestinal health.

How do the findings contribute to understanding intestinal diseases?

The model allows researchers to explore the cellular mechanisms underlying inflammatory bowel diseases and food sensitivities.

Qui descriviamo la costruzione di un sistema modello di linea cellulare intestinale tridimensionale (3D) di base e un protocollo di inclusione della paraffina per la valutazione microscopica ottica di equivalenti intestinali fissi. La colorazione di proteine selezionate consente l'analisi di più parametri visivi da un singolo esperimento per un potenziale utilizzo in studi preclinici di screening farmacologico.

Lo scopo del lavoro è quello di utilizzare questi modelli tridimensionali in vitro di linee cellulari umane per studiare il fatto di composti benefici e utili sul tessuto intestinale. I modelli possono essere utilizzati per approfondire la comprensione della ricerca e anche come modello farmacologico di base. Utilizzando i composti di origine vegetale, spermidina ed eugenolo, sia separatamente che insieme sotto forma di integratore, misuriamo gli aumenti benefici dell'autofagia e la diminuzione dell'infiammazione utilizzando questi modelli.

Successivamente saremo anche in grado di dimostrare l'effetto potenzialmente dannoso del glutine estratto da alcune varietà di grano sui tessuti intestinali. Attualmente stiamo utilizzando sistemi in vitro a prezzi economici, standardizzati e facilmente ripetibili che riflettono meglio la fisiologia dell'intestino umano come alternativa ai modelli animali. Poiché utilizziamo anche linee cellulari umane commerciali piuttosto che cellule di origine vegetale, le risposte dello studio sono più applicabili alla popolazione generale.

View the full transcript and gain access to thousands of scientific videos