Bulk Electroacupuncture Operation for Mice or Young Rats

Siqi Yao; Yun Wei; Yuping Zhang; Xiaolin Yang; Zhifeng Wu

doi:10.3791/65648

Operazione di elettroagopuntura di massa per topi o ratti giovani

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Bulk Electroacupuncture Operation for Mice or Young Rats

Operazione di elettroagopuntura di massa per topi o ratti giovani

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04:06 min

September 1, 2023

DOI: 10.3791/65648-v

Siqi Yao*¹, Yun Wei*¹, Yuping Zhang¹, Xiaolin Yang², Zhifeng Wu¹

¹Department of Pediatrics, Second Affiliated Hospital,Army Medical University, ²Department of Neurosurgery, Second Affiliated Hospital,Army Medical University

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study presents an efficient protocol for performing electroacupuncture (EA) on multiple awake mice or young rats using a custom-designed three-dimensional (3D) printed holder. This innovative approach enhances operational efficiency, saves time, and improves experimental success rates during electroacupuncture procedures.

Key Study Components

Research Area

Electroacupuncture techniques
Animal welfare in research
Experimental efficiency

Background

Traditional acupuncture techniques face challenges with uncooperative animals.
Anesthesia poses clinical applicability issues for acupuncture.
Efficient methods are needed for performing procedures on multiple subjects.

Methods Used

Electroacupuncture
Mice and young rats
3D printed holder for simultaneous treatment

Main Results

The use of a 3D printed holder allows for simultaneous treatment of multiple animals.
Increased efficiency and safety compared to traditional methods that require immobilization.
Demonstrates improved experimental success rates.

Conclusions

The study showcases a novel method for electroacupuncture that is more efficient and user-friendly.
This approach significantly contributes to the advancement of biological research techniques involving animals.

Frequently Asked Questions

What are the advantages of using a 3D printed holder in electroacupuncture?

The holder allows simultaneous treatment of multiple animals, enhancing operational efficiency and improving success rates.

Can this method be applied to other species?

While this study focuses on mice and young rats, the principles may be adapted for use in other species with appropriate modifications.

Is anesthesia required for this electroacupuncture method?

No, this method is designed to be used on awake animals without the need for anesthesia.

How does this method impact animal welfare?

It minimizes the need for immobilization, which can reduce stress and discomfort in the animals.

Who can operate the 3D printed device?

The device is designed to be user-friendly and can be operated by a single person.

What types of experiments benefit from this method?

Experiments requiring accurate and efficient application of electroacupuncture techniques will greatly benefit from this method.

Are there any limitations to this protocol?

Further research may be required to optimize the design for other types of animals or different electroacupuncture techniques.

Qui, presentiamo un protocollo per l'elettroagopuntura (EA) efficiente e rapida nei topi o nei ratti giovani utilizzando un supporto tridimensionale (3D) stampato. Questa tecnica consente di operare contemporaneamente su più animali, risparmiando tempo e aumentando l'efficienza sperimentale.

In questo video, presentiamo un modo efficiente di utilizzare l'elettroagopuntura su più topi o ratti svegli. Nella ricerca vescicale, gli animali urinano, ma non sono collaborativi, e l'agopuntura consuma solo tempo. Un altro problema riguarda l'anestesia, che non è applicabile clinicamente all'agopuntura.

Per affrontare questi problemi, dimostriamo un metodo efficiente per eseguire l'elettroagopuntura su più topi o ratti svegli utilizzando un supporto stampato in 3D. Il nostro protocollo dimostra un modo più efficiente e sicuro per tenere più animali contemporaneamente, che a differenza di altri metodi che implicano l'immobilizzazione, questo metodo non solo consente di risparmiare tempo, ma migliora anche le percentuali di successo sperimentale. Inoltre, il dispositivo è progettato per essere facile da maneggiare, che potrebbe essere azionato da una sola persona.

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