Name: Uno strumento per creare automaticamente gap stabili e riproducibili senza cellule per migliorare laffidabilità del saggio di guarigione delle ferite cellulari (Video) | JoVE Journal
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Description: Scopri un raschietto cellulare innovativo per migliorare i saggi di guarigione delle ferite con maggiore riproducibilità e affidabilità. Questo metodo preserva l'integrità cellulare e ottimizza l'esperienza utente nella ricerca biologica.

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<meta charset="utf8"></head><body>Innovazione di un raschietto cellulare migliorato per l'avvolgimento delle cellule mediante l'analisi dei requisiti dell'utente

<ol><li>Trepat, X., Chen, Z., Jacobson, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cell+migration%5BTitle%5D)+AND+%22Compr+Physiol%22%5BJournal%5D)">Cell migration.</a> Compr Physiol. 2 (4), 2369-2392 (2012).</li>
<li>Moncharmont, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Radiation-enhanced+cell+migration%2Finvasion+process%3A+a+review%5BTitle%5D)+AND+%22Crit+Rev+Oncol+Hematol%22%5BJournal%5D)">Radiation-enhanced cell migration/invasion process: a review.</a> Crit Rev Oncol Hematol. 92 (2), 133-142 (2014).</li>
<li>Verbeek, B. S., Adriaansen-Slot, S. S., Vroom, T. M., Beckers, T., Rijksen, G. J. F. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Overexpression+of+EGFR+and+c-erbB2+causes+enhanced+cell+migration+in+human+breast+cancer+cells+and+NIH3T3+fibroblasts%5BTitle%5D)+AND+%22FEBS+Lett%22%5BJournal%5D)">Overexpression of EGFR and c-erbB2 causes enhanced cell migration in human breast cancer cells and NIH3T3 fibroblasts.</a> FEBS Lett. 425 (1), 145-150 (1998).</li>
<li>Vaideeswar, P., Aswani, Y., Damani, S., Singaravel, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pulmonary+microvascular+metastases+in+cervical+carcinoma%3A+A+case+series%5BTitle%5D)+AND+%22J+Postgrad+Med%22%5BJournal%5D)">Pulmonary microvascular metastases in cervical carcinoma: A case series.</a> J Postgrad Med. 66 (3), 155-158 (2020).</li>
<li>Liang, Y., Zhang, H., Song, X., Yang, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metastatic+heterogeneity+of+breast+cancer%3A+Molecular+mechanism+and+potential+therapeutic+targets%5BTitle%5D)+AND+%22Semin+Cancer+Biol%22%5BJournal%5D)">Metastatic heterogeneity of breast cancer: Molecular mechanism and potential therapeutic targets.</a> Semin Cancer Biol. 60, 14-27 (2020).</li>
<li>Sasaki, R., Osaki, M., Okada, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((MicroRNA-based+diagnosis+and+treatment+of+metastatic+human+osteosarcoma%5BTitle%5D)+AND+%22Cancers+%28Basel%29%22%5BJournal%5D)">MicroRNA-based diagnosis and treatment of metastatic human osteosarcoma.</a> Cancers (Basel). 11 (4), 553(2019).</li>
<li>Spaeth, E., Klopp, A., Dembinski, J., Andreeff, M., Marini, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inflammation+and+tumor+microenvironments%3A+defining+the+migratory+itinerary+of+mesenchymal+stem+cells%5BTitle%5D)+AND+%22Gene+Ther%22%5BJournal%5D)">Inflammation and tumor microenvironments: defining the migratory itinerary of mesenchymal stem cells.</a> Gene Ther. 15 (10), 730-738 (2008).</li>
<li>Zhang, M., Li, H., Ma, H., Qin, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simple+microfluidic+strategy+for+cell+migration+assay+in+an+in+vitro+wound-healing+model%5BTitle%5D)+AND+%22Wound+Repair+Regen%22%5BJournal%5D)">A simple microfluidic strategy for cell migration assay in an in vitro wound-healing model.</a> Wound Repair Regen. 21 (6), 897-903 (2013).</li>
<li>Yue, P. Y., Leung, E. P., Mak, N., Wong, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simplified+method+for+quantifying+cell+migration%2Fwound+healing+in+96-well+plates%5BTitle%5D)+AND+%22J+Biomol+Screen%22%5BJournal%5D)">A simplified method for quantifying cell migration/wound healing in 96-well plates.</a> J Biomol Screen. 15 (4), 427-433 (2010).</li>
<li>Roch, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunological+evaluation+of+polystyrene+and+poly+%28ether+imide%29+cell+culture+inserts+with+different+roughness%5BTitle%5D)+AND+%22Clin+Hemorheol+Microcirc%22%5BJournal%5D)">Immunological evaluation of polystyrene and poly (ether imide) cell culture inserts with different roughness.</a> Clin Hemorheol Microcirc. 52 (2-4), 375-389 (2012).</li>
<li>Jonkman, J. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+introduction+to+the+wound+healing+assay+using+live-cell+microscopy%5BTitle%5D)+AND+%22Cell+Adh+Migr%22%5BJournal%5D)">An introduction to the wound healing assay using live-cell microscopy.</a> Cell Adh Migr. 8 (5), 440-451 (2014).</li>
<li>Freshney, R. I. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aFreshney%2C+RI+%22Culture+of+animal+cells%3A+a+manual+of+basic+technique+and+specialized+applications%22">Culture of animal cells: a manual of basic technique and specialized applications</a>. , John Wiley & Sons, Inc. (2015).</li>
<li>Kato, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reuse+of+cell+culture+inserts+for+in+vitro+human+primary+airway+epithelial+cell+studies%5BTitle%5D)+AND+%22Am+J+Respir+Cell%22%5BJournal%5D)">Reuse of cell culture inserts for in vitro human primary airway epithelial cell studies.</a> Am J Respir Cell. 64 (6), 760-764 (2021).</li>
<li>Veludo-de-Oliveira, T. M., Ikeda, A. A., Campomar, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Discussing+laddering+application+by+the+means-end+chain+theory%5BTitle%5D)+AND+%22Qualit+Rep%22%5BJournal%5D)">Discussing laddering application by the means-end chain theory.</a> Qualit Rep. 11 (4), 626-642 (2006).</li>
<li>Yang, T., Yang, F., Men, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recommendation+content+matters%21+Exploring+the+impact+of+the+recommendation+content+on+consumer+decisions+from+the+means-end+chain+perspective%5BTitle%5D)+AND+%22Int+J+Info+Manage%22%5BJournal%5D)">Recommendation content matters! Exploring the impact of the recommendation content on consumer decisions from the means-end chain perspective.</a> Int J Info Manage. 68, 102589(2023).</li>
<li>Sadeghlou, S., Emami, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Residential+preferences+and+satisfaction%3A+a+qualitative+study+using+means-end+chain+theory%5BTitle%5D)+AND+%22J+Housing+Built+Env%22%5BJournal%5D)">Residential preferences and satisfaction: a qualitative study using means-end chain theory.</a> J Housing Built Env. 38, 1711-1734 (2023).</li>
<li>Pieters, R., Baumgartner, H., Allen, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+means-end+chain+approach+to+consumer+goal+structures%5BTitle%5D)+AND+%22Int+J+Res+Market%22%5BJournal%5D)">A means-end chain approach to consumer goal structures.</a> Int J Res Market. 12 (3), 227-244 (1995).</li>
<li>Valette-Florence, P., Rapacchi, B. J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improvements+in+means-end+chain+analysis%3A+using+graph+theory+and+correspondence+analysis%5BTitle%5D)+AND+%22J+Advert+Res%22%5BJournal%5D)">Improvements in means-end chain analysis: using graph theory and correspondence analysis.</a> J Advert Res. 31, 30-45 (1991).</li>
<li>Nunkoo, R., Ramkissoon, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applying+the+means-end+chain+theory+and+the+laddering+technique+to+the+study+of+host+attitudes+to+tourism%5BTitle%5D)+AND+%22J+Sustain+Tourism%22%5BJournal%5D)">Applying the means-end chain theory and the laddering technique to the study of host attitudes to tourism.</a> J Sustain Tourism. 17 (3), 337-355 (2009).</li>
<li>Development of an automatic mold polishing system. Tsai, M. J., Chang, J. L., Haung, J. F. IEEE Int Conf Robot Auto, 3, 3517-3522 (2005).</li>
<li>Altan, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advanced+techniques+for+die+and+mold+manufacturing%5BTitle%5D)+AND+%22CIRP+Annals%22%5BJournal%5D)">Advanced techniques for die and mold manufacturing.</a> CIRP Annals. 42 (2), 707-716 (1993).</li>
<li>Kalt, E., Monfared, R., Jackson, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Towards+an+automated+polishing+system%3A+Capturing+manual+polishing+operations%5BTitle%5D)+AND+%22Int+J+Res+Eng+Tech%22%5BJournal%5D)">Towards an automated polishing system: Capturing manual polishing operations.</a> Int J Res Eng Tech. 5 (7), 182-192 (2016).</li>
<li>Becerra, L. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aBecerra%2C+L+%22CMF+design%3A+the+fundamental+principles+of+colour%2C+material+and+finish+design%22">CMF design: the fundamental principles of colour, material and finish design</a>. , Frame Publishers, UK. (2016).</li>
<li>Pan, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Next-generation+immuno-oncology+agents%3A+current+momentum+shifts+in+cancer+immunotherapy%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Next-generation immuno-oncology agents: current momentum shifts in cancer immunotherapy.</a> J Hematol Oncol. 13 (1), 29(2020).</li>
<li>Kim, S., Nah, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+development+direction+of+emotional+materials+by+increasing+sensorial+experiences-Focusing+on+the+case+study+of+CMF+design%5BTitle%5D)+AND+%22Arch+Des+Res%22%5BJournal%5D)">The development direction of emotional materials by increasing sensorial experiences-Focusing on the case study of CMF design.</a> Arch Des Res. 27 (2), 121-135 (2014).</li>
<li>Eiseman, L. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aEiseman%2C+L+%22The+complete+color+harmony%2C+pantone+edition%3A+expert+color+information+for+professional+results%22">The complete color harmony, pantone edition: expert color information for professional results</a>. , Rockport Publishers. (2017).</li>
<li>Eiseman, L., Recker, K. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aEiseman%2C+L+%22Pantone%3A+The+twentieth+century+in+color%3A%28coffee+table+books%2C+design+books%2C+best+books+about+color%29%22">Pantone: The twentieth century in color:(coffee table books, design books, best books about color)</a>. , Chronicle Books. (2011).</li>
<li>Deng, P., Jin, W., Liu, Z., Gao, M., Zhou, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Novel+multifunctional+adenine-modified+chitosan+dressings+for+promoting+wound+healing%5BTitle%5D)+AND+%22Carbohydr+Polym%22%5BJournal%5D)">Novel multifunctional adenine-modified chitosan dressings for promoting wound healing.</a> Carbohydr Polym. 260, 117767(2021).</li>
<li>Ares, G., Giménez, A., Gámbaro, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Understanding+consumers%27+perception+of+conventional+and+functional+yogurts+using+word+association+and+hard+laddering%5BTitle%5D)+AND+%22Food+Quality+Prefer%22%5BJournal%5D)">Understanding consumers' perception of conventional and functional yogurts using word association and hard laddering.</a> Food Quality Prefer. 19 (7), 636-643 (2008).</li>
<li>Lee, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+study+on+word+cloud+techniques+for+analysis+of+unstructured+text+data%5BTitle%5D)+AND+%22J+Converg+Culture+Tech%22%5BJournal%5D)">A study on word cloud techniques for analysis of unstructured text data.</a> J Converg Culture Tech. 6 (4), 715-720 (2020).</li>
<li>Word Cloud Explorer: Text Analytics Based on Word Clouds. Heimerl, F., Lohmann, S., Lange, S., Ertl, T. 47th Hawaii international conference on system sciences, , IEEE. (2014).</li>
<li>Kulevicz, R. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+sustainability+reports+on+social+and+environmental+issues%3A+bibliometric+analysis+and+the+word+cloud+approach%5BTitle%5D)+AND+%22Env+Rev%22%5BJournal%5D)">Influence of sustainability reports on social and environmental issues: bibliometric analysis and the word cloud approach.</a> Env Rev. 28 (4), 380-386 (2020).</li>
<li>Rubbo, S. D., Gardner, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+review+of+sterilization+and+disinfection%5BTitle%5D)+AND+%22Lloyd-Luke%22%5BJournal%5D)">A review of sterilization and disinfection.</a> Lloyd-Luke. , (1965).</li>
<li>Kelsey, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sterilization+by+ethylene+oxide%5BTitle%5D)+AND+%22J+Clin+Pathol%22%5BJournal%5D)">Sterilization by ethylene oxide.</a> J Clin Pathol. 14 (1), 59-61 (1961).</li>
<li>Shintani, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ethylene+oxide+gas+sterilization+of+medical+devices%5BTitle%5D)+AND+%22Biocontrol+Sci%22%5BJournal%5D)">Ethylene oxide gas sterilization of medical devices.</a> Biocontrol Sci. 22 (1), 1-16 (2017).</li>
<li>Rutala, W. A., Gergen, M. F., Weber, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparative+evaluation+of+the+sporicidal+activity+of+new+low-temperature+sterilization+technologies%3A+ethylene+oxide%2C+2+plasma+sterilization+systems%2C+and+liquid+peracetic+acid%5BTitle%5D)+AND+%22Am+J+Infect+Control%22%5BJournal%5D)">Comparative evaluation of the sporicidal activity of new low-temperature sterilization technologies: ethylene oxide, 2 plasma sterilization systems, and liquid peracetic acid.</a> Am J Infect Control. 26 (4), 393-398 (1998).</li>
<li>Dion, M., Parker, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Steam+sterilization+principles%5BTitle%5D)+AND+%22Pharm+Eng%22%5BJournal%5D)">Steam sterilization principles.</a> Pharm Eng. 33 (6), 1-8 (2013).</li>
<li>Környei, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cell+sorting+in+a+Petri+dish+controlled+by+computer+vision%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Cell sorting in a Petri dish controlled by computer vision.</a> Sci Rep. 3, 1088(2013).</li>
<li>Hsu, J. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+wound+assessment+and+infection+detection+method%5BTitle%5D)+AND+%22BMC+Med+Inform+Decis+Mak%22%5BJournal%5D)">Chronic wound assessment and infection detection method.</a> BMC Med Inform Decis Mak. 19 (1), 99(2019).</li>
<li>Katoh, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Test-retest+reliability+of+isometric+ankle+plantar+flexion+strength+measurement+performed+by+a+hand-held+dynamometer+considering+fixation%3A+examination+of+healthy+young+participants%5BTitle%5D)+AND+%22J+Phys+Ther+Sci%22%5BJournal%5D)">Test-retest reliability of isometric ankle plantar flexion strength measurement performed by a hand-held dynamometer considering fixation: examination of healthy young participants.</a> J Phys Ther Sci. 34 (6), 463-466 (2022).</li>
<li>Li, X., Zhao, J., Ma, G., Huang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Experimental+study+on+the+traditional+timber+mortise-tenon+joints%5BTitle%5D)+AND+%22Adv+Str+Eng%22%5BJournal%5D)">Experimental study on the traditional timber mortise-tenon joints.</a> Adv Str Eng. 18 (12), 2089-2102 (2016).</li>
<li>Cottle, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+principal+pivoting+method+revisited%5BTitle%5D)+AND+%22Mathematical+Prog%22%5BJournal%5D)">The principal pivoting method revisited.</a> Mathematical Prog. 48, 369-385 (1990).</li>
<li>Cross, N. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aCross%2C+N+%22Engineering+design+methods%3A+strategies+for+product+design%22">Engineering design methods: strategies for product design</a>. , John Wiley & Sons. (2021).</li>
<li>Otto, K., Wood, K. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aOtto%2C+K+%22Product+design%3A+techniques+in+reverse+engineering+and+new+product+development%22">Product design: techniques in reverse engineering and new product development</a>. , Pearson. (2001).</li>
<li>Tariq, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gefitinib+inhibits+M2-like+polarization+of+tumor-associated+macrophages+in+Lewis+lung+cancer+by+targeting+the+STAT6+signaling+pathway%5BTitle%5D)+AND+%22Acta+Pharmacol+Sin%22%5BJournal%5D)">Gefitinib inhibits M2-like polarization of tumor-associated macrophages in Lewis lung cancer by targeting the STAT6 signaling pathway.</a> Acta Pharmacol Sin. 38 (11), 1501-1511 (2017).</li>
<li>Rahimi, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRISPR%2FCas9-mediated+knockout+of+Lcn2+effectively+enhanced+CDDP-induced+apoptosis+and+reduced+cell+migration+capacity+of+PC3+cells%5BTitle%5D)+AND+%22Life+Sci%22%5BJournal%5D)">CRISPR/Cas9-mediated knockout of Lcn2 effectively enhanced CDDP-induced apoptosis and reduced cell migration capacity of PC3 cells.</a> Life Sci. 231, 116586(2019).</li>
<li>Cormier, N., Yeo, A., Fiorentino, E., Paxson, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+the+wound+scratch+assay+to+detect+changes+in+murine+mesenchymal+stromal+cell+migration+after+damage+by+soluble+cigarette+smoke+extract%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Optimization of the wound scratch assay to detect changes in murine mesenchymal stromal cell migration after damage by soluble cigarette smoke extract.</a> J Vis Exp. (106), e53414(2015).</li>
<li>Pinto, B. I., Cruz, N. D., Lujan, O. R., Propper, C. R., Kellar, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+vitro+scratch+assay+to+demonstrate+effects+of+arsenic+on+skin+cell+migration%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">In vitro scratch assay to demonstrate effects of arsenic on skin cell migration.</a> J Vis Exp. (144), e58838(2019).</li>
<li>Rodriguez, L. G., Wu, X., Guan, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Wound+healing+assay%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Wound healing assay.</a> Methods Mol Biol. 294, 23-29 (2005).</li>
</ol>

<table><tbody><tr><td>Kit CCK-8</td><td>Beyotime Company, Cina</td> Sistema di microscopio digitale <td>C0037</td><td></td></tr> Siero <tr><td>fetale bovino</td><td></td><td>Olympus</td> IX81 Gibco, USA 16000044 <td></td></tr><tr><td></td><td></td><td></td> HOS&nbsp;<td></td></tr><tr><td></td><td>Procell Scienze della Vita & Technology Co., Ltd</td><td>CL-0360</td><td></td></tr><tr><td>Image-Pro Plus</td><td>Media Cybernetics</td><td>versione 6.0</td><td></td></tr><tr><td>KeyShot</td><td>Luxion</td><td>versione 11.0</td><td>Software di rendering 3D</td></tr><tr><td>lettore di micropiastre</td><td>BioTek, tedesco</td><td>ELX808</td><td></td></tr><tr><td>Minimum Essential Medium</td><td>Gibco, USA</td><td>11095080</td><td></td></tr><tr><td>Sistema di corrispondenza Pantone</td><td></td><td></td><td>corrispondenza dei colori commerciale</td></tr><tr><td>penicillina-streptomicina</td><td>Beyotime Company, Cina</td><td>ST488</td><td></td></tr><tr><td>Photoshop</td><td>Adobe</td><td></td><td>foto e software di progettazione</td></tr><tr><td>Rhinoceros 3D</td><td>Robert McNeel & Associates</td><td>versione 7.0</td><td>Software di progettazione 3D</td></tr><tr><td>TC20 Contatore di cellule automatizzato</td><td>Bio-Rad</td><td>TC20</td><td></td></tr><tr><td>Tripsina</td><td>Cytiva&nbsp; HyClone, Stati Uniti</td><td>SH30042.01</td><td></td></tr></tbody></table>

uno strumento per creare automaticamente gap stabili e riproducibili senza cellule per migliorare l'affidabilità del saggio di guarigione delle ferite cellulari

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

Uno strumento per creare automaticamente gap stabili e riproducibili senza cellule per migliorare l'affidabilità del saggio di guarigione delle ferite cellulari

The reliability of cellular migration measurement in wound healing assays is frequently undermined by the prevalent methodological instability, i.e., ...

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JoVE Journal

Biology

Biologia

Questo video descrive un test di guarigione delle ferite in co-coltura senza contatto per valutare la capacità migratoria dei mioblasti murini. Questo aiuta a comprendere i componenti di invio e ricezione del segnale delle interazioni di segnalazione Wnt non canoniche paracrine in vitro.

Saggio della ferita per valutare la capacità migratoria dei mioblasti murini in co-coltura

Comunemente utilizzati, sono descritti metodi altamente accessibili per l'esame migrazione cellulare e l'invasione in vitro. Il primo metodo è il saggio chiusura della ferita cella che misura la motilità cellulare. Il secondo metodo è il transwell migrazione e invasione test che valuta la chemiotassi e la capacità invasiva delle cellule.

Nella migrazione delle cellule in vitro e saggi Invasion

Questa presentazione dimostra un metodo in cui l'elettroporazione di cellule aderenti e coltivate viene utilizzata per lo studio della comunicazione intercellulare e giunzionale, mentre le cellule crescono su un vetrino rivestito con ossido di indio-stagno conduttivo e trasparente.

Un test funzionale per Gap giunzionale esame; Elettroporazione di cellule aderenti su indio-Tin Oxide

Qui, presentiamo un protocollo per eseguire un test di migrazione cellulare semi-high throughput su una piastra di coltura cellulare a 96 pozzetti. Questo protocollo è un metodo rapido, semplice ed economico per creare ferite scratch coerenti su un monostrato di cellule.

Un dispositivo per realizzare la migrazione delle cellule / Wound Healing in una piastra a 96 pozzetti

Questo manoscritto descrive come incisione-come le lesioni fatta su monostrati di cellule epiteliali in coltura convenientemente modello ferita curativa in vitro, che consente per l'imaging di confocal o microscopia di esame del laser, e che in grado di fornire alta qualità dati quantitativi e qualitativi per studiare il comportamento delle cellule sia i meccanismi coinvolti nella migrazione.

La microscopia basato su metodi per la valutazione della migrazione delle cellule epiteliali durante In Vitro la guarigione arrotolata

Qui, presentiamo un protocollo per valutare l'effetto dei peptidi sulla migrazione di cellule epiteliali bronchiali. Questo metodo consente l'ottenimento rapido e altamente riproducibile di dati quantitativi sulla velocità di chiusura della ferita e migrazione cellulare.

Un romanzo In Vitro ferita guarigione test per valutare la migrazione cellulare

Qui descriviamo una procedura per verificare la migrazione cellule in vitro con un microscopio ottico automatizzato fotocamera. Il dosaggio di gratta e Vinci è stato ampiamente usato dove la chiusura del graffio è seguita per un periodo determinato. Il microscopio ottico consente un rilevamento affidabile e a buon mercato della migrazione cellulare.

Ottimizzato Scratch test per test In Vitro della migrazione cellulare con una fotocamera ottica automatizzata

L'attuale protocollo descrive un metodo integrato, indagando la migrazione delle cellule tumorali e l'invasione su un'unica piattaforma in tempo reale, fornendo un'opzione facilmente riproducibile e tempo efficiente per studiare la morfologia e la mobilità delle cellule.

Un flusso di lavoro semplice migrazione/invasione utilizzando un Imager di vivere-cella automatizzata

Qui, presentiamo un protocollo per un saggio in vitro graffio utilizzando fibroblasti primari e per un saggio di guarigione della ferita della pelle in vivo nei topi. Entrambi i test sono metodi semplici per valutare la guarigione delle ferite in vitro e in vivo.

Scratch Migration Ssay and Dorsal Skinfold Chamber for In Vitro e In Vivo Analisi della Guarigione delle Ferite

Questo studio presenta due tecniche sperimentali cruciali: la costruzione di un modello di ferita murina per valutare la chiusura della ferita e la preparazione di sospensioni unicellulari dalla pelle murina per preservare la vitalità cellulare.

Modelli di ferita nel topo e preparazione di sospensioni a cellula singola

Scopri un raschietto cellulare innovativo per migliorare i saggi di guarigione delle ferite con maggiore riproducibilità e affidabilità. Questo metodo preserva l'integrità cellulare e ottimizza l'esperienza utente nella ricerca biologica.

Uno strumento per creare automaticamente gap stabili e riproducibili senza cellule per migliorare laffidabilità del saggio di guarigione delle ferite cellulari (Video) | JoVE Journal

Uno strumento per creare automaticamente gap stabili e riproducibili senza cellule per migliorare l'affidabilità del saggio di guarigione delle ferite cellulari

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