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A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development (Video) | JoVE

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

Article DOI: 10.3791/65828-v • 01:55 min • August 25th, 2023
August 25th, 2023 •
1, 1, 1,2, 1
1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 2Department of Biochemistry, Faculty of Medicine and Dentistry, University of Alberta

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ABCG5/G8 Lipide Bicelle Röntgenkristallografie Membraaneiwitten Lipide dubbellaag Detergenten Stabiliteit Uniformiteit Structurele karakterisering Bicellen Fosfolipiden Hoogwaardige diffractiekristallen Structuur met hoge resolutie Oorspronkelijke conformatie Functionaliteit Driedimensionale kristallen In detergent opgeloste heterodimeer ABCG5/G8 DMPC/CHAPSO-bicellen Cholesterol Dampdiffusie Eiwitkristallisatie
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