Name: Studio multimodale del rimodellamento cardiovascolare murino: ecografia quadridimensionale e spettrometria di massa (Video) | JoVE Journal
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Description: Scopri l'imaging ecografico 4D in vivo e la spettrometria di massa per studiare il rimodellamento cardiovascolare murino. Questo approccio multimodale consente di analizzare le alterazioni biomeccaniche e biomolecolari nel sistema cardiovascolare.

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<meta charset="utf8"></head><body>Ecografia 4D e imaging MALDI per analisi cardiache e vascolari nei topi

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<li>Hattori, Y., Konno, H., Abe, M., Miyoshi, H., Goto, T., Makabe, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Synthesis%2C+determination+of+the+absolute+configuration+of+tonkinelin%2C+and+inhibitory+action+with+bovine+heart+mitochondrial+complex+I%5BTitle%5D)+AND+%22Bioorg+Med+Chem%22%5BJournal%5D)">Synthesis, determination of the absolute configuration of tonkinelin, and inhibitory action with bovine heart mitochondrial complex I.</a> Bioorg Med Chem. 15 (8), 3026-3031 (2007).</li>
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<li>Crouch, A. C., Castle, P. E., Fitzgerald, L., Scheven, U. M., Greve, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+structural+and+functional+response+of+murine+vasculature+to+acute+%CE%B2-adrenergic+stimulation+in+vivo+during+hypothermic+and+hyperthermic+conditions%5BTitle%5D)+AND+%22Int+J+Hyperthermia%22%5BJournal%5D)">Assessing structural and functional response of murine vasculature to acute β-adrenergic stimulation in vivo during hypothermic and hyperthermic conditions.</a> Int J Hyperthermia. 36 (1), 1136-1145 (2019).</li>
<li>Crouch, A. C., Manders, A. B., Cao, A. A., Scheven, U. M., Greve, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cross-sectional+area+of+the+murine+aorta+linearly+increases+with+increasing+core+body+temperature%5BTitle%5D)+AND+%22Int+J+Hyperthermia%22%5BJournal%5D)">Cross-sectional area of the murine aorta linearly increases with increasing core body temperature.</a> Int J Hyperthermia. 34 (7), 1121-1133 (2018).</li>
<li>Clift, C. L., Mehta, A., Drake, R. R., Angel, P. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multiplexed+imaging+mass+spectrometry+of+histological+staining%2C+N-glycan+and+extracellular+matrix+from+one+tissue+section%3A+A+tool+for+fibrosis+research%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Multiplexed imaging mass spectrometry of histological staining, N-glycan and extracellular matrix from one tissue section: A tool for fibrosis research.</a> Methods Mol Biol. 2350, 313-329 (2021).</li>
<li>Salehi, H. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coregistered+photoacoustic+and+ultrasound+imaging+and+classification+of+ovarian+cancer%3A+Ex+vivo+and+in+vivo+studies%5BTitle%5D)+AND+%22J+Biomed+Opt%22%5BJournal%5D)">Coregistered photoacoustic and ultrasound imaging and classification of ovarian cancer: Ex vivo and in vivo studies.</a> J Biomed Opt. 21 (4), 046006(2016).</li>
<li>Verbeeck, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Connecting+imaging+mass+spectrometry+and+magnetic+resonance+imaging-based+anatomical+atlases+for+automated+anatomical+interpretation+and+differential+analysis%5BTitle%5D)+AND+%22Biochim+Biophys+Acta%22%5BJournal%5D)">Connecting imaging mass spectrometry and magnetic resonance imaging-based anatomical atlases for automated anatomical interpretation and differential analysis.</a> Biochim Biophys Acta. 1865 (7), 967-977 (2017).</li>
<li>Yang, E. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Real-time+co-registration+using+novel+ultrasound+technology%3A+Ex+vivo+validation+and+in+vivo+applications%5BTitle%5D)+AND+%22J+Am+Soc+Echocardiogr%22%5BJournal%5D)">Real-time co-registration using novel ultrasound technology: Ex vivo validation and in vivo applications.</a> J Am Soc Echocardiogr. 24 (7), 720(2011).</li>
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<li>Lin, B. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((MSIr%3A+Automatic+registration+service+for+mass+spectrometry+imaging+and+histology%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">MSIr: Automatic registration service for mass spectrometry imaging and histology.</a> Anal Chem. 95 (6), 3317-3324 (2023).</li>
</ol>

<table><tbody><tr><td>Acido 2,5-diidrossibenzoico (DHB)</td><td>Supelco,&nbsp; >99,0% (HPLC)</td><td>85707-10MG-F</td><td>DHB sostanza della matrice per MALDI-MS; https://www.sigmaaldrich.com/US/en/product/sigma/85707?cm_sp=Insite-_-wimsShippingEmailRecs_wims EmailAPI_wimsGruCrossEntropy-_-wimsEmailAPI10-3</td></tr><tr><td>9-aminoacridina (9AA)</td><td>Supelco,&nbsp; &ge; 99,5% (HPLC)</td><td>92817-1G</td><td>9-Aminoacridina matrice sostanza per MALDI-MS; https://www.sigmaaldrich.com/US/en/product/sial/92817?srsltid=AfmBOooiQjQ4pWv_XxITkU 4Lkm0UnHXKekGS_ dFl7V40V9QLWoPpNLoc</td></tr><tr><td>Aquasonic Gel per ultrasuoni</td><td>Parker Laboratories</td><td>Parker 01-02</td><td>Gel per ultrasuoni; https://www.parkerlabs.com/products/aquasonic-100-ultrasound-transmission-gel/</td></tr> <tr><td>Flaconi Dewar da banco</td><td>TermoScientifico&nbsp;</td><td>4150-2000</td><td>Contenitore per azoto liquido; https://www.thermofisher.com/order/catalog/product/4150-4000?gclid=Cj0KCQjwpvK4BhDUARIsA DHt9sQVc2f-NxN04Nb5Mv F6TZ7GLHWWDEeqDYmEvtKJSQ YHDeVgZ9qylvYaAs27EALw_wcB & fonte=google_shopping& ISO_ CODICE=us& LANG_CODE=it& ef_id =Cj0KCQjwpvK4BhDUARIsADHt9 sQVc2f-NxN04Nb5MvF6TZ7GLHWWDE eqDYmEvtKJSQYHDeVgZ9qylvYa As27EALw_wcB:G:s& s_kwcid=AL!3652 !3!716188292869!!! g!2366243726129 !! 21787513085!171591181194& ev_chn =negozio& cid=0se_gaw_30092024_ PBYTXL& fonte=google_shopping & ISO_CODE=noi& LANG_CODE= en& gad_source=1</td></tr><tr><td>Criostato</td><td>Leica Biosystems</td><td>Serie CM</td><td>https://www.leicabiosystems.com/us/histology-equipment/cryostats/</td></tr> <tr><td>Essiccatore</td><td>VWR</td><td>89054-052</td><td>https://us.vwr.com/store/product/9104882/desiccator-plastic-ace-glass-incorporated</td></tr><tr><td>Epredia MX35 Premier monouso a basso profilo&nbsp; Microtomo&nbsp; Lame</td><td>Fisher Scientific</td><td>3052835</td><td>Lama per criostato; https://www.fishersci.com/shop/products/mx35-premier-disposable-low-profile-microtome-blades/3052835</td></tr><tr><td>Falcon 15 mL Provette coniche per centrifuga</td><td>Fisher Scientific</td><td>14-959-53A</td><td>Provette coniche; https://www.fishersci.com/shop/products/falcon-15ml-conical-centrifuge-tubes-5/1495953A?gclid=Cj0KCQjwpvK4BhDUARIsA DHt9sSBcy5n-lhShligJUOX5KKVGn0bt87 8AB2_muOD2PPTue1phpZgeqwa AqgiEALw_wcB& ef_id=Cj0KCQjw pvK4BhDUARIsADHt9sSBcy5n-lhS hligJUOX5KKVGn0bt878AB2_muO D2PPTue1phpZgeqwaAqgiEALw_ wcB:G:s& ppc_id=PLA_goog_20861 45674_81843405034_1495953A__ 386247001345_165426395473886 37329& ev_chn=negozio& s_kwcid=AL!4428!3 !386247001345!!! g!856907751004!& gad_source=1</td></tr><tr><td>Provette per microcentrifuga Flex-Tubes</td><td>Eppendorf&nbsp;</td><td>EP022364120</td><td>Provette per centrifuga; https://www.sigmaaldrich.com/US/en/product/sigma/ep022364120?utm_source=google%2Cgoogle&utm _medium=organicshopping%2Ccpc& utm _campaign=21043330280& utm_ content=& gclid=Cj0KCQjwpv K4BhDUARIsADHt9sTHRD35k CHPtfI2A41axodnMVr6a1eBKk zM4bSUYQAyfEKo3UgTAEQa Ap7wEALw_wcB</td></tr><tr><td>Gas Azoto</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Vetrini per microscopio</td><td>Microscopia elettronica</td><td>Scienze 71873-02</td><td>https://www.emsdiasum.com/positive-charge-microscope-slides</td></tr><tr><td>Azoto liquido</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Spettrometro di massa</td><td>Waters</td><td>Synapt G2-Si</td><td>https://www.waters.com/waters/en_US/SYNAPT-G2-Si-Mass-Spectrometry/nav.htm?locale=en_mkcid=1000251Foodety%3C/a%3E&cid=134740653&bcid= 134528734</td></tr> spruzzatore a <tr><td>matrice</td><td>HTX Technologies</td><td>M3+</td><td>https://www.htximaging.com/htx-m3-sprayer</td></tr><tr><td>Metanolo (HPLC), >99,9%</td><td>Fisher Chemical&nbsp;</td><td>A4524</td><td>Metanolo; https://www.fishersci.com/shop/products/methanol-hplc-fisher-chemical-9/A4524?crossRef=A4524#?keyword=A4524</td></tr><tr><td>Ecografo Preclinico&nbsp;</td><td>FUJIFILM VisualSonics</td><td>Vevo 3100</td><td>https://www.visualsonics.com/product/imaging-systems/vevo-3100; Vevo F2 ha sostituito il Vevo 3100 in produzione. Il sistema include un vaporizzatore di isoflurano e una scatola a induzione.</td></tr><tr><td>Reynolds Wrap</td><td>N/A</td><td>N/A</td><td>Foglio di alluminio</td></tr><tr><td>Signagel&nbsp; Gel per elettrodi</td><td>Parker Laboratories</td><td>Parker 15-60</td><td>Gel conduttore di elettrodi; https://www.parkerlabs.com/products/signagel-electrode-gel/</td></tr> <tr><td>Gelatina lubrificante sterile</td><td>Medline&nbsp;</td><td>MDS032273Z</td><td>Gel lubrificante; https://www.medline.com/ce/product/Sterile-Lubricating-Jelly/Lubricating-Jelly/Z05-PF03664?sku=MDPMDS032273H</td></tr><tr><td>Strumenti chirurgici: forbici, pinze/pinzette, suture Strumenti per</td><td>le scienze</td><td>11252-00, 11050-10, 14016-14, 14084-08, 15000-08</td><td>info@finescience.com</td></tr><tr><td>Spugne chirurgiche Confezione da 200 - Garze non sterili - Spugna per medicazione per la cura delle ferite di primo soccorso &ndash; Tessuto medico antiaderente, rete non aderente scrubbing</td><td>Medpride&nbsp;</td><td>B08RZGQ5GW</td><td>Garza; https://www.amazon.com/Medpride-Surgical-Sponges-200-Pack/dp/B08RZGQ5GW/ref=asc_df_B08RZGQ5GW/?tag=hyprod-20&linkCode=df0&h vadid=693270340506& hvpos= & hvnetw=g& HVRand=960915122 2290977669& hvpone=& HVPTWO= & hvqmt=& hvdev=c& hvdvcmdl=& hvlocint=& hvlocphy=9192978& HV TARGID=PLA-1245491514869& psc= 1& mcid=33f4d647c88630c79116 888d565a63b0</td></tr><tr><td>Composto Tissue-Plus O.C.T.&nbsp;</td><td>Fisher Scientific</td><td>23-730-571</td><td>OCT; https://www.fishersci.com/shop/products/tissue-plus-o-c-t-compound-2/23730571</td></tr><tr><td>Tamponi di cotone e applicatori con manico in legno</td><td>Fisherbrand</td><td>22-363-160</td><td>Batuffolo di cotone; https://www.fishersci.com/shop/products/wood-handled-cotton-swabs-applicators-8/p-7146852</td></tr></tbody></table>

studio multimodale del rimodellamento cardiovascolare murino: ecografia quadridimensionale e spettrometria di massa

Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging

I protocolli di imaging sopra descritti sono stati utilizzati per due studi preliminari: rimodellamento dell'infarto del miocardio (IM) e invecchiamento vascolare. Per gli esperimenti cardiaci, è stato eseguito un intervento chirurgico permanente di legatura dell'arteria coronarica al fine di indurre l'infarto miocardico acuto18,19. L'ecografia 4D e la MALDI MSI sono state eseguite progressivamente sullo stesso tessuto, svelando cambiamenti fisiologici e molecola...

Studio multimodale del rimodellamento cardiovascolare murino: ecografia quadridimensionale e spettrometria di massa

Cardiovascular disease (CVD) is the leading cause of death in the United States. Damage in&#160;the cardiovascular system can be due to environmental ...

multimodal-study-murine-cardiovascular-remodeling-four-dimensional

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JoVE Journal

Bioengineering

Bioingegneria

Questo video dimostra l'uso di un rotaie ad alta frequenza sonda ad ultrasuoni per eseguire ecocardiografia di un mouse anestetizzato. I metodi di descrivere sia le misure convenzionali bidimensionale e M-mode della funzione cardiaca, oltre alle nuove, gli strumenti più potenti come il color Doppler, analisi ceppo, così come l'imaging contrasto generale e mirata.

L&#39;ecocardiografia murini e imaging a ultrasuoni

L'ecocardiografia è comunemente usata per caratterizzare e quantificare in modo non invasivo i cambiamenti nella struttura e nella funzione cardiaca. Descriviamo un algoritmo di imaging basato su ultrasuoni che offre una misura surrogata migliorata della microstruttura miocardico e può essere eseguito utilizzando un software di analisi delle immagini ad accesso aperto.

Valutazione ad ultrasuoni della microstruttura miocardica

L'obiettivo di questo protocollo è quello di valutare in modo non invasivo i cambiamenti strutturali e funzionali cardiaci in un modello murino della malattia di cuore creato da trasversali costrizione aortica, con B e M-mode ecocardiografia e colore / impulsi di imaging onda Doppler.

Valutazione del Cardiac morfologiche e funzionali Variazioni modello murino di trasversale aortica costrizione da parte ecocardiografica Imaging

Questo protocollo fornisce una descrizione dettagliata dell'approccio ecocardiografico per la fenotipizzazione completa della funzione cardiaca e valvolare nei topi.

Approcci ecocardiografici e protocolli per completa caratterizzazione fenotipica di malattia valvolare cardiaca nei topi

Formazione immagine di ultrasuono è diventata una modalità comune per determinare le dimensioni luminale degli aneurismi dell'aorta toracici ed addominale in topi. Questo protocollo descrive la procedura per acquisire immagini affidabili e riproducibili ecografia bidimensionale dell'aorta ascendente ed addominale in topi.

Formazione immagine di ultrasuono dell'Aorta toracica ed addominale in topi per determinare le dimensioni di Aneurysm

Questo studio descrive un protocollo completo di risonanza magnetica cardiovascolare (CMR) per quantificare i parametri funzionali ventricolari sinistri del cuore del topo. Il protocollo descrive l'acquisizione, la post-elaborazione e l'analisi delle immagini CMR, nonché la valutazione di diversi parametri funzionali cardiaci.

Quantificazione della funzione ventricolare sinistra del cuore di topo, del ceppo miocardico e delle forze emodinamiche mediante risonanza magnetica cardiovascolare

Questo protocollo dimostra l'ecografia robotica (US) come un'alternativa pratica, economica e rapida alle tradizionali modalità di immagine non invasive.

Uso di ultrasuoni robotici 3D per l'analisi in vivo dei reni di topo

Riportiamo un metodo per la ricostruzione mesoscopica dell'intero cuore del topo combinando nuovi progressi nella trasformazione e colorazione dei tessuti con lo sviluppo di un microscopio a foglio di luce a scansione assiale.

Imaging ottico mesoscopico dell'intero cuore del topo

Il protocollo utilizza la microscopia avanzata a foglio di luce insieme a metodi di pulizia dei tessuti adattati per studiare strutture cardiache complesse nei cuori dei roditori, con un grande potenziale per la comprensione della morfogenesi e del rimodellamento cardiaco.

Imaging a foglio luminoso per rivelare la struttura cardiaca nei cuori dei roditori

L'ecografia Doppler ultraveloce (UFUS) con elevata risoluzione spaziale (100 μm) e sensibilità rappresenta una modalità di imaging non invasiva adatta per ottenere una panoramica qualitativa completa della vascolarizzazione epatica. L'UFUS facilita anche le misurazioni quantitative della microvascolarizzazione, con l'obiettivo di migliorare la nostra comprensione dei meccanismi delle malattie vascolari.

Imaging e quantificazione della vascolarizzazione epatica dei topi mediante ecografia Doppler ultraveloce

Scopri l'imaging ecografico 4D in vivo e la spettrometria di massa per studiare il rimodellamento cardiovascolare murino. Questo approccio multimodale consente di analizzare le alterazioni biomeccaniche e biomolecolari nel sistema cardiovascolare.

Studio multimodale del rimodellamento cardiovascolare murino: ecografia quadridimensionale e spettrometria di massa

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