Name: Modello 2.5D per la caratterizzazione meccanica ex vivo dellangiogenesi da germogliamento in tessuto vivente (Video) | JoVE Journal
Uploaded: 2025-02-28T13:10:00.000+00:00
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Description: Scopri un modello 2.5D ex vivo per caratterizzare meccanicamente l'angiogenesi da germogliamento in tessuto vivente. Questo metodo combina microscopia di forza di trazione con arterie carotidee suine per studiare le interazioni cellulari leader-follower.

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<meta charset="utf8"></head><body>Modello 2.5D versatile per l'analisi della germinazione cellulare nelle arterie suine

<ol><li>Carmeliet, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Angiogenesis+in+health+and+disease%5BTitle%5D)+AND+%22Nat+Med%22%5BJournal%5D)">Angiogenesis in health and disease.</a> Nat Med. 9 (6), 653-660 (2003).</li>
<li>Folkman, J. Angiogenesis in cancer, vascular, rheumatoid and other disease. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aFolkman%2C+J+%22Nat+Med%22">Nat Med</a>. 1, 27-30 (1995).</li>
<li>Kretschmer, M., Rüdiger, D., Zahler, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanical+aspects+of+angiogenesis%5BTitle%5D)+AND+%22Cancers%22%5BJournal%5D)">Mechanical aspects of angiogenesis.</a> Cancers. 13 (19), 4987(2021).</li>
<li>Bordeleau, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrix+stiffening+promotes+a+tumor+vasculature+phenotype%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Matrix stiffening promotes a tumor vasculature phenotype.</a> Proc Natl Acad Sci U S A. 114 (3), 492-497 (2017).</li>
<li>Blanco, R., Gerhardt, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((VEGF+and+Notch+in+tip+and+stalk+cell+selection%5BTitle%5D)+AND+%22Cold+Spring+Harb+Perspect+Med%22%5BJournal%5D)">VEGF and Notch in tip and stalk cell selection.</a> Cold Spring Harb Perspect Med. 3 (1), a006569(2013).</li>
<li>Adams, R. H., Alitalo, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Molecular+regulation+of+angiogenesis+and+lymphangiogenesis%5BTitle%5D)+AND+%22Nat+Rev+Mol+Cell+Biol%22%5BJournal%5D)">Molecular regulation of angiogenesis and lymphangiogenesis.</a> Nat Rev Mol Cell Biol. 8, 464-478 (2007).</li>
<li>Carmeliet, P., De Smet, F., Loges, S., Mazzone, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Branching+morphogenesis+and+antiangiogenesis+candidates%3A+tip+cells+lead+the+way%5BTitle%5D)+AND+%22Nat+Rev+Clin+Oncol%22%5BJournal%5D)">Branching morphogenesis and antiangiogenesis candidates: tip cells lead the way.</a> Nat Rev Clin Oncol. 6, 315-326 (2009).</li>
<li>Salam, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+migration+of+human+mscs+through+fibrin+hydrogels+as+a+tool+for+formulation+optimisation%5BTitle%5D)+AND+%22Materials%22%5BJournal%5D)">Assessment of migration of human mscs through fibrin hydrogels as a tool for formulation optimisation.</a> Materials. 11 (9), 1781(2018).</li>
<li>Solbu, A. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+cell+migration+in+hydrogels%3A+An+overview+of+relevant+materials+and+methods%5BTitle%5D)+AND+%22Materials+Today+Bio%22%5BJournal%5D)">Assessing cell migration in hydrogels: An overview of relevant materials and methods.</a> Materials Today Bio. 18, 100537(2023).</li>
<li>Cao, W., Li, X., Zuo, X., Gao, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Migration+of+endothelial+cells+into+photo-responsive+hydrogels+with+tunable+modulus+under+the+presence+of+pro-inflammatory+macrophages%5BTitle%5D)+AND+%22Regenerat+Biomater%22%5BJournal%5D)">Migration of endothelial cells into photo-responsive hydrogels with tunable modulus under the presence of pro-inflammatory macrophages.</a> Regenerat Biomater. 6 (5), 259-267 (2019).</li>
<li>Staton, C. A., Reed, M. W. R., Brown, N. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+critical+analysis+of+current+in+vitro+and+in+vivo+angiogenesis+assays%5BTitle%5D)+AND+%22Int+J+Exp+Pathol%22%5BJournal%5D)">A critical analysis of current in vitro and in vivo angiogenesis assays.</a> Int J Exp Pathol. 90 (3), 195-221 (2009).</li>
<li>Staton, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Current+methods+for+assaying+angiogenesis+in+vitro+and+in+vivo%5BTitle%5D)+AND+%22Int+J+Exp+Path%22%5BJournal%5D)">Current methods for assaying angiogenesis in vitro and in vivo.</a> Int J Exp Path. 85, 233-248 (2004).</li>
<li>Ngo, T. X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+Vitro+models+for+angiogenesis+research%3A+A+review%5BTitle%5D)+AND+%22Int+J+Tissue+Regenerat%22%5BJournal%5D)">In Vitro models for angiogenesis research: A review.</a> Int J Tissue Regenerat. 5, 37-45 (2014).</li>
<li>Tomita, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+ex+vivo+choroid+sprouting+assay+of+ocular+microvascular+angiogenesis%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">An ex vivo choroid sprouting assay of ocular microvascular angiogenesis.</a> J Vis Exp. (162), e61677(2020).</li>
<li>Kapoor, A., Chen, C. G., Iozzo, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simplified+aortic+ring+assay%3A+A+useful+ex+vivo+method+to+assess+biochemical+and+functional+parameters+of+angiogenesis%5BTitle%5D)+AND+%22Matrix+Biol+Plus%22%5BJournal%5D)">A simplified aortic ring assay: A useful ex vivo method to assess biochemical and functional parameters of angiogenesis.</a> Matrix Biol Plus. 6-7, 100025(2020).</li>
<li>Stiffey-Wilusz, J., Boice, J. A., Ronan, J., Fletcher, A. M., Anderson, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+ex+vivo+angiogenesis+assay+utilizing+commercial+porcine+carotid+artery%3A+Modification+of+the+rat+aortic+ring+assay%5BTitle%5D)+AND+%22Angiogenesis%22%5BJournal%5D)">An ex vivo angiogenesis assay utilizing commercial porcine carotid artery: Modification of the rat aortic ring assay.</a> Angiogenesis. 4 (1), 3-9 (2001).</li>
<li>Kniazeva, E., Putnam, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Endothelial+cell+traction+and+ECM+density+influence+both+capillary+morphogenesis+and+maintenance+in+3-D%5BTitle%5D)+AND+%22Am+J+Physiol+Cell+Physiol%22%5BJournal%5D)">Endothelial cell traction and ECM density influence both capillary morphogenesis and maintenance in 3-D.</a> Am J Physiol Cell Physiol. 297 (1), C179-C187 (2009).</li>
<li>Davidson, C. D., Wang, W. Y., Zaimi, I., Jayco, D. K. P., Baker, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cell+force-mediated+matrix+reorganization+underlies+multicellular+network+assembly%5BTitle%5D)+AND+%22Sc+Rep%22%5BJournal%5D)">Cell force-mediated matrix reorganization underlies multicellular network assembly.</a> Sc Rep. 9 (1), 12(2019).</li>
<li>Lyle, K. S., Corleto, J. A., Wittmann, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microtubule+dynamics+regulation+contributes+to+endothelial+morphogenesis%5BTitle%5D)+AND+%22BioArchitecture%22%5BJournal%5D)">Microtubule dynamics regulation contributes to endothelial morphogenesis.</a> BioArchitecture. 2 (6), 220-227 (2012).</li>
<li>Kniazeva, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantification+of+local+matrix+deformations+and+mechanical+properties+during+capillary+morphogenesis+in+3D%5BTitle%5D)+AND+%22Integrat+Biol%22%5BJournal%5D)">Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D.</a> Integrat Biol. 4 (4), 431-439 (2012).</li>
<li>Quintanilla, M. A., Hammer, J. A., Beach, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-muscle+myosin+2+at+a+glance%5BTitle%5D)+AND+%22J+Cell+Sci%22%5BJournal%5D)">Non-muscle myosin 2 at a glance.</a> J Cell Sci. 136 (5), jcs.260890(2023).</li>
<li>Fischer, R. S., Gardel, M., Ma, X., Adelstein, R. S., Waterman, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Local+cortical+tension+by+myosin+II+guides+3D+endothelial+cell+branching%5BTitle%5D)+AND+%22Curr+Biol%22%5BJournal%5D)">Local cortical tension by myosin II guides 3D endothelial cell branching.</a> Curr Biol. 19 (3), 260-265 (2009).</li>
<li>Yoon, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myosin+IIA%E2%80%93mediated+forces+regulate+multicellular+integrity+during+vascular+sprouting%5BTitle%5D)+AND+%22Mol+Biol+Cell%22%5BJournal%5D)">Myosin IIA–mediated forces regulate multicellular integrity during vascular sprouting.</a> Mol Biol Cell. 30 (16), 1974-1984 (2019).</li>
<li>Du, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+characterization+of+mechanical+interactions+between+endothelial+cells+and+extracellular+matrix+during+angiogenic+sprouting%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Three-dimensional characterization of mechanical interactions between endothelial cells and extracellular matrix during angiogenic sprouting.</a> Sci Rep. 6, 21362(2016).</li>
<li>Vaeyens, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrix+deformations+around+angiogenic+sprouts+correlate+to+sprout+dynamics+and+suggest+pulling+activity%5BTitle%5D)+AND+%22Angiogenesis%22%5BJournal%5D)">Matrix deformations around angiogenic sprouts correlate to sprout dynamics and suggest pulling activity.</a> Angiogenesis. 23 (3), 315-324 (2020).</li>
<li>Style, R. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traction+force+microscopy+in+physics+and+biology%5BTitle%5D)+AND+%22Soft+Matter%22%5BJournal%5D)">Traction force microscopy in physics and biology.</a> Soft Matter. 10 (23), 4047-4055 (2014).</li>
<li>Trepat, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Physical+forces+during+collective+cell+migration%5BTitle%5D)+AND+%22Nat+Phys%22%5BJournal%5D)">Physical forces during collective cell migration.</a> Nat Phys. 5 (6), 426-430 (2009).</li>
<li>Santos-Oliveira, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+force+at+the+tip+-+modelling+tension+and+proliferation+in+sprouting+angiogenesis%5BTitle%5D)+AND+%22PLoS+Comput+Biol%22%5BJournal%5D)">The force at the tip - modelling tension and proliferation in sprouting angiogenesis.</a> PLoS Comput Biol. 11 (8), e1004436(2015).</li>
<li>Boreddy, S. R., Sahu, R. P., Srivastava, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Benzyl+isothiocyanate+suppresses+pancreatic+tumor+angiogenesis+and+invasion+by+inhibiting+HIF-%CE%B1%2FVEGF%2FRho-GTPases%3A+Pivotal+role+of+STAT-3%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Benzyl isothiocyanate suppresses pancreatic tumor angiogenesis and invasion by inhibiting HIF-α/VEGF/Rho-GTPases: Pivotal role of STAT-3.</a> PLoS One. 6 (10), 0025799(2011).</li>
<li>Teng, R. J., Eis, A., Bakhutashvili, I., Arul, N., Konduri, G. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Increased+superoxide+production+contributes+to+the+impaired+angiogenesis+of+fetal+pulmonary+arteries+with+in+utero+pulmonary+hypertension%5BTitle%5D)+AND+%22Am+J+Physiol+Lung+Cell+Mol+Physiol%22%5BJournal%5D)">Increased superoxide production contributes to the impaired angiogenesis of fetal pulmonary arteries with in utero pulmonary hypertension.</a> Am J Physiol Lung Cell Mol Physiol. 297 (1), L184-L195 (2009).</li>
<li>Costa, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Asymmetric+division+coordinates+collective+cell+migration+in+angiogenesis%5BTitle%5D)+AND+%22Nature+Cell+Biol%22%5BJournal%5D)">Asymmetric division coordinates collective cell migration in angiogenesis.</a> Nature Cell Biol. 18 (12), 1292-1301 (2016).</li>
<li>Slater, K., Partridge, J., Nandivada, H. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSlater%2C+K+%22Corning+tuning+the+elastic+moduli+of+Corning+Matrigel+and+collagen+I+3D+matrices+by+varying+the+protein+concentration%22">Corning tuning the elastic moduli of Corning Matrigel and collagen I 3D matrices by varying the protein concentration</a>. , Corning Incorporated, Life Sciences, MA USA. (2019).</li>
<li>Lee, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+chain+flexibility+on+cell+adhesion%3A+Semi-flexible+model-based+analysis+of+cell+adhesion+to+hydrogels%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Effect of chain flexibility on cell adhesion: Semi-flexible model-based analysis of cell adhesion to hydrogels.</a> Sci Rep. 9 (1), 2463(2019).</li>
<li>Motte, S., Kaufman, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Strain+stiffening+in+collagen+i+networks%5BTitle%5D)+AND+%22Biopolymers%22%5BJournal%5D)">Strain stiffening in collagen i networks.</a> Biopolymers. 99 (1), 35-46 (2013).</li>
<li>Butcher, D. T., Alliston, T., Weaver, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+tense+situation%3A+Forcing+tumour+progression%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">A tense situation: Forcing tumour progression.</a> Nat Rev Cancer. 9 (2), 108-122 (2009).</li>
<li>Artym, V. V., Matsumoto, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Imaging+cells+in+three-dimensional+collagen+matrix%5BTitle%5D)+AND+%22Curr+Prot+Cell+Biol%22%5BJournal%5D)">Imaging cells in three-dimensional collagen matrix.</a> Curr Prot Cell Biol. 10, Unit 10.18.1-Unit 10.18.20 (2010).</li>
<li>Labernadie, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+mechanically+active+heterotypic+E-cadherin%2FN-cadherin+adhesion+enables+fibroblasts+to+drive+cancer+cell+invasion%5BTitle%5D)+AND+%22Nat+Cell+Biol%22%5BJournal%5D)">A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion.</a> Nat Cell Biol. 19 (3), 224-237 (2017).</li>
<li>Bazellières, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Control+of+cell-cell+forces+and+collective+cell+dynamics+by+the+intercellular+adhesome%5BTitle%5D)+AND+%22Nat+Cell+Biol%22%5BJournal%5D)">Control of cell-cell forces and collective cell dynamics by the intercellular adhesome.</a> Nat Cell Biol. 17 (4), 409-420 (2015).</li>
<li>Uroz, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traction+forces+at+the+cytokinetic+ring+regulate+cell+division+and+polyploidy+in+the+migrating+zebrafish+epicardium%5BTitle%5D)+AND+%22Nat+Mater%22%5BJournal%5D)">Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium.</a> Nat Mater. 18 (9), 1015-1023 (2019).</li>
<li>Dong, C., Nastaran, Z., Konstantopoulos, K. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aDong%2C+C+%22Biomechanics+in+oncology%22">Biomechanics in oncology</a>. , Springer Nature. Switzerland, AG. (2018).</li>
<li>Schwarz, U. S., Soiné, J. R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traction+force+microscopy+on+soft+elastic+substrates%3A+A+guide+to+recent+computational+advances%5BTitle%5D)+AND+%22Biochim+Biophys+Acta%22%5BJournal%5D)">Traction force microscopy on soft elastic substrates: A guide to recent computational advances.</a> Biochim Biophys Acta. 1853 (11), 3095-3104 (2015).</li>
<li>Toyjanova, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+Viscoelastic+traction+force+microscopy%5BTitle%5D)+AND+%22Soft+Matter%22%5BJournal%5D)">3D Viscoelastic traction force microscopy.</a> Soft Matter. 10 (40), 8095-8106 (2014).</li>
<li>Legant, W. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multidimensional+traction+force+microscopy+reveals+out-of-plane+rotational+moments+about+focal+adhesions%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Multidimensional traction force microscopy reveals out-of-plane rotational moments about focal adhesions.</a> Proc Natl Acad Sci U S A. 110 (3), 881-886 (2013).</li>
<li>Steinwachs, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+force+microscopy+of+cells+in+biopolymer+networks%5BTitle%5D)+AND+%22Nat+Meth%22%5BJournal%5D)">Three-dimensional force microscopy of cells in biopolymer networks.</a> Nat Meth. 13 (2), 171-176 (2016).</li>
<li>Franck, C., Maskarinec, S. A., Tirrell, D. A., Ravichandran, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+traction+force+microscopy%3A+A+new+tool+for+quantifying+cell-matrix+interactions%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Three-dimensional traction force microscopy: A new tool for quantifying cell-matrix interactions.</a> PLoS One. 6 (3), e17833(2011).</li>
</ol>

<table><tbody><tr><td>2% bis-acrilammide</td><td></td><td>Bio-Rad</td><td>1610143</td></tr><tr><td>2-mercaptoetanolo</td><td></td><td>Merck Life Science</td><td>60-24-2</td></tr><tr><td>3-(Trimetossisilil)metacrilato di propile</td><td>Legante-Silano</td><td>Sigma-Aldrich</td><td>440159-100ML</td></tr><tr><td>40% acrilammide</td><td></td><td>Bio-Rad</td><td>1610140</td></tr><tr><td>Aboslute etanolo (per analisi)</td><td></td><td>VWR International</td><td>1.00983.1000</td></tr><tr><td>Etanolo assoluto (industriale)</td><td></td><td>VWR International</td><td>83813.41</td></tr><tr><td>Acido acetico, glaciale 100%</td><td></td><td>Merck</td><td>1000562500</td></tr><tr><td>Persolfato di ammonio</td><td>APS</td><td>Bio-Rad</td><td>7727-54-0</td></tr><tr><td>anticorpo (primario) - calponina</td><td></td><td>abcam</td><td>ab46794</td></tr><tr><td>anticorpo (primario) - CD31</td><td></td><td>Serotec</td><td>MCA1746</td></tr><tr><td>anticorpo (primario) - &alfa;-actina muscolare liscia</td><td>&alfa;SMA</td> Anticorpo <td>Dako</td><td>M0851</td></tr><tr><td>(secondario) - capra-anti-topo-IgG1 Alexa 488</td><td></td><td>Sonde molecolari</td><td>A21121</td></tr><tr><td>anticorpo (secondario) - capra-anti-topo-IgG2a Alexa 555</td><td></td><td>Sonde molecolari</td><td>A21137</td></tr><tr><td>anticorpo (secondario) - capra-anti-coniglio-IgG Alexa 555</td><td></td><td>Sonde molecolari</td><td>A21428</td></tr><tr><td>Autoclave</td><td></td><td>Astell</td><td></td></tr><tr><td>Cloruro di calcio diidrato</td><td>CaCl2</td><td>Calbiochem</td><td>208291-250GM</td></tr><tr><td>Collagene di tipo I, coda</td><td></td> di topo <td>Corning</td><td>354236</td></tr><tr><td>Collagene di tipo IV, placenta</td><td></td> umana <td>Merck Life Science</td><td>C5533-5MG</td></tr><tr>Medium ECG<td>Cell Growth Medium</td><td></td><td>Promocell</td><td>C-22111</td></tr><tr><td>Pinzetta a punta tonda con rivestimento in epossissica</td><td>pinzetta fine</td><td>Rubis Pinzette</td><td>E78144-2A</td></tr><tr><td>Pennarello fluorescente, rosso</td><td></td> scuro <td>Invitrogen</td><td>F8807</td></tr><tr><td>Vetrini coprioggetti, &Oslash; 13 mm, #1</td><td></td><td>Epredia</td><td>CB00130RA120MNZ0</td></tr><tr><td>Vetrini coprioggetti, &Oslash; 13 mm, #1.5</td><td></td><td>Epredia</td><td>CB00120RAC20MNZ0</td></tr><tr><td>Acido cloridrato, 25%</td><td>HCl</td><td>Merck</td><td>1.100316.1000</td></tr><tr><td>tampone Krebs-Henseleit</td><td>Microscopio Sigma-Aldrich</td><td>K3753</td>, software Leica Application Suite X, versione 3.5.7.23225 Microscopio Leica Microsystems, microscopio a epifluorescenza Leica DMi8 Leica Microsystems <td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Microscopio, Nikon Ti2 Eclipse</td><td></td><td>Microscopio Nikon</td><td></td></tr><tr><td>, software</td><td></td> NIS-Elements AR <td>Nikon</td><td></td></tr><tr><td>N,N,N',N',N'-tetrametiletano-1,2-diammina</td><td>TEMED</td><td>Merck Life Science</td><td>110-18-9</td></tr><tr><td>Flacone</td><td></td> Nalgene <td>Thermo Scientific</td><td>2187-0016</td></tr><tr><td>Ago, 21Gx1"</td><td></td><td>Henke Sass Wolf</td><td>HK4710008025</td></tr><tr><td>Siero normale, capra</td><td></td><td>Gibco</td><td>10098792</td></tr><tr><td>Papaverina cloridrato</td><td></td><td>Sigma</td><td>61-25-6</td></tr><tr><td>Penicillina/Streptomiocin (10 000 U/mL)</td><td>P/S</td><td>Gibco</td><td>15140163</td></tr><tr><td>Petri-dish, grande (145x20mm)</td><td></td><td>Greiner Bio-one</td><td>639160</td></tr><tr><td>Petri-dish, piccolo (60x15mm)</td><td></td><td>Greiner Bio-one</td><td>628160</td></tr><tr><td>Soluzione salina tamponata con fosfato</td><td>PBS</td><td>Sigma</td><td>P4417</td></tr><tr><td>Pluronic F-127</td><td></td><td>Merck Life Science</td><td>P2443-250G</td></tr><tr><td>Ago per puntura, punta</td><td></td> chiusa affilata <td>sconosciuto</td><td></td></tr><tr><td>Bisturi, n. 4</td><td></td><td>Swann-Morton</td><td></td></tr><tr><td>Carbonato acido di sodio</td><td>NaHCO3</td><td>VWR International</td><td>144-55-8</td></tr><tr><td>sulfosuccinimidil 6-(4'-azido-2'-nitrofenilammino)esanoato</td><td>Sulfo-SANPAH</td><td>Thermo Scientific</td><td>22589</td></tr><tr><td>Lama chirurgica, n. 20</td><td></td><td>Swann-Morton</td><td></td></tr><tr><td>Telo</td><td></td> per teli chirurgici <td>Foliodrape</td><td>2775001</td></tr><tr><td>Pinzetta</td><td></td> chirurgica <td>Lettix</td><td>400024</td></tr><tr><td>Triton X-100</td><td></td><td>Merck</td><td>9036-19-5</td></tr><tr><td>Lampada UV</td><td></td><td>Analytik Jena</td><td>95-0042-13&nbsp;</td></tr><tr><td>piastra a pozzetti, 96 pozzetti, fondo</td><td></td> F <td>Greiner Bio-one</td><td>655180</td></tr><tr><td>pozzetti, fondo in vetro 12 pozzetti</td><td></td><td>MatTek</td><td>P12G-0-14-F</td></tr></tbody></table>

modello 2.5d per la caratterizzazione meccanica ex vivo dell'angiogenesi da germogliamento in tessuto vivente

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Modello 2.5D per la caratterizzazione meccanica ex vivo dell'angiogenesi da germogliamento in tessuto vivente

Sprouting angiogenesis is the formation of new blood vessels from pre-existing vasculature and is of great importance for physiological such as tissue ...

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JoVE Journal

Biology

Biologia

I progenitori endoteliali derivati da cellule staminali pluripotenti indotte (iPSC-EP) hanno il potenziale di rivoluzionare i trattamenti delle malattie cardiovascolari e di consentire la creazione di modelli di malattie cardiovascolari più fedeli. Qui, vengono descritti l'incapsulamento di iPS-EP in microambienti tridimensionali di collagene (3D) e un'analisi quantitativa del potenziale vasculogenico di queste cellule.

Un modello 3D in vitro e una pipeline computazionale per quantificare il potenziale vasculogenico dei progenitori endoteliali derivati da iPSC

Questo metodo descrive la coltura delle cellule endoteliali derivate da iPSC come 40 micronavi 3D perfuse in una piattaforma microfluidica standardizzata. Questa piattaforma consente lo studio della germogliazione angiogenica basata su gradienti in 3D, tra cui l'anastomosis e la stabilizzazione dei germogli angiogenici in modo scalabile e ad alta produttività.

Analisi standardizzata e scalabile per studiare la germogliazione angiogenica 3D perfusa di cellule endoteliali derivate da iPSC in Vitro

Qui forniamo un chip microfluidico e un sistema microfluidico di circolazione automaticamente controllato e altamente efficiente che ricapitola il microambiente iniziale della neovascolarizzazione, consentendo alle cellule endoteliali (EC) di essere stimolate da elevate sollecitazioni di taglio luminali, livello fisiologico di flusso transendoteliale e distribuzione simultanea di vari fattori di crescita endoteliali vascolari (VEGF).

Modello microfluidico per imitare l&apos;evento iniziale di neovascolarizzazione

Questo test utilizza cellule staminali embrionali di topo differenziate in corpi embrioidi coltivati in gel di collagene 3D per analizzare i processi biologici che controllano l'angiogenesi germinativa in vitro. La tecnica può essere applicata per testare farmaci, modellare malattie e per studiare geni specifici nel contesto di delezioni embrionalmente letali.

In vitro Saggio tridimensionale di germinazione dell'angiogenesi utilizzando cellule staminali embrionali di topo per la modellazione di malattie vascolari e test farmacologici

Questo studio presenta il saggio bidimensionale (2D) di migrazione della ferita da graffio e il saggio tridimensionale (3D) di germinazione degli sferoidi, insieme ai rispettivi metodi di analisi a valle, tra cui l'estrazione dell'RNA e l'immunocitochimica, come saggi adatti per studiare l'angiogenesi in vitro.

Indagine sull'angiogenesi a livello funzionale e molecolare sfruttando il saggio di migrazione della ferita da graffio e il saggio di germinazione sferoidale

CW Christopher Hughes descrive l'utilità del suo sistema di coltura per lo studio dell'angiogenesi in vitro. Egli spiega l'importanza dei fibroblasti che secernono un critico, non ancora identificato, fattore solubile che permettono alle cellule endoteliali a formare navi in ​​quel ramo della cultura, la forma corretta lumen, e subiscono anastamosis.

Christopher Hughes: un modello in vitro per lo studio dell&#39;angiogenesi (Intervista)

Un nuovo sistema in vitro per caricare simultaneamente quattro tendini nella cultura è descritta.

Ex vivo Carico meccanico del tendine

Utilizzando Micro-Electro-Mechanical Systems (MEMS) per sviluppare strumenti diagnostici

La rigidità della matrice extracellulare influenza fortemente i comportamenti multipli di cellule aderenti. Matrice di rigidezza varia spazialmente in tutto un tessuto, e subisce modifiche nelle condizioni patologiche diverse. Qui sviluppare metodi per la caratterizzazione variazioni spaziali della rigidità nei tessuti del mouse normale e fibrotica del polmone mediante microscopia a forza atomica micropenetrazione.

Micro-Caratterizzazione meccanica del tessuto polmonare usando la microscopia a forza atomica

Qui, presentiamo un protocollo basato sulla rilevazione immunoistologica della proteina c-FOS, una tecnica classica utilizzata per l'identificazione di popolazioni neuronali coinvolte in specifiche risposte fisiologiche in vivo ed ex vivo.

La proteina C-FOS immunoistologiche Detection: uno strumento utile come indicatore di percorsi centrali coinvolti nelle risposte fisiologiche specifiche<em&gt; In Vivo</em&gt; e<em&gt; Ex Vivo</em

Scopri un modello 2.5D ex vivo per caratterizzare meccanicamente l'angiogenesi da germogliamento in tessuto vivente. Questo metodo combina microscopia di forza di trazione con arterie carotidee suine per studiare le interazioni cellulari leader-follower.

Modello 2.5D per la caratterizzazione meccanica ex vivo dell'angiogenesi da germogliamento in tessuto vivente

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