Name: Isolamento e coltura di cellule primarie di Müller retiniche da ratti Sprague-Dawley (SD) (Video) | JoVE Journal
Uploaded: 2025-06-17T14:20:00.000+00:00
Duration: 7 min 41 s
Description: Impara l'isolamento e la coltura di cellule di Müller retiniche primarie da ratti Sprague-Dawley neonati. Questo protocollo standardizzato garantisce elevata purezza cellulare per studi di neuroscienza e biologia retinica.

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<li>Too, L. K., Gracie, G., Hasic, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adult+human+retinal+M%C3%BCller+glia+display+distinct+peripheral+and+macular+expression+of+CD117+and+CD44+stem+cell-associated+proteins%5BTitle%5D)+AND+%22Acta+Histochem%22%5BJournal%5D)">Adult human retinal Müller glia display distinct peripheral and macular expression of CD117 and CD44 stem cell-associated proteins.</a> Acta Histochem. 119 (2), 142-149 (2017).</li>
<li>Bhatia, B., Jayaram, H., Singhal, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differences+between+the+neurogenic+and+proliferative+abilities+of+M%C3%BCller+glia+with+stem+cell+characteristics+and+the+ciliary+epithelium+from+the+adult+human+eye%5BTitle%5D)+AND+%22Exp+Eye+Res%22%5BJournal%5D)">Differences between the neurogenic and proliferative abilities of Müller glia with stem cell characteristics and the ciliary epithelium from the adult human eye.</a> Exp Eye Res. 93 (6), 852-861 (2011).</li>
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<li>Xu, Q. A., Boerkoel, P., Hirsch-Reinshagen, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((M%C3%BCller+cell+degeneration+and+microglial+dysfunction+in+the+Alzheimer%27s+retina%5BTitle%5D)+AND+%22Acta+Neuropathol+Commun%22%5BJournal%5D)">Müller cell degeneration and microglial dysfunction in the Alzheimer's retina.</a> Acta Neuropathol Commun. 10 (1), 145(2022).</li>
<li>Bunt-Milam, A. H., Saari, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunocytochemical+localization+of+two+retinoid-binding+proteins+in+vertebrate+retina%5BTitle%5D)+AND+%22J+Cell+Biol%22%5BJournal%5D)">Immunocytochemical localization of two retinoid-binding proteins in vertebrate retina.</a> J Cell Biol. 97 (3), 703-712 (1983).</li>
<li>Masri, R. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunohistochemistry+and+spatial+density+of+M%C3%BCller+cells+in+the+human+fovea%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Immunohistochemistry and spatial density of Müller cells in the human fovea.</a> Invest Ophthalmol Vis Sci. 66 (2), 46(2025).</li>
<li>Kolesnikov, A. V., Kiser, P. D., Palczewski, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Function+of+mammalian+M-cones+depends+on+the+level+of+CRALBP+in+M%C3%BCller+cells%5BTitle%5D)+AND+%22J+Gen+Physiol%22%5BJournal%5D)">Function of mammalian M-cones depends on the level of CRALBP in Müller cells.</a> J Gen Physiol. 153 (1), e202012675(2021).</li>
<li>Xue, Y., Shen, S. Q., Jui, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CRALBP+supports+the+mammalian+retinal+visual+cycle+and+cone+vision%5BTitle%5D)+AND+%22J+Clin+Invest%22%5BJournal%5D)">CRALBP supports the mammalian retinal visual cycle and cone vision.</a> J Clin Invest. 125 (2), 727-738 (2015).</li>
<li>Pereiro, X., Beriain, S., Rodriguez, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characteristics+of+whale+M%C3%BCller+glia+in+primary+and+immortalized+cultures%5BTitle%5D)+AND+%22Front+Neurosci%22%5BJournal%5D)">Characteristics of whale Müller glia in primary and immortalized cultures.</a> Front Neurosci. 16, 854278(2022).</li>
<li>Tran, T. L., Bek, T., Holm, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aquaporins+6-12+in+the+human+eye%5BTitle%5D)+AND+%22Acta+Ophthalmol%22%5BJournal%5D)">Aquaporins 6-12 in the human eye.</a> Acta Ophthalmol. 91 (6), 557-563 (2013).</li>
<li>Gusel'nikova, V. V., Korzhevskiy, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((NeuN+as+a+neuronal+nuclear+antigen+and+neuron+differentiation+marker%5BTitle%5D)+AND+%22Acta+Naturae%22%5BJournal%5D)">NeuN as a neuronal nuclear antigen and neuron differentiation marker.</a> Acta Naturae. 7 (2), 42-47 (2015).</li>
<li>Zeng, Q., Xia, X. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Study+on+the+differentiation+of+retinal+ganglion+cells+from+rat+M%C3%BCller+cells+in+vitro%5BTitle%5D)+AND+%22Yan+Ke+Za+Zhi%22%5BJournal%5D)">Study on the differentiation of retinal ganglion cells from rat Müller cells in vitro.</a> Yan Ke Za Zhi. 46 (7), 615-620 (2010).</li>
<li>Backstrom, J. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Phenotypes+of+primary+retinal+macroglia%3A+Implications+for+purification+and+culture+conditions%5BTitle%5D)+AND+%22Exp+Eye+Res%22%5BJournal%5D)">Phenotypes of primary retinal macroglia: Implications for purification and culture conditions.</a> Exp Eye Res. 182, 85-92 (2019).</li>
<li>Liu, X., Tang, L., Liu, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+M%C3%BCller+cell+isolation+and+culture%5BTitle%5D)+AND+%22Bio+Protoc%22%5BJournal%5D)">Mouse Müller cell isolation and culture.</a> Bio Protoc. 7 (15), e2429(2017).</li>
<li>Pereiro, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+a+method+to+isolate+and+culture+adult+porcine%2C+rats+and+mice+M%C3%BCller+glia+in+order+to+study+retinal+diseases%5BTitle%5D)+AND+%22Front+Cell+Neurosci%22%5BJournal%5D)">Optimization of a method to isolate and culture adult porcine, rats and mice Müller glia in order to study retinal diseases.</a> Front Cell Neurosci. 14 (7), (2020).</li>
<li>Wang, M., Ma, W., Zhao, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adaptive+M%C3%BCller+cell+responses+to+microglial+activation+mediate+neuroprotection+and+coordinate+inflammation+in+the+retina%5BTitle%5D)+AND+%22J+Neuroinflammation%22%5BJournal%5D)">Adaptive Müller cell responses to microglial activation mediate neuroprotection and coordinate inflammation in the retina.</a> J Neuroinflammation. 8, 173(2011).</li>
<li>Tomaszewski, R., Gad, M. S., Negoita, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+of+primary+mouse+retinal+glial+M%C3%BCller+cells%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Isolation of primary mouse retinal glial Müller cells.</a> J Vis Exp. (210), e66237(2024).</li>
<li>Li, Q., Cheng, Y., Zhang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((TRPV4-induced+M%C3%BCller+cell+gliosis+and+TNF-%CE%B1+elevation-mediated+retinal+ganglion+cell+apoptosis+in+glaucomatous+rats+via+JAK2%2FSTAT3%2FNF-%CE%BAB+pathway%5BTitle%5D)+AND+%22J+Neuroinflammation%22%5BJournal%5D)">TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway.</a> J Neuroinflammation. 18 (1), 271(2021).</li>
<li>McCarthy, K. D., de Vellis, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preparation+of+separate+astroglial+and+oligodendroglial+cell+cultures+from+rat+cerebral+tissue%5BTitle%5D)+AND+%22J+Cell+Biol%22%5BJournal%5D)">Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.</a> J Cell Biol. 85 (3), 890-902 (1980).</li>
<li>Reichenbach, A., Wolburg, H., Richter, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Membrane+ultrastructure+preservation+and+membrane+potentials+after+isolation+of+rabbit+retinal+glial+%28M%C3%BCller%29+cells+by+papain%5BTitle%5D)+AND+%22J+Neurosci+Methods%22%5BJournal%5D)">Membrane ultrastructure preservation and membrane potentials after isolation of rabbit retinal glial (Müller) cells by papain.</a> J Neurosci Methods. 32 (3), 227-233 (1990).</li>
<li>Harstad, H. K., Ringvold, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Scanning+and+transmission+electron+microscopy+of+M%C3%BCller+cells+isolated+from+rabbit+retina%5BTitle%5D)+AND+%22Graefes+Arch+Clin+Exp+Ophthalmol%22%5BJournal%5D)">Scanning and transmission electron microscopy of Müller cells isolated from rabbit retina.</a> Graefes Arch Clin Exp Ophthalmol. 223 (1), 29-34 (1985).</li>
<li>Guidry, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+and+characterization+of+porcine+M%C3%BCller+cells.+Myofibroblastic+dedifferentiation+in+culture%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Isolation and characterization of porcine Müller cells. Myofibroblastic dedifferentiation in culture.</a> Invest Ophthalmol Vis Sci. 37 (5), 740-752 (1996).</li>
<li>Qiu, A. W., Wang, N. Y., Yin, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Retinal+M%C3%BCller+cell-released+exosomal+miR-92a-3p+delivers+interleukin-17A+signal+by+targeting+Notch-1+to+promote+diabetic+retinopathy%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Retinal Müller cell-released exosomal miR-92a-3p delivers interleukin-17A signal by targeting Notch-1 to promote diabetic retinopathy.</a> Invest Ophthalmol Vis Sci. 66 (1), 1(2025).</li>
</ol>

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isolamento e coltura di cellule primarie di müller retiniche da ratti sprague-dawley (sd)

Isolation and Culture of Primary Retinal M&#252;ller Cells from Sprague-Dawley (SD) Rats

Dopo che le cellule primarie sono state seminate nel pallone di coltura, i successivi cambiamenti del terreno e il passaggio aiutano a eliminare le RMC scarsamente aderenti e a rimuovere i detriti cellulari generati durante il passaggio, arricchendo così la popolazione di RMC nella coltura. Gli RMC sono stati identificati in base alla loro morfologia utilizzando un microscopio ottico invertito (Figura 1) e la colorazione con ematossilina ed eosina (H&amp;E) (...

Isolamento e coltura di cellule primarie di Müller retiniche da ratti Sprague-Dawley (SD)

Retinal M&#252;ller cells (RMCs) play a crucial role in providing structural support and regulating various functions within the retina. As a core ...

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In questo video ci dimostra come isolare le cellule staminali della retina dalla epitelio ciliare dell'occhio del mouse e farle crescere in coltura per formare clonale sfere della retina. Le sfere che sono isolati possiedono le caratteristiche cardine delle cellule staminali: l'auto-rinnovamento e multipotenzialità.

Isolamento di cellule staminali della retina dalla Eye mouse

Milioni di persone soffrono di malattie degenerative della retina che provocano cecità irreversibile. Un elemento comune di molte di queste malattie è la perdita delle cellule del ganglio retinico (RGC). Questo protocollo dettagliato descrive l'isolamento di RGC murine primari mediante selezione positiva e negativa con citometria a flusso.

Isolamento delle cellule della ganglioma della retina primaria (RGC) mediante citometria a flusso

L'epitelio pigmentato retinico (RPE) è un multi-funzionale dell'epitelio dell'occhio. Qui presentiamo un protocollo standard per stabilire colture cellulari primarie derivate da RPE murino.

Colture cellulari primarie dall'epitelio retinico del pigmento del Mouse

Questo protocollo, originariamente riportato da Fernandez-Godino et al. La procedura dura circa 3 ore.

Dissezione e coltura efficienti delle cellule epiteliali del pigmento retino del topo primario

Questo manoscritto descrive un protocollo semplificato per l'isolamento delle cellule dell'epitelio pigmentato retinico (RPE) dagli occhi dei topi in modo graduale. Il protocollo include l'enucleazione e la dissezione degli occhi di topo, seguita dall'isolamento, dalla semina e dalla coltura delle cellule RPE.

Isolamento delle cellule primarie dell'epitelio pigmentato retinico del topo

Questo protocollo delinea la procedura per ottenere e coltivare cellule epiteliali pigmentate retiniche primarie (RPE) da occhi suini di provenienza locale. Queste cellule rappresentano un'alternativa di alta qualità alle cellule staminali e sono adatte per la ricerca retinica in vitro .

Isolamento di cellule epiteliali pigmentate retiniche primarie suine per la modellazione in vitro

Questo manoscritto descrive un protocollo dettagliato per isolare le cellule di Müller gliali retiniche dagli occhi di topo. Il protocollo inizia con l'enucleazione e la dissezione degli occhi di topo, seguita dall'isolamento, dalla semina e dalla coltura delle cellule di Müller.

Isolamento delle cellule primarie di Müller gliali retiniche di topo

L'epitelio pigmentato retinico (RPE) agisce come una barriera cruciale tra la coroide e la retina, promuovendo la salute e la funzione dei tipi di cellule retiniche, come i fotorecettori. In questo articolo, descriviamo un protocollo semplice ed efficace per isolare e coltivare l'EPR murino adulto.

Un metodo semplificato per l'isolamento e la coltura di cellule epiteliali pigmentate retiniche da topi adulti

La dissezione e la crescita delle cellule da una zona del cervello individuale facilita l'investigazione dei parametri cellulari e fisiologici. Descriviamo un metodo per la coltura di cellule primarie che produce neurone arricchito di culture in un ambiente privo di siero.

Cultura primaria di neuroni dell&#39;ippocampo da P0 ratti neonati

Epatociti primari costituirà un valido strumento per valutare le funzioni biochimiche, molecolari e metaboliche in un sistema fisiologicamente rilevante sperimentale. Noi descriviamo un protocollo affidabile per la perfusione di fegato di ratto in situ, che genera costantemente epatociti vitali fino to1.0 ×...

Isolamento e la coltura primaria di cellule epatiche di ratto

Impara l'isolamento e la coltura di cellule di Müller retiniche primarie da ratti Sprague-Dawley neonati. Questo protocollo standardizzato garantisce elevata purezza cellulare per studi di neuroscienza e biologia retinica.

Isolamento e coltura di cellule primarie di Müller retiniche da ratti Sprague-Dawley (SD)

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