Name: Misurazioni del battito cardiaco senza anestesia in zebrafish in movimento libero (Video) | JoVE Journal
Uploaded: 2025-04-18T14:40:00.000+00:00
Duration: 3 min 57 s
Description: Scopri un metodo non invasivo per misurare il battito cardiaco in zebrafish in movimento libero senza anestesia, utilizzando imaging a infrarossi e algoritmi di rete neurale.

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</ol>

<table><tbody><tr><td>Reagenti</td><td></td><td></td><td></td></tr><tr><td>Agarosi a bassa temperatura di fusione</td><td>Biozym</td><td>850111</td><td></td></tr><tr><td>Sale da cucina</td><td>Pegasus&nbsp;</td><td>N/A</td><td></td></tr><tr><td>Tricaina (3-etilaminobenzoato metilsulfonato)&nbsp;</td><td>Sigma-Aldrich</td><td>E10505</td><td>MS-222</td></tr><tr><td>Attrezzature</td><td></td><td></td><td></td></tr><tr><td>Base con asta</td><td>Altami</td><td>SM-U1</td><td></td></tr><tr><td>Obiettivo collimatore</td><td>JLLSCMGGX</td><td></td><td>Lunghezza focale 30 mm</td></tr><tr><td>Meccanismo di messa a fuoco</td><td>Altami</td><td>SM-12</td><td>D=76 mm</td></tr><tr><td>LED</td><td>Cree</td><td>TR-3535IR-3W</td><td></td></tr><tr><td>Obiettivo</td><td>SFK Security</td><td></td><td>C-Mount, F1.6, 1/3&rdquo;,</td></tr><tr><td>Fotocamera a infrarossi vicini</td><td>ToupTek</td><td>SWIR1300KMA</td><td></td></tr><tr><td>Pipetta Pasteur</td><td>PE-LD</td><td>149293</td><td></td></tr><tr><td>Piatto di Petri 35 x 15 mm&nbsp;</td><td>BD Falcon</td><td>351008</td><td></td></tr><tr><td>Forme in plastica</td><td>N/A</td><td>N/A</td><td>Realizzati tramite stampa 3D</td></tr><tr><td>Alimentatore</td><td>Unit-T</td><td>UTP3300TFL-II</td><td></td></tr><tr><td>Stage</td><td>N/A</td><td>N/A</td><td>Realizzati tramite stampa 3D</td></tr><tr><td>Cacciavite</td><td>ErichKrause</td><td>19145</td><td></td></tr><tr><td>Oggetto di prova</td><td>Wally Sky</td><td>MS-1-EB</td><td></td></tr><tr><td>Software</td><td></td><td></td><td></td></tr><tr><td>EfficientDet</td><td>N/A</td><td>N/A</td><td>https://github.com/rwightman/efficientdet-pytorch</td></tr><tr><td>Modello EfficientNet-b0</td><td>N/A</td><td>N/A</td><td>https://arxiv.org/abs/1905.11946</td></tr><tr><td>Google API Client</td><td>Google</td><td>N/A</td><td>Google API Client è una libreria client Python per le API basate su discovery di Google. https://github.com/googleapis/google-api-python-client</td></tr><tr><td>Hardware</td><td></td><td></td><td></td></tr><tr><td>Rete di attenzione multi-scala</td><td>N/A</td><td>N/A</td><td>https://arxiv.org/abs/2209.14145</td></tr><tr><td>NVIDIA DIGITS</td><td>NVIDIA</td><td>N/A</td><td>NVIDIA DIGITS è un wrapper per Caffe che fornisce un'interfaccia web grafica. https://developer.nvidia.com/digits</td></tr><tr><td>GPU NVIDIA</td><td>NVIDIA</td><td>N/A</td><td>Una GPU NVIDIA è necessaria poiché alcuni dei framework software di seguito non funzioneranno altrimenti. https://www.nvidia.com</td></tr><tr><td>OpenCV</td><td>Intel</td><td>N/A</td><td>OpenCV è una libreria per la visione artificiale. https://opencv.org</td></tr><tr><td>Python</td><td>Python Software Foundation</td><td>N/A</td><td>Python è un linguaggio di programmazione. https://www.python.org</td></tr></tbody></table>

misurazioni del battito cardiaco senza anestesia in zebrafish in movimento libero

Anesthesia-free Heartbeat Measurements in Freely Moving Zebrafish

Misurazioni del battito cardiaco senza anestesia in zebrafish in movimento libero

Zebrafish (Danio rerio) is a widely used model organism in physiological, pharmacological, and toxicological research due to its genetic similarity to ...

anesthesia-free-heartbeat-measurements-in-freely-moving-zebrafish

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JoVE Journal

Bioengineering

Bioingegneria

Abbiamo sviluppato un semi-automatico ottica Metodo di analisi Heartbeat (SoHa) per analizzare le registrazioni ottico ad alta velocità...

Semiautomatici Analisi Heartbeat ottica di piccoli cuori

Questo video mostra come eseguire le misurazioni dell'elettrocardiogramma (ECG o ECG), un test che misura l'attività elettrica del cuore, su un pesce zebra.

Elettrocardiografia (ECG) di zebrafish: un test minimamente invasivo per valutare la funzione cardiaca

Questo documento descrive come un zebrafish adulto può essere immobilizzata, intubato, e utilizzati per esperimenti di elettrofisiologia in vivo per permettere registrazioni e manipolazione di attività neurale in un animale intatto.

Registrazione elettrofisiologica nel cervello di intatto Zebrafish adulti

L'uso del pesce zebra per la ricerca cardiovascolare si sta espandendo verso la ricerca sui cuori adulti. Per queste applicazioni, l'isolamento rapido e semplice dei tessuti cardiaci è fondamentale per evitare modifiche post-mortem e per ottenere un numero adeguato di campioni. Qui, descriviamo un metodo rapido e riproducibile per sezionare i cuori di pesce zebra adulto.

Recupero di Zebrafish adulti cuori per applicazioni ad alta velocità effettiva

Qui, presentiamo un metodo affidabile, minimamente invasivo e conveniente per registrare e interpretare gli elettrocardiogrammi nel pesce zebra adulto anestesizzato vivo.

Elettrocarcardio in superficie in vivo per il pesce zebra adulto

Questo articolo descrive un metodo per montare fragili embrioni di pesce zebra per la microscopia confocale time-lapse estesa. Questo metodo a basso costo è facile da eseguire utilizzando normali piatti di microscopia con fondo di vetro per l'imaging su qualsiasi microscopio invertito. Il montaggio viene eseguito in strati di agarose a diverse concentrazioni.

Un metodo di montaggio a strati per la microscopia confocale Time-Lapse estesa di embrioni di pesci zebra interi

Descriviamo un protocollo per valutare la morfologia cardiaca e la funzione nel pesce zebra adulto utilizzando l'ecocardiografia ad alta frequenza. Il metodo consente la visualizzazione del cuore e la successiva quantificazione dei parametri funzionali, come la frequenza cardiaca (HR), l'uscita cardiaca (CO), il cambiamento dell'area frazionaria (FAC), la frazione di espulsione (EF) e la velocità di afflusso e deflusso del sangue.

Ecocardia ad ultrasuoni ad alta frequenza per valutare la funzione cardiaca del pesce zebra

Descriviamo strumenti ottimizzati per studiare il cuore di zebrafish in vivo con microscopia a fluorescenza a foglio leggero. In particolare, suggeriamo linee transgeniche cardiache luminose e presentiamo nuove delicate tecniche di incorporamento e immobilizzazione che evitano difetti dello sviluppo e cardiaci. Viene inoltre fornita una possibile pipeline di acquisizione e analisi dei dati adattata all'imaging cardiaco.

Microscopia light sheet di dinamiche cardiache veloci in embrioni zebrafish

Questo protocollo utilizza l'imaging a foglio luminoso per studiare la funzione contrattile cardiaca nelle larve di zebrafish e ottenere informazioni sulla meccanica cardiaca attraverso il monitoraggio cellulare e l'analisi interattiva.

Imaging a foglio luminoso 4D della contrazione cardiaca del pesce zebra

Lo studio introduce Zebra II, un sistema avanzato per l'acquisizione e l'analisi prolungata dell'ECG nel pesce zebra. Questo sistema è dotato di un sistema di perfusione indipendente ed elettrodi a punti multipli per la movimentazione di più pesci in ambienti controllati. Gli effetti acuti dell'amiodarone sono stati valutati e analizzati con il pesce zebra wild-type.

Zebra II come nuovo sistema per registrare segnali elettrofisiologici nel pesce zebra

Questo protocollo descrive un metodo di microscopia ottica a basso costo per valutare la morfologia e la funzione cardiaca negli embrioni di zebrafish, consentendo una valutazione riproducibile della cardiotossicità dello sviluppo senza la necessità di sistemi di imaging avanzati.

Imaging cardiaco funzionale in embrioni di zebrafish utilizzando microscopia standard e analisi video: applicazioni nella ricerca ambientale e biomedica

Scopri un metodo non invasivo per misurare il battito cardiaco in zebrafish in movimento libero senza anestesia, utilizzando imaging a infrarossi e algoritmi di rete neurale.

Misurazioni del battito cardiaco senza anestesia in zebrafish in movimento libero

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