Name: Campionamento ambientale di microbi fotosintetici e dei loro virus: dal campo al laboratorio (Video) | JoVE Journal
Uploaded: 2025-07-03T14:30:00.000+00:00
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Description: Scopri metodi di campionamento ambientale per isolare cianofagi e caratterizzare le dinamiche virus-ospite usando tecniche di sequenziamento ad alta portata, microscopia elettronica e bioinformatica. Questo approccio avanzato supporta lo sviluppo di modelli predittivi in virologia.

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<li>Grasso, C. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+review+of+cyanophage-host+relationships%3A+Highlighting+cyanophages+as+a+potential+cyanobacteria+control+strategy%5BTitle%5D)+AND+%22Toxins%22%5BJournal%5D)">A review of cyanophage-host relationships: Highlighting cyanophages as a potential cyanobacteria control strategy.</a> Toxins. 14 (6), 385(2022).</li>
<li>Srivastava, A., Gupta, N., Mishra, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cyanophages%3A+interacting+mechanism+and+evolutionary+significance%5BTitle%5D)+AND+%22Cyanobacteria%22%5BJournal%5D)">Cyanophages: interacting mechanism and evolutionary significance.</a> Cyanobacteria. , 255-282 (2024).</li>
<li>Cai, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Abundant+and+cosmopolitan+lineage+of+cyanopodoviruses+lacking+a+DNA+polymerase+gene%5BTitle%5D)+AND+%22ISME+J%22%5BJournal%5D)">Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene.</a> ISME J. 17 (2), 252-262 (2023).</li>
<li>Komárek, J., Kaštovský, J., Mareš, J., Johansen, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Taxonomic+classification+of+cyanoprokaryotes+%28cyanobacterial+genera%29+2014%2C+using+a+polyphasic+approach%5BTitle%5D)+AND+%22Preslia%22%5BJournal%5D)">Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach.</a> Preslia. 86 (4), 295-335 (2014).</li>
</ol>

<table><tbody><tr><td>Pompa di trasferimento in acciaio inossidabile da 1 HP&nbsp;</td><td>Utilitech, Charlotte, Carolina del Nord, Stati Uniti</td><td>N/A</td><td></td></tr><tr><td>Tubi da 1,5 ml</td><td>www.eppendorf.com</td><td>30123611</td><td></td></tr><tr><td>Concentratore di centrifuga NMWL Centricon Plus 70 da 10K</td><td>www.sigmaaldrich.com</td><td>UFC701008</td><td></td></tr><tr><td>250 mL di fiasche di Erlenmeyer sconcertate&nbsp;</td><td>www.sigmaaldrich.com</td><td>CLS4450250</td><td></td></tr><tr><td>Generatore inverter da 3800 watt&nbsp;</td><td>WEN Products, West Dundee, IL, Stati Uniti</td><td>WEN 56380i</td><td></td></tr><tr><td>3K NMWL Amicon 15 mL&nbsp;</td><td>www.sigmaaldrich.com</td><td>UFC800308</td><td></td></tr><tr><td>500 mL di matracci di Erlenmeyer sconcertati&nbsp;</td><td>www.sigmaaldrich.com</td><td>CLS4450500</td><td></td></tr><tr><td>Brocche d'acqua da 5 galloni&nbsp;</td><td>www.homedepot.com</td><td>N/A</td><td></td></tr><tr><td>80 piedi di lunghezza, 0,5 " diametro, nylon&nbsp;</td><td>https://facomex.mx/producto/combo/</td><td>N/A</td><td></td></tr><tr><td>Una console centrale a V profonda 26 piedi Sailfish 2660 con due motori fuoribordo Yamaha da 150 CV.</td><td>https://yamahaoutboards.com/</td><td>N/A</td><td></td></tr><tr><td>Acetato di ammonio (CH3CO2NH4)</td><td>www.sigmaaldrich.com</td><td>631618</td><td></td></tr><tr><td>Acido borico (H3BO3)</td><td>www.sigmaaldrich.com</td><td>10043353</td><td></td></tr><tr><td>Cloruro di calcio diidrato (CaCl2. 2H2O)</td><td>www.sigmaaldrich.com</td><td>10035048</td><td></td></tr><tr><td>Centrifuga 5920&nbsp; R</td><td>www.eppendorf.com</td><td>5948000107</td><td></td></tr><tr><td>Acido citrico (C6H8O7)</td><td>www.sigmaaldrich.com</td><td>77929</td><td></td></tr><tr><td>Nitrato di cobalto(II) esaidrato [Co(NO3)2.6H2O]</td><td>www.sigmaaldrich.com</td><td>10026229</td><td></td></tr><tr><td>Tubo flessibile in gomma resistente al collasso&nbsp;</td><td>swanhose.com</td><td>CELTF58050</td><td></td></tr><tr><td>Solfato di rame(II) pentaidrato (CuSO4.5H2O)</td><td>www.sigmaaldrich.com</td><td>7758998</td><td></td></tr><tr><td>Provette da centrifuga Corning da 50 mL</td><td>www.sigmaaldrich.com</td><td>CLS430291</td><td></td></tr><tr><td>Fosfato dipotassico (K2HPO4)</td><td>www.sigmaaldrich.com</td><td>7758114</td><td></td></tr><tr><td>Acido etilendiamminotetraacetico disodico (Na2EDTA)</td><td>www.sigmaaldrich.com</td><td>6381926</td><td></td></tr><tr><td>DS-11 FX+_Microvolume e spettrofotometro e florometro</td><td>www.denovix.com</td><td>N/A</td><td></td></tr><tr><td>Nastro adesivo&nbsp;</td><td>www.homedepot.com</td><td>N/A</td><td></td></tr><tr><td>Provette Eppendorf</td><td>www.sigmaaldrich.com</td><td>EP022364120</td><td></td></tr><tr><td>Etanolo</td><td>www.sigmaaldrich.com</td><td>64175</td><td></td></tr><tr><td>Acido etilendiamminotetraacetico (EDTA)</td><td>www.sigmaaldrich.com</td><td>60004</td><td></td></tr><tr><td>Citrato ferrico di ammonio (C6H8O7&middot; xFe3+&middot; yNH3)</td><td>www.sigmaaldrich.com</td><td>1185575</td><td></td></tr><tr><td>Tubi in PVC blu Flex-plus&nbsp;</td><td>www.carlonsales.com</td><td>12008-750</td><td></td></tr><tr><td>Innova S44i - Agitatore per incubatore impilabile</td><td>www.eppendorf.com</td><td>2231001081</td><td></td></tr><tr><td>LVEM5 Microscopia elettronica Microscope_Transmission elettronica da banco (TEM)</td><td>delongamerica.com</td><td>N/A</td><td></td></tr><tr><td>Solfato di magnesio eptaidrato (MgSO4.7 H2O)</td><td>www.sigmaaldrich.com</td><td>10034998</td><td></td></tr><tr><td>Cloruro di manganese (II) tetraidrato (MnCl2.4H2O)</td><td>www.sigmaaldrich.com</td><td>13446349</td><td></td></tr><tr><td>Ancora a fungo rivestita in vinile nero/30 libbre</td><td>www.homedepot.com</td><td>N/A</td><td></td></tr><tr><td>Piastre di Petri in polistirolo</td><td>www.sigmaaldrich.com</td><td>P5481</td><td></td></tr><tr><td>Tazza filtrante a membrana in polietersulfone (PES) con 0,22 mm</td><td>www.sigmaaldrich.com</td><td>Z358193-1CS</td><td></td></tr><tr><td>Coppa del filtro a membrana in polietersulfone (PES) con 0,45 mm</td><td>www.sigmaaldrich.com</td><td>Z370622-1CS</td><td></td></tr><tr><td>Potassio etil xantogenato (C2H5OCSSK)</td><td>www.sigmaaldrich.com</td><td>140896</td><td></td></tr><tr><td>Carbonato di sodio (Na2CO3)</td><td>www.sigmaaldrich.com</td><td>497198</td><td></td></tr><tr><td>Sodio dodecil solfato [CH3(CH2)11OSO3Na]</td><td>www.sigmaaldrich.com</td><td>151213</td><td></td></tr><tr><td>Molibdato di sodio diidrato (Na2MoO4.2H2O)</td><td>www.sigmaaldrich.com</td><td>10102406</td><td></td></tr><tr><td>Nitrato di sodio (NaNO3)</td><td>www.sigmaaldrich.com</td><td>7631994</td><td></td></tr><tr><td>Tris(idrossimetil)metil]-2-amminoetanosolfonico (TES) (C6H15NO6S)</td><td>www.sigmaaldrich.com</td><td>7365448</td><td></td></tr><tr><td>Triton X-100&nbsp;</td><td>www.sigmaaldrich.com</td><td>T8787</td><td></td></tr><tr><td>Trizma cloridrato (Tris-HCl)</td><td>www.sigmaaldrich.com</td><td>1185531</td><td></td></tr><tr><td>Pompa a vuoto/pressione&nbsp;</td><td>Cole-Parmer; Vernon Hills, IL, Stati Uniti</td><td>EW-79204-00</td><td></td></tr><tr><td>Tubi in vinile&nbsp;</td><td>www.homedepot.com</td><td>N/A</td><td></td></tr><tr><td>Solfato di zinco eptaidrato (ZnSO4.7H2O)</td><td>www.sigmaaldrich.com</td><td>7446200</td><td></td></tr></tbody></table>

campionamento ambientale di microbi fotosintetici e dei loro virus: dal campo al laboratorio

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

Campionamento ambientale di microbi fotosintetici e dei loro virus: dal campo al laboratorio

Environmental sampling of photosynthetic microorganisms and their viruses plays a critical role in understanding contemporary marine and freshwater ...

environmental-sampling-photosynthetic-microbes-their-viruses-from

Research

Ricerca

JoVE Journal

Biology

Biologia

Il tasso di turnover dei virus in sistemi marini e d'acqua dolce può essere stimato da una riduzione e tecnica ripetersi. I dati permettono ai ricercatori di dedurre i tassi di virus-mediata mortalità microbica nei sistemi acquatici.

Stimare i tassi di produzione del virus nei sistemi acquatici

Qui, presentiamo un protocollo completo per valutare la disponibilità di nutrienti organici e inorganici e l'abbondanza e la struttura delle comunità microbiche e virali in ambienti marini remoti.

Svelare i giocatori invisibili del Mare - Una guida del campo di Acqua Chimica e microbiologia marina

Qui, presentiamo approcci fenomici per la caratterizzazione funzionale di geni fagici putativi. Le tecniche includono un test sviluppato in grado di monitorare il metabolismo anabolico dell'ospite, le piastre per saggi multifenotipo (MAP), oltre al metodo consolidato della metabolomica, in grado di misurare gli effetti sul metabolismo catabolico.

Fenomica fagi: Approcci fisiologici per caratterizzare Novel virali Proteine

Comprendere la composizione biologica del particolato ambientale è importante per lo studio dei relativi impatti significativi sulla salute umana e la diffusione della malattia. Qui, abbiamo utilizzato tre tipi di metodi di campionamento di bioaerosol e un'analisi biologica di microbi nell'aria per meglio esplorare le comunità microbiche nell'aria in diverse condizioni ambientali.

Composizione e analisi della distribuzione di Bioaerosols in diverse condizioni ambientali

Un progetto di citizen science è stato progettato per reclutare residenti a San Diego per raccogliere campioni ambientali per SARS-CoV-2. È stata creata una piattaforma multilingue basata sul Web per l'invio dei dati utilizzando un'interfaccia di dispositivo mobile intuitiva. Un sistema di gestione delle informazioni di laboratorio ha facilitato la raccolta di migliaia di campioni geograficamente diversi con il monitoraggio dei risultati in tempo reale.

Tamponare l'ambiente urbano - Una pipeline per il campionamento e il rilevamento della SARS-CoV-2 dai bacini ambientali

Questa pubblicazione descrive la progettazione di fotobioreattori da laboratorio (PBR) con regimi di luce personalizzabili. La crescita di cianobatteri o microalghe, utilizzando il bicarbonato come fonte di carbonio, viene monitorata continuamente misurando la produzione volumetrica di ossigeno. Questi PBR facilitano confronti di crescita di laboratorio rapidi e replicati con un piccolo intervento dell'utente durante gli esperimenti.

Funzionamento di fotobioreattori da laboratorio con misurazioni di crescita online e regimi di luce personalizzabili

La concentrazione di virus da campioni di acqua ambientale e acque reflue è un compito impegnativo, svolto principalmente per l'identificazione e la quantificazione dei virus. Mentre diversi metodi di concentrazione del virus sono stati sviluppati e testati, dimostriamo qui l'efficacia dell'ultrafiltrazione e della flocculazione del latte scremato per i virus a RNA con diversi tipi di campioni.

Concentrazione di particelle virali da campioni di acqua ambientale e acque reflue mediante flocculazione e ultrafiltrazione del latte scremato

Forme affidabili e accurate di analisi del DNA dispiegabile sul campo sono fondamentali per molti settori. Questo articolo offre due metodi per l'analisi microbica ambientale sul campo: 1) l'isolamento del DNA microbico da campioni ambientali utilizzando un kit di estrazione biologica sul campo, e 2) l'analisi del DNA tramite l'unità qPCR di campo.

Analisi del DNA microbico sul campo utilizzando un kit di estrazione biologica sul campo e un'unità qPCR sul campo

Questo video illustra la tecnica per estrarre il DNA dalla specie di microbi residenti nel hindgut termite. La preparazione di un vetrino a fresco, che è utile per visualizzare la comunità microbica intestinale è anche illustrato, e un tour attraverso le specie di ambiente ricco di intestino è dato.

Estrazione del DNA da microbi intestinali della Termite (Zootermopsis Angusticollis) e visualizzare microbi intestinali

Questo video documenta i metodi per la raccolta di campioni di acqua marina costiera e il loro trattamento per varie applicazioni a valle compresa la concentrazione della biomassa, purificazione degli acidi nucleici, l'abbondanza delle cellule, nutriente e analisi gas tracciante.

Campionamento di acqua di mare e di raccolta

Scopri metodi di campionamento ambientale per isolare cianofagi e caratterizzare le dinamiche virus-ospite usando tecniche di sequenziamento ad alta portata, microscopia elettronica e bioinformatica. Questo approccio avanzato supporta lo sviluppo di modelli predittivi in virologia.

Campionamento ambientale di microbi fotosintetici e dei loro virus: dal campo al laboratorio

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