Name: Costruzione di profili coppia-velocità-potenza a carico costante (isotonico) e a velocità costante (isocinetica) in vivo per i flessori plantari di ratto (Video) | JoVE Journal
Uploaded: 2025-10-03T13:50:00.000+00:00
Duration: 7 min 44 s
Description: Impara a costruire profili coppia-velocità-potenza in vivo nei flessori plantari di ratto usando stimolazione muscolare transcutanea. Questo metodo non invasivo permette di studiare la funzione muscolare e gli effetti delle malattie.

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<li>Yuan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+atypical+%CE%B2-blocker+S-oxprenolol+reduces+cachexia+and+improves+survival+in+a+rat+cancer+cachexia+model%5BTitle%5D)+AND+%22J+Cachexia+Sarcopenia+Muscle%22%5BJournal%5D)">The atypical β-blocker S-oxprenolol reduces cachexia and improves survival in a rat cancer cachexia model.</a> J Cachexia Sarcopenia Muscle. 14 (1), 653-660 (2023).</li>
<li>Hinks, A., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+differences+in+the+loss+and+recovery+of+serial+sarcomere+number+following+disuse+atrophy+in+rats%5BTitle%5D)+AND+%22Skeletal+Muscle%22%5BJournal%5D)">Age-related differences in the loss and recovery of serial sarcomere number following disuse atrophy in rats.</a> Skeletal Muscle. 14 (1), 18(2024).</li>
<li>Hulst, J. B., Minamoto, V. B., Lim, M. B., Bremner, S. N., Ward, S. R., Lieber, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Systematic+test+of+neurotoxin+dose+and+volume+on+muscle+function+in+a+rat+model%5BTitle%5D)+AND+%22Muscle+Nerve%22%5BJournal%5D)">Systematic test of neurotoxin dose and volume on muscle function in a rat model.</a> Muscle Nerve. 49 (5), 709-715 (2014).</li>
<li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+neural+stem+cells+transplanted+during+the+sequelae+phase+alleviate+motor+deficits+in+a+rat+model+of+cerebral+palsy%5BTitle%5D)+AND+%22Cytotherapy%22%5BJournal%5D)">Human neural stem cells transplanted during the sequelae phase alleviate motor deficits in a rat model of cerebral palsy.</a> Cytotherapy. 26 (12), 1491-1504 (2024).</li>
<li>Baltgalvis, K. A., Call, J. A., Cochrane, G. D., Laker, R. C., Yan, Z., Lowe, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exercise+training+improves+plantar+flexor+muscle+function+in+mdx+mice%5BTitle%5D)+AND+%22Med+Sci+Sports+Exerc%22%5BJournal%5D)">Exercise training improves plantar flexor muscle function in mdx mice.</a> Med Sci Sports Exerc. 44 (9), 1671-1679 (2012).</li>
<li>Sam, M., Shah, S., Fridén, J., Milner, D. J., Capetanaki, Y., Lieber, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Desmin+knockout+muscles+generate+lower+stress+and+are+less+vulnerable+to+injury+compared+with+wild-type+muscles%5BTitle%5D)+AND+%22Am+J+Physiol+Cell+Physiol%22%5BJournal%5D)">Desmin knockout muscles generate lower stress and are less vulnerable to injury compared with wild-type muscles.</a> Am J Physiol Cell Physiol. 279 (4), C1116-C1122 (2000).</li>
<li>Mintz, E. L., Passipieri, J. A., Lovell, D. Y., Christ, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applications+of+in+vivo+functional+testing+of+the+rat+tibialis+anterior+for+evaluating+tissue+engineered+skeletal+muscle+repair%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Applications of in vivo functional testing of the rat tibialis anterior for evaluating tissue engineered skeletal muscle repair.</a> J Vis Exp. (116), e54487(2016).</li>
<li>Song, H., Nakazato, K., Nakajima, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+increased+excursion+of+the+ankle+on+the+severity+of+acute+eccentric+contraction-induced+strain+injury+in+the+gastrocnemius%3A+an+in+vivo+rat+study%5BTitle%5D)+AND+%22Am+J+Sports+Med%22%5BJournal%5D)">Effect of increased excursion of the ankle on the severity of acute eccentric contraction-induced strain injury in the gastrocnemius: an in vivo rat study.</a> Am J Sports Med. 32 (5), 1263-1269 (2004).</li>
<li>Hinks, A., Patterson, M. A., Njai, B. S., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+blunting+of+serial+sarcomerogenesis+and+mechanical+adaptations+following+4+weeks+of+maximal+eccentric+resistance+training%5BTitle%5D)+AND+%22J+Appl+Physiol%22%5BJournal%5D)">Age-related blunting of serial sarcomerogenesis and mechanical adaptations following 4 weeks of maximal eccentric resistance training.</a> J Appl Physiol. 136 (5), 1209-1225 (2024).</li>
<li>Hinks, A. A., Vlemmix, E., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Submaximal+eccentric+resistance+training+increases+serial+sarcomere+number+and+improves+dynamic+muscle+performance+in+old+rats%5BTitle%5D)+AND+%22Physiol+Rep%22%5BJournal%5D)">Submaximal eccentric resistance training increases serial sarcomere number and improves dynamic muscle performance in old rats.</a> Physiol Rep. 12 (19), e70036(2024).</li>
<li>Hinks, A., Vlemmix, E., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Submaximal+eccentric+training+during+immobilization+does+not+prevent+serial+sarcomere+loss+or+impairments+in+mechanical+function+in+old+or+young+rats%5BTitle%5D)+AND+%22J+Gerontol+A+Biol+Sci+Med+Sci%22%5BJournal%5D)">Submaximal eccentric training during immobilization does not prevent serial sarcomere loss or impairments in mechanical function in old or young rats.</a> J Gerontol A Biol Sci Med Sci. 80 (7), glaf082(2025).</li>
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<li>Oh, S. S., Narver, H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+and+rat+anesthesia+and+analgesia%5BTitle%5D)+AND+%22Curr+Protoc%22%5BJournal%5D)">Mouse and rat anesthesia and analgesia.</a> Curr Protoc. 4 (2), e995(2024).</li>
<li>Paris, M. T., McNeil, C. J., Power, G. A., Rice, C. L., Dalton, B. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+performance+fatigability%3A+a+comprehensive+review+of+dynamic+tasks%5BTitle%5D)+AND+%22J+Appl+Physiol%22%5BJournal%5D)">Age-related performance fatigability: a comprehensive review of dynamic tasks.</a> J Appl Physiol. 133 (4), 850-866 (2022).</li>
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<li>Edman, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Double-hyperbolic+force-velocity+relation+in+frog+muscle+fibres%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">Double-hyperbolic force-velocity relation in frog muscle fibres.</a> J Physiol. 404 (1), 301-321 (1988).</li>
</ol>

<table><tbody><tr><td>1-1/2" chiodi con finitura liscia in acciaio zincato &nbsp;</td><td>The Hillman Group Canada ULC, Pickering, Ontario, Canada</td><td>https://shop.hillmangroup.ca/ccrz__ProductDetails?sku=461146&cclcl=en_US</td><td>Per il portaelettrodi su misura (istruzioni di costruzione fornite nella Figura Supplementare 1)</td></tr><tr><td>Apparecchiatura per ratti</td><td>Aurora Scientific Inc. (Aurora, Ontario, Canada)</td><td>806D&nbsp;</td><td></td></tr><tr><td>Accessorio personalizzato per fissare elettrodi progettato in Tinkercad (software open source) e stampato in 3D</td><td>Autodesk Tinkercad&nbsp;</td><td>www.tinkercad.com</td><td>Per il portaelettrodi su misura (istruzioni di costruzione fornite nella Figura Supplementare 1)</td></tr><tr><td>Stimolatore ad alta potenza bifase</td><td>Aurora Scientific Inc. (Aurora, Ontario, Canada)</td><td>701C</td><td></td></tr><tr><td>Parti LEGO</td><td>BrickLink.com</td><td>Vedi la Figura Supplementare S1 per i codici BrickLink.com di tutti i LEGO&reg; Parti</td><td>Per il portaelettrodi su misura (istruzioni di costruzione fornite nella Figura Supplementare 1)</td></tr><tr><td>Spectrum 360 Electrode Gel</td><td>Spectra</td><td>https://www.orthocanada.com/en/spectra-electrode-gel?utm_term=&utm_campaign=Canada+EN+-+Search+-+OrthoCanada&utm_source=adwords&utm_medium=ppc&hsa_acc=1971735470&hsa_cam=136169080&hsa_grp=139530010212&hsa_ad=586754644289&hsa_src=g&hsa_tgt=dsa-19959388920&hsa_kw=&hsa_mt=&hsa_net=adwords&hsa_ver=3&gad_source=1&gad_campaignid=136169080&gbraid=0AAAAAD1V26BmzV4eR9tkH_8Truu4W7uu6&gclid=Cj0KCQjwnJfEBhCzARIsAIMtfKLpFSpoPZdXDBtyR8sRgHYCfYuELZFPs4g8U6r8t4_chBuz5KF0pBIaAoQ3EALw_wcB</td><td></td></tr><tr><td>Unguento per gli occhi lubrificante notturno Systane</td><td>Systane</td><td>https://www.amazon.ca/dp/B004RSQGWC?ref=nb_sb_ss_w_as-reorder_k0_1_12&amp=&crid=38CVEZ82H7F4&amp=&sprefix=eye+ointment</td><td></td></tr><tr><td>Dieci femmine di ratti Sprague-Dawley</td><td>Charles River Laboratories (Senneville, QC, Canada)</td><td>Codice di deformazione: 001</td><td></td></tr><tr><td>Nastro chirurgico transpore</td><td>3M (Milton, Ontario, Canada)</td><td>1527</td><td></td></tr><tr><td>Crema Depilatoria Veet Pure</td><td>Veet</td><td>https://www.walmart.ca/en/ip/Veet-Pure-Hair-Removal-Cream-Legs-Body-Sensitive-Skin-400-mL/10032637</td><td></td></tr><tr><td>Scaldabagno/Circolatore</td><td>Aurora Scientific Inc. (Aurora, Ontario, Canada)</td><td>827A & nbsp;</td><td></td></tr></tbody></table>

costruzione di profili coppia-velocità-potenza a carico costante (isotonico) e a velocità costante (isocinetica) in vivo per i flessori plantari di ratto

Construction of Constant-Load (Isotonic) and Constant-Velocity (Isokinetic) Torque-Velocity-Power Profiles In vivo for the Rat Plantar Flexors

Costruzione di profili coppia-velocità-potenza a carico costante (isotonico) e a velocità costante (isocinetica) in vivo per i flessori plantari di ratto

Understanding the torque-velocity-power relationship of muscle is critical for assessing dynamic muscle performance, which has implications for health and ...

construction-constant-load-isotonic-constant-velocity-isokinetic

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JoVE Journal

Bioengineering

Bioingegneria

Descriviamo un metodo minimamente invasivo e indolore per misurare la forza muscolare degli arti posteriori del cane e la risposta muscolare alle ripetute contrazioni eccentriche.

In Vivo saggio canina funzione muscolare

Un In vivo Modello di roditore della contrazione indotta Lesioni e monitoraggio non invasivo di recupero

Questo video dimostra la preparazione chirurgica e le procedure necessarie per studiare le risposte contrattili del gastrocnemio mediale preparazione muscolare di...

Procedure di Rat<em&gt; In situ</em&gt; Proprietà muscolatura scheletrica contrattili

Presentiamo un protocollo per valutare i cambiamenti nella funzione neuromuscolare. La stimolazione nervosa elettrica percutanea è un metodo non invasivo che evoca risposte muscolari. Le proprietà elettrofisiologiche e meccaniche di queste risposte permettono di valutare la funzione neuromuscolare dal cervello al muscolo (a livello sopra-spinale, spinale e periferico).

Valutazione della Neuromuscolare Funzione Con percutanea stimolazione elettrica del nervo

Descriviamo un protocollo in vivo per misurare la dorsiflessione del piede in seguito alla stimolazione del nervo peroneo e alla contrazione del compartimento crurale anteriore dell'arto posteriore del ratto. Tali misurazioni sono uno strumento traslazionale indispensabile per valutare la patologia del muscolo scheletrico e gli approcci di ingegneria tissutale alla riparazione e alla rigenerazione muscolare.

applicazioni di<em&gt; In Vivo</em&gt; Test funzionale del Topo tibiale anteriore per la valutazione di ingegneria tessutale muscolo scheletrico di riparazione

La valutazione del recupero motorio rimane la misura di risultato di riferimento negli studi sperimentali sui nervi periferici. La misurazione della forza tetanica isometrica del muscolo tibiale anteriore nel ratto è uno strumento inestimabile per valutare gli esiti funzionali dopo la ricostruzione dei difetti nervosi sciatici. I metodi e le sfumature sono dettagliati in questo articolo.

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La quantificazione della forza massima dell'estensore del ginocchio è indispensabile per comprendere gli adattamenti funzionali all'invecchiamento, alla malattia, alle lesioni e alla riabilitazione. Presentiamo un nuovo metodo per misurare ripetutamente la coppia tetanica di picco isometrica dell'estensione del ginocchio in vivo.

Misurazione in vivo della funzione muscolare estensore del ginocchio nei topi

Il presente protocollo descrive dettagli sperimentali concisi sulla valutazione e l'interpretazione dei dati di coppia in vivo ottenuti tramite stimolazione elettrica del nervo peroneale comune in suini anestetizzati.

In vivo Misurazione della coppia isometrica del dorsiflexor hindlimb da pig

Questo articolo descrive in dettaglio la metodologia per emulare la produzione di forza muscolare in vivo durante esperimenti di ciclo di lavoro ex vivo utilizzando un muscolo "avatar" di un roditore di laboratorio per valutare i contributi dei transitori di deformazione e l'attivazione alla risposta della forza muscolare.

"Avatar", un ciclo di lavoro ex vivo modificato che utilizza la tensione e l'attivazione in vivo

Questo protocollo presenta un sistema di test utilizzato per indurre lesioni da fatica quantificabili e controllate in un tendine d'Achille di ratto per un modello in vivo di tendinopatia indotta da uso eccessivo. La procedura consiste nel fissare la caviglia del ratto a un attuatore articolare che esegue la flessione dorsale passiva della caviglia con uno script MATLAB scritto su misura.

Un sistema di test di flessione dorsale passiva della caviglia per un modello in vivo di tendinopatia indotta da uso eccessivo

Impara a costruire profili coppia-velocità-potenza in vivo nei flessori plantari di ratto usando stimolazione muscolare transcutanea. Questo metodo non invasivo permette di studiare la funzione muscolare e gli effetti delle malattie.

Costruzione di profili coppia-velocità-potenza a carico costante (isotonico) e a velocità costante (isocinetica) in vivo per i flessori plantari di ratto (Video) | JoVE Journal

Costruzione di profili coppia-velocità-potenza a carico costante (isotonico) e a velocità costante (isocinetica) in vivo per i flessori plantari di ratto

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