Name: Nuovo modello chirurgico di roditore per lo studio delle opzioni di trattamento del dolore del neuroma utilizzando la reinnervazione muscolare mirata attraverso il nervo safeno (Video) | JoVE Journal
Uploaded: 2025-11-14T13:55:00.000+00:00
Duration: 7 min
Description: Impara un modello chirurgico di ratto per studiare il dolore del neuroma e valutare trattamenti come la reinnervazione muscolare mirata. Questo metodo colma il divario tra ricerca preclinica e clinica.

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</ol>

<table><tbody><tr><td>Carprofen</td><td>Farmacia veterinaria locale</td><td>n/a</td><td>La farmacia veterinaria locale prepara la diluizione del carprofene</td></tr><tr><td>Cotton bob</td><td>Nobamed</td><td>974255</td><td>&mdash;</td></tr><tr><td>Rasoio elettrico</td><td>Esculap</td><td>GT421</td><td></td></tr><tr><td>Elettrocauterizzazione</td><td>Strumenti scientifici di qualità</td><td>18010-00</td><td>&mdash;</td></tr><tr><td>Etanolo 70%</td><td>Interchema BV</td><td>400406</td><td>&mdash;</td></tr><tr><td>Ethilon 10.0</td><td>Johnson & Johnson</td><td>BV130-3: codice 2820G</td><td></td></tr><tr><td>Ethilon 11.0</td><td>Johnson & Johnson</td><td>Codice BV50-3L 2881G</td><td></td></tr><tr><td>Ethilon 4.0</td><td>Johnson & Johnson</td><td>1854G: codice 1854G</td><td>-</td></tr><tr><td>Ethilon 6.0</td><td>Johnson & Johnson</td><td>BV130-5: codice 2809G</td><td></td></tr><tr><td>Ethilon 8.0</td><td>Johnson & Johnson</td><td>BV130-5: codice 2808G</td><td>&mdash;</td></tr><tr><td>Ethilon 9.0</td><td>Johnson & Johnson</td><td>BV130-3: codice 2819G</td><td></td></tr><tr><td>Guanti da esame nitrilo</td><td>Sempermed GmbH</td><td>1065920</td><td></td></tr><tr><td>Protezione oculare</td><td>3M & trade; Assistenza sanitaria</td><td>71512-00001M</td><td></td></tr><tr><td>Sterilizzatore a perline di vetro</td><td>Attrezzature da laboratorio VanPel</td><td>GBS300H</td><td></td></tr><tr><td>GraphPad Prism</td><td>GraphPad Software, San Diego, California USA</td><td>&nbsp; Versione 10</td><td></td></tr><tr><td>Cuscina riscaldante</td><td>Scienza della Condotta</td><td>CS-HP-2005</td><td></td></tr><tr><td>Isoflo (isoflurano)</td><td>Zoetis / Dechra Veterinary Products</td><td>B506</td><td>&mdash;</td></tr><tr><td>Lidocaina 1%</td><td>Henry Schein</td><td>809755</td><td></td></tr><tr><td>Gabbie a fondo a rete</td><td>Stoelting Co.</td><td>57816, 57824</td><td>&mdash;</td></tr><tr><td>Micro pinze</td><td>Strumenti scientifici di qualità</td><td>11251-35</td><td>&mdash;</td></tr><tr><td>Micro portaago</td><td>Strumenti scientifici di qualità</td><td>12076-12</td><td>&mdash;</td></tr><tr><td>Micro forbici</td><td>Strumenti scientifici di qualità</td><td>15019-10</td><td>&mdash;</td></tr><tr><td>Piccole pinzette</td><td>Strumenti scientifici di qualità</td><td>11254-20</td><td>&mdash;</td></tr><tr><td>NaCl 0,9%</td><td>Trademed</td><td>H7 1000-FRE</td><td>&mdash;</td></tr><tr><td>Ago 25G e volte; 1"</td><td>Becton, Dickinson & Azienda</td><td>300400</td><td></td></tr><tr><td>Portaaghi</td><td>Strumenti scientifici di qualità</td><td>12004-16</td><td>&mdash;</td></tr><tr><td>Stimolatore nervoso</td><td>&nbsp; (VARI-STIM Localizzatore di nervi)</td><td>REF 856200</td><td></td></tr><tr><td>Software RandoMice</td><td>van Eineige et al.&nbsp; Leiden</td><td>Versione 1.1.7</td><td></td></tr><tr><td>Sonda rettale per roditori</td><td>Strumenti di Precisione Mondiale</td><td>NC9506514</td><td></td></tr><tr><td>Bisturi</td><td>Strumenti scientifici di qualità</td><td>10003-12</td><td>&mdash;</td></tr><tr><td>Forbici</td><td>Strumenti scientifici di qualità</td><td>14001-12</td><td>&mdash;</td></tr><tr><td>Valutatore sensoriale</td><td>Strumentazione Linton</td><td>514007 e traccia; 514015</td><td>Monofilamenti da 0,6 g a 15 g</td></tr><tr><td>Microscopio chirurgico stereo</td><td>Leica</td><td>A60 F</td><td>&mdash;</td></tr><tr><td>Cuscinetti di garza sterili</td><td>Nobamed</td><td>740113</td><td></td></tr><tr><td>Unguento lubrificante oculare sterile</td><td>Alcon&nbsp;</td><td>&lrm; B00BRLSC8S</td><td></td></tr><tr><td>Foglio sterile con foro</td><td>Evercare / OneMed</td><td>1555-01</td><td>&mdash;</td></tr><tr><td>Lama chirurgica n. 15</td><td>Strumenti scientifici di qualità</td><td>10015-00</td><td>&mdash;</td></tr><tr><td>Maschera chirurgica</td><td>Medline Industries, Inc.</td><td>MSK1002</td><td></td></tr><tr><td>Siringa Luer Lock 2 ml</td><td>Becton, Dickinson & Azienda</td><td>300613</td><td></td></tr><tr><td>Pinzette</td><td>Strumenti scientifici di qualità</td><td>11617-12</td><td>&mdash;</td></tr></tbody></table>

nuovo modello chirurgico di roditore per lo studio delle opzioni di trattamento del dolore del neuroma utilizzando la reinnervazione muscolare mirata attraverso il nervo safeno

Novel Surgical Rodent Model for Studying Neuroma Pain Treatment Options using Targeted Muscle Reinnervation Through the Saphenous Nerve

Nuovo modello chirurgico di roditore per lo studio delle opzioni di trattamento del dolore del neuroma utilizzando la reinnervazione muscolare mirata attraverso il nervo safeno

This protocol describes a novel surgical model to study nerve pain and surgical treatment in rats. Traditional neuroma models use mixed nerves, although ...

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Il Risparmiata lesioni nervose modello animale è descritto qui come un modello murino di dolore neuropatico periferico dopo denervazione parziale del nervo sciatico con lesione dei rami del nervo tibiale e peroneale comune, lasciando i rimanenti nervo surale intatta. Modificazione comportamentale che derivi dalla meccanica allodinia è quantificato da von filamenti Frey.

Risparmiato il Nerve pregiudizio (SNI) Modello di Allodinia meccanica indotta nei topi

Fonte: Brakkee, E. M.,  et al. Chirurgia e test comportamentali nel modello di trasposizione del neuroma tibiale nei ratti. J. Vis. Exp. (2023). Questo video mostra una procedura per generare un modello di trasposizione del neuroma tibiale (TNT) di ratto. Dopo aver esposto il nervo tibiale nella zampa posteriore di un ratto anestetizzato, sezionare il nervo. Quindi, crea un tunnel sottocutaneo, infila il nervo reciso attraverso il tunnel e suturalo in posizione. In assenza di un bersaglio per...

Generazione di un modello di ratto di trasposizione del neuroma tibiale

Il dolore neuropatico è una conseguenza di una lesione o malattia che colpisce il sistema somatosensoriale. Il "modello bracciale" del dolore neuropatico nei topi consiste nella impiantazione di un bracciale polietilene attorno al ramo principale del nervo sciatico. Allodinia meccanica è testato con filamenti di von Frey.

Il nervo sciatico ammanettare modello di dolore neuropatico nei topi

La chirurgia modificata è un metodo semplificato per il modello di lesione nervosa risparmiata da topi o ratti che richiede solo una legatura e un taglio per ferire sia il nervo peroneo comune che quello surale.

Modello modificato di chirurgia delle lesioni nervose risparmiate del dolore neuropatico nei topi

La legatura parziale del nervo sciatico induce dolore neuropatico cronico di lunga durata, caratterizzato da risposte esagerate agli stimoli termici e meccanici. Questo modello murino di dolore neuropatico è comunemente usato per studiare terapie innovative per la gestione del dolore. Questo articolo descrive in dettaglio la procedura chirurgica per migliorare la standardizzazione e la riproducibilità.

Legatura parziale del nervo sciatico: un modello murino di dolore neuropatico cronico per studiare l'effetto antinocicettivo di nuove terapie

Questo protocollo descrive il modello di trasposizione del neuroma tibiale, che comporta una lesione del nervo tibiale con successiva trasposizione dell'estremità del nervo prossimale verso una posizione pretibiale o laterale sottocutanea. I test comportamentali del dolore neuromatico e dell'iperalgesia plantare sono quantificati utilizzando i monofilamenti di Von Frey.

Chirurgia e test comportamentali nel modello di trasposizione del neuroma tibiale nei ratti

Qui, descriviamo la procedura chirurgica per eseguire la chirurgia rigenerativa dell'interfaccia nervosa periferica (RPNI) per il trattamento del dolore neuropatico post-amputazione nel contesto di uno studio internazionale randomizzato controllato (RCT) (ClinicalTrials.gov, NCT05009394). L'RCT confronta l'RPNI con altre due tecniche chirurgiche, vale a dire la reinnervazione muscolare mirata (TMR) e l'escissione del neuroma combinata con la trasposizione intramuscolare.

Interfaccia rigenerativa del nervo periferico: protocollo chirurgico per uno studio controllato randomizzato nel dolore post-amputazione

Il protocollo delinea la procedura chirurgica per il trattamento del dolore post-amputazione mediante Reinnervazione Muscolare Mirata (TMR). La TMR sarà confrontata con altre due tecniche chirurgiche, in particolare l'interfaccia rigenerativa del nervo periferico (RPNI) e l'escissione del neuroma, seguita da un immediato seppellimento all'interno del muscolo nel contesto di uno studio internazionale randomizzato controllato.

Reinnervazione muscolare mirata: protocollo chirurgico per uno studio controllato randomizzato sul dolore post-amputazione

Questo protocollo dimostra come eseguire un'amputazione sotto il ginocchio nei ratti e come eseguire immediatamente una reinnervazione muscolare mirata (iTMR) per indagare strategie per trattare e prevenire il dolore correlato all'amputazione e per comprendere meglio i meccanismi dolorosi associati all'amputazione.

Modello di amputazione dell'arto posteriore del ratto sotto il ginocchio e reinnervazione muscolare mirata immediata

Utilizzo di metodi funzionali MRI e comportamentali per determinare la rappresentazione neurale del valore personale di opzioni rischiose e ambigue nel cervello umano.

Misurare il valore soggettivo di opzioni rischiose e ambigui utilizzando Economia Sperimentale e funzionali metodi MRI

Impara un modello chirurgico di ratto per studiare il dolore del neuroma e valutare trattamenti come la reinnervazione muscolare mirata. Questo metodo colma il divario tra ricerca preclinica e clinica.

Nuovo modello chirurgico di roditore per lo studio delle opzioni di trattamento del dolore del neuroma utilizzando la reinnervazione muscolare mirata attraverso il nervo safeno (Video) | JoVE Journal

Nuovo modello chirurgico di roditore per lo studio delle opzioni di trattamento del dolore del neuroma utilizzando la reinnervazione muscolare mirata attraverso il nervo safeno

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