Cardiac Blood Collection via Subclavian Puncture in Mice

Siyi Zhong; Qingya Dang; Yuting Ma; Shiyu Wang; Xin Bao; Hailian Quan; Lan Hong

doi:10.3791/69318

Raccolta di sangue cardiaca tramite puntura succlavia nei topi

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Cardiac Blood Collection via Subclavian Puncture in Mice

Raccolta di sangue cardiaca tramite puntura succlavia nei topi

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04:47 min

November 28, 2025

DOI: 10.3791/69318-v

Siyi Zhong*¹, Qingya Dang*², Yuting Ma², Shiyu Wang², Xin Bao², Hailian Quan², Lan Hong²

¹College of Agriculture,Yanbian University, ²Department of Physiology and Pathophysiology, School of Medicine,Yanbian University

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Overview

This study evaluates the efficacy of subclavian cardiac puncture as a method for collecting terminal blood in mice. Utilizing this technique improves sample purity and reliability compared to traditional blood collection methods, minimizing risks to the organism.

Key Study Components

Research Area

Cardiac blood collection
Mice as a model organism
Ethical considerations in animal research

Background

Blood sampling is crucial in scientific research involving mice.
Traditional methods have ethical limitations and risks.
The subclavian puncture method minimizes these concerns.

Methods Used

Subclavian puncture technique for blood collection
Mice (C57BL/6 strain) weighing between 21 and 29 grams
Use of heparin-coated microcentrifuge tubes for plasma transfer

Main Results

Blood volumes collected ranged from 700 to 1,200 microliters.
Sample purity was maintained, avoiding contamination.
No significant gender differences in results for ALT and AST levels.

Conclusions

Subclavian cardiac puncture is effective for high-purity blood collection in mice.
This method is vital for accurate experimental data and reduces animal stress responses.

Frequently Asked Questions

What are the advantages of subclavian cardiac puncture?

It offers a reliable method for high-purity blood sampling while minimizing risks of contamination and animal stress.

What volume of blood can be collected using this method?

Between 700 to 1,200 microliters in each collection.

How does this method compare to traditional techniques?

It avoids liver damage and related sample quality issues associated with abdominal puncture.

What precautions should be taken when performing the procedure?

Ensure proper anesthesia and syringe handling to avoid needle dislodgement.

Are there any measured biological effects from the blood samples?

ALT and AST levels were within normal ranges, indicating healthy physiological status.

Is subclavian puncture ethical?

Yes, it reduces stress on animals by minimizing the number of procedures required.

Can this method be applied to different mouse strains?

While this study used C57BL/6 mice, the technique can be adapted for other strains as well.

La puntura cardiaca della sottoclavia è un metodo semplice e affidabile per raccogliere sangue terminale nei topi. È facile da usare e garantisce campioni ad alta purezza.

Raccolta di sangue cardiaca tramite puntura succlavia nei topi. Il prelievo di sangue nei topi svolge un ruolo significativo nella ricerca scientifica. La puntura cardiaca e la raccolta di sangue su grandi vasi possono ottenere grandi volumi sanguigni nelle procedure terminali.

Tuttavia, la puntura cardiaca ha ricevuto una crescente attenzione a causa di limitazioni etiche e di rischi associati più elevati. In questo studio, abbiamo utilizzato il metodo della puntura della succlavia per il prelievo di sangue cardiaco. Rispetto all'approccio tradizionale della puntura addominale, questo metodo evita efficacemente danni al fegato, migliorando così la qualità del campione e l'affidabilità dei dati sperimentali.

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