Name: Determinazione della biomassa microbica nel suolo tramite Digital Droplet PCR (ddPCR) (Video) | JoVE Journal
Uploaded: 2026-04-24T14:20:00.000+00:00
Duration: 7 min 46 s
Description: Scopri come quantificare la biomassa microbica nel suolo con ddPCR, un metodo sensibile che richiede campioni minimi e supera i limiti delle tecniche convenzionali.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Jobbágy, E. G., Jackson, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+vertical+distribution+of+soil+organic+carbon+and+its+relation+to+climate+and+vegetation%5BTitle%5D)+AND+%22Ecol+Appl%22%5BJournal%5D)">The vertical distribution of soil organic carbon and its relation to climate and vegetation.</a> Ecol Appl. 10 (2), 423-436 (2000).</li>
<li>Sanderman, J., Hengl, T., Fiske, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Soil+carbon+debt+of+12%2C000+years+of+human+land+use%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Soil carbon debt of 12,000 years of human land use.</a> Proc Natl Acad Sci U S A. 114 (36), 9575-9580 (2017).</li>
<li>He, Y., Li, J., Siemann, E., Li, B., Xu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Plant+invasion+increases+soil+microbial+biomass+carbon%3A+meta-analysis+and+empirical+tests%5BTitle%5D)+AND+%22Glob+Change+Biol%22%5BJournal%5D)">Plant invasion increases soil microbial biomass carbon: meta-analysis and empirical tests.</a> Glob Change Biol. 31 (3), e70109(2025).</li>
<li>de Menezes, A. B., Richardson, A. E., Thrall, P. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Linking+fungal-bacterial+co-occurrences+to+soil+ecosystem+function%5BTitle%5D)+AND+%22Curr+Opin+Microbiol%22%5BJournal%5D)">Linking fungal-bacterial co-occurrences to soil ecosystem function.</a> Curr Opin Microbiol. 37, 135-141 (2017).</li>
<li>Wang, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((ddPCR+surpasses+classical+qPCR+technology+in+quantitating+bacteria+and+fungi+in+the+environment%5BTitle%5D)+AND+%22Mol+Ecol+Resour%22%5BJournal%5D)">ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment.</a> Mol Ecol Resour. 22 (7), 2587-2598 (2022).</li>
<li>Djemiel, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biogeographical+patterns+of+the+soil+fungal%3Abacterial+ratio+across+France%5BTitle%5D)+AND+%22mSphere%22%5BJournal%5D)">Biogeographical patterns of the soil fungal:bacterial ratio across France.</a> mSphere. 8 (5), e0036523(2023).</li>
<li>Bailey, V. L., Smith, J. L., Bolton, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fungal-to-bacterial+ratios+in+soils+investigated+for+enhanced+C+sequestration%5BTitle%5D)+AND+%22Soil+Biol+Biochem%22%5BJournal%5D)">Fungal-to-bacterial ratios in soils investigated for enhanced C sequestration.</a> Soil Biol Biochem. 34 (7), 997-1007 (2002).</li>
<li>Vance, E. D., Brookes, P. C., Jenkinson, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbial+biomass+measurements+in+forest+soils%3A+the+use+of+the+chloroform+fumigation-incubation+method+in+strongly+acid+soils%5BTitle%5D)+AND+%22Soil+Biol+Biochem%22%5BJournal%5D)">Microbial biomass measurements in forest soils: the use of the chloroform fumigation-incubation method in strongly acid soils.</a> Soil Biol Biochem. 19 (6), 697-702 (1987).</li>
<li>Frostegård, Å, Bååth, E., Tunlio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Shifts+in+the+structure+of+soil+microbial+communities+in+limed+forests+as+revealed+by+phospholipid+fatty+acid+analysis%5BTitle%5D)+AND+%22Soil+Biol+Biochem%22%5BJournal%5D)">Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis.</a> Soil Biol Biochem. 25 (6), 723-730 (1993).</li>
<li>Cao, Z., Li, D., Han, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+fungal+to+bacterial+ratio+in+soil+food+webs%2C+and+its+measurement%5BTitle%5D)+AND+%22Acta+Ecol+Sin%22%5BJournal%5D)">The fungal to bacterial ratio in soil food webs, and its measurement.</a> Acta Ecol Sin. 31 (20), 6050-6058 (2011).</li>
<li>Frossard, A., Hammes, F., Gessner, M. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Flow+cytometric+assessment+of+bacterial+abundance+in+soils%2C+sediments+and+sludge%5BTitle%5D)+AND+%22Front+Microbiol%22%5BJournal%5D)">Flow cytometric assessment of bacterial abundance in soils, sediments and sludge.</a> Front Microbiol. 7, 903(2016).</li>
<li>Joergensen, R. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+hitchhiker%E2%80%99s+guide%3A+Estimates+of+microbial+biomass+and+microbial+gene+abundance+in+soil%5BTitle%5D)+AND+%22Biol+Fertil+Soils%22%5BJournal%5D)">A hitchhiker’s guide: Estimates of microbial biomass and microbial gene abundance in soil.</a> Biol Fertil Soils. 60 (4), 457-470 (2024).</li>
<li>Fierer, N., Wood, S. A., Bueno de Mesquita, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((How+microbes+can%2C+and+cannot%2C+be+used+to+assess+soil+health%5BTitle%5D)+AND+%22Soil+Biol+Biochem%22%5BJournal%5D)">How microbes can, and cannot, be used to assess soil health.</a> Soil Biol Biochem. 153, 108111(2021).</li>
<li>Laine, M. B., Taipale, S. J., Tiirola, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+methods+for+assessing+fungi-to-bacteria+ratio+of+soil%5BTitle%5D)+AND+%22Biol+Fertil+Soils%22%5BJournal%5D)">Comparison of methods for assessing fungi-to-bacteria ratio of soil.</a> Biol Fertil Soils. 61 (5), 941-954 (2025).</li>
<li>De Filippis, F., Laiola, M., Blaiotta, G., Ercolini, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Different+amplicon+targets+for+sequencing-based+studies+of+fungal+diversity%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Different amplicon targets for sequencing-based studies of fungal diversity.</a> Appl Environ Microbiol. 83 (17), e00905-e00917 (2017).</li>
<li>Liu, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((FungiQuant%3A+A+broad-coverage+fungal+quantitative+real-time+PCR+assay%5BTitle%5D)+AND+%22BMC+Microbiol%22%5BJournal%5D)">FungiQuant: A broad-coverage fungal quantitative real-time PCR assay.</a> BMC Microbiol. 12, 255(2012).</li>
<li>Lofgren, L. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Genome-based+estimates+of+fungal+rDNA+copy+number+variation+across+phylogenetic+scales+and+ecological+lifestyles%5BTitle%5D)+AND+%22Mol+Ecol%22%5BJournal%5D)">Genome-based estimates of fungal rDNA copy number variation across phylogenetic scales and ecological lifestyles.</a> Mol Ecol. 28 (4), 721-730 (2019).</li>
<li>Angly, F. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((W.+CopyRighter%3A+A+rapid+tool+for+improving+the+accuracy+of+microbial+community+profiles+through+lineage-specific+gene+copy+number+correction%5BTitle%5D)+AND+%22Microbiome%22%5BJournal%5D)">W. CopyRighter: A rapid tool for improving the accuracy of microbial community profiles through lineage-specific gene copy number correction.</a> Microbiome. 2 (1), 11(2014).</li>
<li>Kembel, S. W., Wu, M., Eisen, J. A., Green, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Incorporating+16S+gene+copy+number+information+improves+estimates+of+microbial+diversity+and+abundance%5BTitle%5D)+AND+%22PLoS+Comput+Biol%22%5BJournal%5D)">Incorporating 16S gene copy number information improves estimates of microbial diversity and abundance.</a> PLoS Comput Biol. 8 (10), e1002743(2012).</li>
<li>Pan, P., Gu, Y., Sun, D. L., Wu, Q. L., Zhou, N. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbial+diversity+biased+estimation+caused+by+intragenomic+heterogeneity+and+interspecific+conservation+of+16S+rRNA+genes%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Microbial diversity biased estimation caused by intragenomic heterogeneity and interspecific conservation of 16S rRNA genes.</a> Appl Environ Microbiol. 89 (5), e0210822(2023).</li>
<li>Chacón-Vargas, K., Torres, J., Giles-Gómez, M., Escalante, A., Gibbons, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Genomic+profiling+of+bacterial+and+fungal+communities+and+their+predictive+functionality+during+pulque+fermentation+by+whole-genome+shotgun+sequencing%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Genomic profiling of bacterial and fungal communities and their predictive functionality during pulque fermentation by whole-genome shotgun sequencing.</a> Sci Rep. 10 (1), 15115(2020).</li>
<li>Bahram, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metagenomic+assessment+of+the+global+diversity+and+distribution+of+bacteria+and+fungi%5BTitle%5D)+AND+%22Environ+Microbiol%22%5BJournal%5D)">Metagenomic assessment of the global diversity and distribution of bacteria and fungi.</a> Environ Microbiol. 23 (1), 316-326 (2021).</li>
<li>Smith, C. J., Osborn, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advantages+and+limitations+of+quantitative+PCR+%28Q-PCR%29-based+approaches+in+microbial+ecology%3A+Application+of+Q-PCR+in+microbial+ecology%5BTitle%5D)+AND+%22FEMS+Microbiol+Ecol%22%5BJournal%5D)">Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology: Application of Q-PCR in microbial ecology.</a> FEMS Microbiol Ecol. 67 (1), 6-20 (2009).</li>
<li>Hindson, B. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-throughput+droplet+digital+PCR+system+for+absolute+quantitation+of+DNA+copy+number%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">High-throughput droplet digital PCR system for absolute quantitation of DNA copy number.</a> Anal Chem. 83 (22), 8604-8610 (2011).</li>
<li>Le Geay, M., Mayers, K., Küttim, M., Lauga, B., Jassey, V. E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+digital+droplet+PCR+approach+for+the+quantification+of+soil+micro-organisms+involved+in+atmospheric+CO2+fixation%5BTitle%5D)+AND+%22Environ+Microbiol%22%5BJournal%5D)">Development of a digital droplet PCR approach for the quantification of soil micro-organisms involved in atmospheric CO2 fixation.</a> Environ Microbiol. 26 (6), e16666(2024).</li>
<li>Hansen, P. M., Semenova-Nelsen, T. A., Platt, W. J., Sikes, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recurrent+fires+do+not+affect+the+abundance+of+soil+fungi+in+a+frequently+burned+pine+savanna%5BTitle%5D)+AND+%22Fungal+Ecol%22%5BJournal%5D)">Recurrent fires do not affect the abundance of soil fungi in a frequently burned pine savanna.</a> Fungal Ecol. 42, 100852(2019).</li>
<li>Ovreås, L., Forney, L., Daae, F. L., Torsvik, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Distribution+of+bacterioplankton+in+meromictic+Lake+Saelenvannet%2C+as+determined+by+denaturing+gradient+gel+electrophoresis+of+PCR-amplified+gene+fragments+coding+for+16S+rRNA%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.</a> Appl Environ Microbiol. 63 (9), 3367-3373 (1997).</li>
<li>Whitman, W. B., Coleman, D. C., Wiebe, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prokaryotes%3A+The+unseen+majority%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Prokaryotes: The unseen majority.</a> Proc Natl Acad Sci U S A. 95 (12), 6578-6583 (1998).</li>
<li>Cheng, J. Y. W., Chan, C. K., Lee, C. T., Lau, A. P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Carbon+content+of+common+airborne+fungal+species+and+fungal+contribution+to+aerosol+organic+carbon+in+a+subtropical+city%5BTitle%5D)+AND+%22Atmos+Environ%22%5BJournal%5D)">Carbon content of common airborne fungal species and fungal contribution to aerosol organic carbon in a subtropical city.</a> Atmos Environ. 43 (17), 2781-2787 (2009).</li>
<li>Wood, D. E., Lu, J., Langmead, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improved+metagenomic+analysis+with+Kraken+2%5BTitle%5D)+AND+%22Genome+Biol%22%5BJournal%5D)">Improved metagenomic analysis with Kraken 2.</a> Genome Biol. 20 (1), 257(2019).</li>
<li>Rousk, J., Brookes, P. C., Bååth, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Contrasting+soil+pH+effects+on+fungal+and+bacterial+growth+suggest+functional+redundancy+in+carbon+mineralization%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization.</a> Appl Environ Microbiol. 75 (6), 1589-1596 (2009).</li>
<li>Xie, Z., Canalda-Baltrons, A., d’Enfert, C., Manichanh, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Shotgun+metagenomics+reveals+interkingdom+association+between+intestinal+bacteria+and+fungi+involving+competition+for+nutrients%5BTitle%5D)+AND+%22Microbiome%22%5BJournal%5D)">Shotgun metagenomics reveals interkingdom association between intestinal bacteria and fungi involving competition for nutrients.</a> Microbiome. 11 (1), 275(2023).</li>
<li>Větrovský, T., Baldrian, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+variability+of+the+16S+rRNA+gene+in+bacterial+genomes+and+its+consequences+for+bacterial+community+analyses%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">The variability of the 16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses.</a> PLoS One. 8 (2), e57923(2013).</li>
<li>Doyle, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Absolute+quantification+of+prokaryotes+in+the+microbiome+by+16S+rRNA+qPCR+or+ddPCR%5BTitle%5D)+AND+%22Nat+Protoc%22%5BJournal%5D)">Absolute quantification of prokaryotes in the microbiome by 16S rRNA qPCR or ddPCR.</a> Nat Protoc. 20, 3471-3476 (2025).</li>
<li>Frostegård, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantification+of+bias+related+to+the+extraction+of+DNA+directly+from+soils%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Quantification of bias related to the extraction of DNA directly from soils.</a> Appl Environ Microbiol. 65 (12), 5409-5420 (1999).</li>
<li>Lee, E., Lim, H. J., Son, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Discrepancies+in+qPCR-based+gene+quantification+and+their+dependencies+on+soil+properties%2C+inhibitor+presence%2C+and+DNA+extraction+kit+types%5BTitle%5D)+AND+%22RSC+Adv%22%5BJournal%5D)">Discrepancies in qPCR-based gene quantification and their dependencies on soil properties, inhibitor presence, and DNA extraction kit types.</a> RSC Adv. 15 (25), 19656-19664 (2025).</li>
<li>Buell, Z. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Interrelationships+among+methods+of+estimating+microbial+biomass+across+multiple+soil+orders+and+biomes%5BTitle%5D)+AND+%22Soil+Biol+Biochem%22%5BJournal%5D)">Interrelationships among methods of estimating microbial biomass across multiple soil orders and biomes.</a> Soil Biol Biochem. 208, 109844(2025).</li>
<li>Carini, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Relic+DNA+is+abundant+in+soil+and+obscures+estimates+of+soil+microbial+diversity%5BTitle%5D)+AND+%22Nat+Microbiol%22%5BJournal%5D)">Relic DNA is abundant in soil and obscures estimates of soil microbial diversity.</a> Nat Microbiol. 2 (3), 16242(2016).</li>
<li>Child, H. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Automated+environmental+metagenomics+using+Oxford+nanopore+sequencing%5BTitle%5D)+AND+%22BMC+Genomics%22%5BJournal%5D)">Automated environmental metagenomics using Oxford nanopore sequencing.</a> BMC Genomics. 26 (1), 835(2025).</li>
<li>Paliaga, S., Laudicina, V. A., Muscarella, S. M., Said-Pullicino, D., Badalucco, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+different+methods+for+estimating+microbial+biomass+in+biochar-amended+soils%5BTitle%5D)+AND+%22Soil+Biol+Biochem%22%5BJournal%5D)">Comparison of different methods for estimating microbial biomass in biochar-amended soils.</a> Soil Biol Biochem. 203, 109733(2025).</li>
<li>Setia, R., Verma, S. L., Marschner, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Measuring+microbial+biomass+carbon+by+direct+extraction%3A+comparison+with+chloroform+fumigation-extraction%5BTitle%5D)+AND+%22Eur+J+Soil+Biol%22%5BJournal%5D)">Measuring microbial biomass carbon by direct extraction: comparison with chloroform fumigation-extraction.</a> Eur J Soil Biol. 53, 103-106 (2012).</li>
</ol>

<table><tbody><tr><td>Bettersizer S3 Plus&nbsp;</td><td>Bettersize, Cina</td><td>BT-803</td><td></td></tr><tr><td>Centrifuga</td><td>Termoscientifica&nbsp;</td><td>SL16&nbsp;</td><td></td></tr><tr><td>Cloroformio&nbsp;</td><td>ThermoScientific</td><td>L14759. AU & nbsp;</td><td></td></tr><tr><td>ddPCR Piastre a 96 pozzi</td><td>Bio-Rad, Watford, Regno Unito</td><td>12001925</td><td></td></tr><tr><td>ddPCR Olio per Lettore di Gocce &nbsp;</td><td>Bio-Rad, Watford, Regno Unito</td><td>1863004</td><td></td></tr><tr><td>Essiccatore&nbsp;</td><td>Davisil</td><td></td><td></td></tr><tr><td>Portacartucce DG8</td><td>Bio-Rad, Watford, Regno Unito</td><td>1863051</td><td></td></tr><tr><td>Cartucce DG8 per generatore di gocce QX200/QX100</td><td>Bio-Rad, Watford, Regno Unito</td><td>1864008</td><td></td></tr><tr><td>Guarnizioni DG8 per generatore di gocce QX200/QX100</td><td>Bio-Rad, Watford, Regno Unito</td><td>Q33265</td><td></td></tr><tr><td>DNeasy Power Soil pro kit & nbsp;</td><td>Qiagen, Germania</td><td>47017</td><td></td></tr><tr><td>Kit di analisi a doppio filamento (dsDNA) Broad Range (BR)</td><td>Invitrogen, Germania</td><td>Q33230</td><td></td></tr><tr><td>FastPrep-24 5G&nbsp;</td><td>MP Biomedicals, Regno Unito</td><td>116005500</td><td></td></tr><tr><td>GridION&nbsp;</td><td>Oxford Nanopore Technologies, Regno Unito</td><td>GRD-MK1CAPX</td><td></td></tr><tr><td>K2SO4</td><td>Merck, Regno Unito</td><td>7778-80-5</td><td></td></tr><tr><td>Perle magnetiche Mag-Bind</td><td>Omega Bio-tek, USA</td><td>M1378-01</td><td></td></tr><tr><td>Cella di flusso MinION</td><td>Oxford Nanopore Technologies, Regno Unito</td><td>FLO-MIN114</td><td></td></tr><tr><td>Caldaia ovattata</td><td>Carbolite&nbsp;</td><td>AAF1100</td><td></td></tr><tr><td>Kit nativo di codice a barre 24</td><td>Oxford Nanopore Technologies, Regno Unito</td><td>SQK-NBD114.24</td><td></td></tr><tr><td>Sigillatura termica per piastre PCR, stagnola, perforabili &nbsp;</td><td>Bio-Rad, Watford, Regno Unito</td><td>1814040</td><td></td></tr><tr><td>Sigillante per piastre PCR PX1</td><td>Bio-Rad, Watford, Regno Unito</td><td>1814000</td><td></td></tr><tr><td>Fluorometro a Qubit</td><td>Invitrogen, Germania</td><td>D33226</td><td></td></tr><tr><td>Sistema PCR digitale a gocce QX200</td><td>Bio-Rad, Watford, Regno Unito</td><td>1864001</td><td></td></tr><tr><td>QX200 ddPCR EvaGreen Supermix</td><td>Bio-Rad, Watford, Regno Unito</td><td>186-4033</td><td></td></tr><tr><td>QX200 Olio per la generazione di gocce per EvaGreen & nbsp;</td><td>Bio-Rad, Watford, Regno Unito</td><td>1864005</td><td></td></tr><tr><td>QXDx Generatore di Gocce&nbsp;</td><td>Bio-Rad, Watford, Regno Unito</td><td>12001049</td><td></td></tr><tr><td>SENSOQUEST LABCYCLER&nbsp;</td><td>Geneflow, Germania</td><td>1120280125</td><td></td></tr></tbody></table>

determinazione della biomassa microbica nel suolo tramite digital droplet pcr (ddpcr)

Determination of Microbial Biomass in Soil using Digital Droplet PCR (ddPCR)

Determinazione della biomassa microbica nel suolo tramite Digital Droplet PCR (ddPCR)

Soil represents the largest terrestrial pool of organic carbon, yet more than 133 Gt of carbon has been lost from soils over the past two centuries. ...

determination-microbial-biomass-soil-using-digital-droplet-pcr

Research

Ricerca

JoVE Journal

Environment

Ambiente

Descriviamo un saggio digitale endpoint per quantificare gli acidi nucleici con una lettura semplificata (analogica). Misuriamo la fluorescenza di massa di saggi digitali basati su goccioline utilizzando una macchina qPCR standard piuttosto che una strumentazione specializzata e confermiamo i nostri risultati mediante microscopia.

Semplice Lettura di massa di Digital acido nucleico quantificazione Assays

Questo manoscritto descrive un saggio PCR duplex digitale che può essere utilizzato per quantificare contemporaneamente Enterococcus spp. E marcatori genetici HF183 come indicatori di contaminazione fecale generale e umana associati nelle acque ricreative.

Un Test PCR duplex digitale per simultanea quantificazione del<em&gt; Enterococcus spp.</em&gt; E l&#39;umano fecale associata HF183 Marker in Waters

Viene descritto un metodo sensibile e accurato per la quantificazione di microRNA privi di cellule utilizzando una chimica basata su coloranti e una tecnologia PCR digitale a goccioline.

Di circolazione MicroRNA Quantificazione Utilizzando DNA-binding Dye Chimica e di gocce Digital PCR

Rilevamento rapido e preciso di agenti patogeni in loco, soprattutto terricoli fitopatogeni, è fondamentale per impedire ulteriore produzione di inoculo e proliferazione di malattie delle piante in campo. Il metodo sviluppato qui utilizzando un sistema di rilevazione portatile in tempo reale di PCR consente la diagnosi in loco in condizioni di campo.

Rilevamento in loco molecolare di fitopatogeni terricoli, utilizzando un sistema PCR in tempo reale portatile

Qui, descriviamo un protocollo per ottenere i dati di sequenza di amplicon del suolo, rizosfera e radice endosphere microbiomi. Questa informazione può essere utilizzata per studiare la composizione e la diversità delle comunità microbiche pianta-collegato ed è adatta per l'uso con una vasta gamma di specie di piante.

Esplorare il microbioma radice: estrazione dei dati di comunità batteriche dal suolo e la rizosfera radice Endosphere

Di seguito è descritta una procedura operativa standard semplificata per la profilazione del microbioma che utilizza il sequenziamento e l'analisi metagenomica del rRNA 16S utilizzando strumenti liberamente disponibili. Questo protocollo aiuterà i ricercatori che non hanno familiarità con il campo del microbioma e quelli che richiedono aggiornamenti sui metodi per ottenere una profilazione batterica a una risoluzione più elevata.

Analisi microbiota utilizzando PCR in due fasi e sequenziamento genico rRNA 16S di nuova generazione

Qui presentiamo un protocollo per misurare il numero assoluto di copie del DNA mitocondriale (mt) e i livelli di eteroplasmia di delezione del mtDNA in singole cellule.

Misurazione del numero di copie del DNA mitocondriale a singola cellula e dell'eteroplasmia utilizzando la reazione a catena della polimerasi digitale a goccia

Qui, presentiamo un protocollo per lo sviluppo e la convalida di buone pratiche di laboratorio nella rilevazione conforme di vettori virali adeno-associati in lacrime umane mediante reazione a catena della polimerasi digitale a supporto dello sviluppo clinico di vettori di terapia genica.

Un saggio di reazione a catena della polimerasi digitale a goccia validabile per la rilevazione di vettori virali adeno-associati negli studi di bioshedding delle lacrime

La quantificazione precisa delle copie del genoma del vettore del virus adeno-associato (AAV) è fondamentale, ma un protocollo standardizzato deve ancora essere stabilito. Questo protocollo descrive un metodo convalidato per la preparazione di campioni di AAV purificati e per l'esecuzione di una reazione a catena della polimerasi (dd_PCR) digitale per quantificare in modo affidabile il titolo del genoma virale.

Quantificazione dei genomi virali adeno-associati in campioni di vettori purificati mediante reazione a catena della polimerasi a goccioline digitali

Forme affidabili e accurate di analisi del DNA dispiegabile sul campo sono fondamentali per molti settori. Questo articolo offre due metodi per l'analisi microbica ambientale sul campo: 1) l'isolamento del DNA microbico da campioni ambientali utilizzando un kit di estrazione biologica sul campo, e 2) l'analisi del DNA tramite l'unità qPCR di campo.

Analisi del DNA microbico sul campo utilizzando un kit di estrazione biologica sul campo e un'unità qPCR sul campo

Scopri come quantificare la biomassa microbica nel suolo con ddPCR, un metodo sensibile che richiede campioni minimi e supera i limiti delle tecniche convenzionali.

Determinazione della biomassa microbica nel suolo tramite Digital Droplet PCR (ddPCR)

Esplora altri video