Name: Imaging a due fotoni intravitali della morfologia degli assoni sensoriali del tatto nella pelle del topo (Video) | JoVE Journal
Uploaded: 2025-12-30T13:50:00.000+00:00
Duration: 7 min 51 s
Description: Scopri l'imaging a due fotoni intravitale per visualizzare i terminali assonali LTMR nella pelle della zampa anteriore del topo. Questo metodo consente studi ad alta risoluzione sullo sviluppo dei circuiti somatosensoriali in vivo.

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</ol>

<table><tbody><tr><td>Obiettivo 20x & ndash; Super NA 1.0</td><td>Olympus</td><td>1-U2B965</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Copertura 22x60</td><td>VWR</td><td>16004-330</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Argilla da modellare con indurimento all'aria</td><td>DAS</td><td></td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Pompa d'aria per anestesia (HF), 110V</td><td>RWD Life Science Inc.</td><td>R510-30</td><td>Sistema isoflurano</td></tr><tr><td>Maschera Concentrica per Anestesia</td><td>RWD Life Science Inc.</td><td>R510-M</td><td>Sistema isoflurano</td></tr><tr><td>Camera di induzione per anestesia &ndash; Topo e Topo</td><td>RWD Life Science Inc.</td><td>V105</td><td>Sistema isoflurano</td></tr><tr><td>Bruker Ultima Investigator Plus Microscopio a Scansione Laser</td><td>Bruker Nano, Inc.&nbsp;</td><td></td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Aghi Farfalla</td><td>Fisher Scientific</td><td>1484041</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Chameleon Discovery NX con TPC: Dual</td><td>Coerente</td><td>1385777</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Maschera a cono con tubo per topi neonatali</td><td>RWD Life Science Inc.</td><td>68680</td><td>Sistema isoflurano</td></tr><tr><td>Doppi filtri a emissione (et525/70m e et595/50m) e t565lpxr&nbsp; Divisore di fascio dicroico</td><td>Nikon</td><td>INVP-01</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Set di filtri a epifluorescenza ET-GFP ET470/40x e ET252/50m; Dicroico T495LPXR&nbsp;</td><td>Nikon</td><td>518065</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Etanolo</td><td>Decon Laboratories Inc.</td><td></td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Piscino riscaldante a infrarossi lontani con controller (alimentazione AC)</td><td>Kent Scientific</td><td>RT-0515</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Rilevatori GaAsp (2)&nbsp;</td><td>Hamamatsu Photonics&nbsp;</td><td>HED-UP-2&nbsp;</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Contenitori del filtro a gas</td><td>RWD Life Science Inc.</td><td>R510-31-6</td><td>Sistema isoflurano</td></tr><tr><td>Grasso ad alto vuoto</td><td>Sigma Aldrich</td><td>Z273554</td><td>Sistema di imaging</td></tr><tr><td>Isoflurano, USP (250 mL)</td><td>Piramal Critical Care</td><td>6679-017-25</td><td>Sistema isoflurano</td></tr><tr><td>Scatola per copertura laser & nbsp;</td><td>Teco Technology</td><td></td><td>Sistema di imaging</td></tr><tr><td>Topi: B6.129S6(Cg)-Ntrk2tm3.1(cre/ERT2)Ddg/J</td><td>JAX</td><td>27214</td><td></td></tr><tr><td>Topoli: B6. Cg-Igs7tm140.1(tetO-EGFP, CAG-tTA2)Hze/J</td><td>JAX</td><td>30220</td><td></td></tr><tr><td>Topoli: B6. Cg-Tg(Thy1-YFP)16Jrs/J</td><td>Fiume Charles</td><td>3709</td><td></td></tr><tr><td>Crema Depilatoria Nair</td><td>Amazon</td><td>28001</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>File di dati grezzi</td><td>Zenodo</td><td></td><td>10.5281/zenodo.17370834</td></tr><tr><td>Supporto rigido con grande portainserti rettangolare</td><td>Thorlabs</td><td>MP15M</td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Piattaforma Operativa per Anestesia per Piccoli Animali</td><td>RWD Life Science Inc.</td><td>R510-PGL</td><td>Sistema isoflurano</td></tr><tr><td>Unguento Lubrificante per gli Occhi</td><td>Bausch + Lomb</td><td></td><td>Configurazione del microscopio a due fotoni</td></tr><tr><td>Vetri Superfrost Plus per microscopio</td><td>Fisher Scientific</td><td>12-550-15</td><td>Configurazione del microscopio a due fotoni</td></tr></tbody></table>

imaging a due fotoni intravitali della morfologia degli assoni sensoriali del tatto nella pelle del topo

Intravital Two-Photon Imaging of Touch Sensory Axon Morphology in Mouse Skin

In questo lavoro, la microscopia a due fotoni è stata utilizzata per visualizzare le strutture assonali Aβ RA-LTMR e Aδ-LTMR nella pelle durante lo sviluppo e negli adulti. Le terminazioni lanceolate mature, che si verificano nei topi intorno al giorno 20 e superiore al postnatale, sono completamentesviluppate 19,32. L'organizzazione complessiva dei terminali assonici nella pelle pelosa dei topi adulti è stata fotografata, come mostrato nella proiezione di massim...

Imaging a due fotoni intravitali della morfologia degli assoni sensoriali del tatto nella pelle del topo

Low-threshold mechanoreceptors (LTMRs) are somatosensory neurons that detect innocuous light touch stimuli such as vibration, hair deflection, and ...

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Neuroscienze

Imaging in diretta degli assoni spinali dopo rizotomia

Source: Mizuno, H., et al. In Vivo Imaging a due fotoni dei neuroni corticali nei topi neonatali. J. Vis. Exp. (2018)Questo video mostra la microscopia a due fotoni utilizzata per visualizzare i neuroni nel cervello di un cucciolo di topo transgenico anestetizzato. Il laser a due fotoni consente immagini più approfondite con un'elevata risoluzione spaziale e una dispersione della luce minima, consentendo l'acquisizione dettagliata della morfologia neuronale, comprese le proiezioni dendritiche.

Microscopia a due fotoni per l'imaging della morfologia neuronale in un cucciolo di topo

Per studiare i cambiamenti di fibre nervose nocicettive intraepidermiche (IENFs) nelle neuropatie dolorose (PN), abbiamo sviluppato protocolli che potrebbe esaminare direttamente le variazioni morfologiche tridimensionali osservati in IENFs nocicettivi. Analisi tridimensionale IENFs ha il potenziale di valutare le variazioni morfologiche del IENF in PN.

Imaging tridimensionale di fibre nervose nocicettive intraepidermiche in biopsie cutanee umane

Il protocollo per l'imaging longitudinale dinamico e selettivo lesione laser di terminazioni nervose in topi transgenici giornalista è presentato.

In Vivo microscopia a due fotoni di Endings singolo nervo in pelle

L'imaging time-lapse nell'animale vivente fornisce informazioni preziose sulla riorganizzazione strutturale nel cervello intatto. Qui, introduciamo una preparazione di cranio assottigliato che consente l'imaging transcranico di strutture sinaptiche marcate in fluorescenza nella corteccia di topo vivente mediante microscopia a due fotoni.

Two-Photon<em&gt; In vivo</em&gt; Imaging di spine dendritiche nella corteccia mouse Uso un teschio Diluito-Preparazione

Pelle dei mammiferi contiene una gamma diversificata di strutture - come i follicoli piliferi e le terminazioni nervose - che presentano modelli distintivi di organizzazione spaziale. Analizzando pelle come cavalcatura piatto approfitta della geometria 2-dimensionale di questo tessuto per produrre tutto spessore immagini ad alta risoluzione delle strutture cutanee.

Appartamento Monte Imaging di pelle di topo e la sua applicazione alla analisi del follicolo pilifero patterning e sensoriale Axon Morfologia

L'imaging intravitale a due fotoni può essere utilizzato per studiare le interazioni tra diversi tipi di cellule nel midollo spinale nel loro ambiente tissutale nativo in un animale chimerico del midollo osseo con una lesione traumatica da schiacciamento del midollo spinale della colonna dorsale.

Imaging intravitale di assonale Interazioni con Microglia e macrofagi in un mouse Dorsale colonna Crush Injury

Questo video mostra la procedura di craniotomia che consente l'imaging cronico dei neuroni nella corteccia retrospleniale del topo utilizzando la microscopia a due fotoni in vivo nella linea transgenica Thy1-GFP. Questo approccio è combinato con l'iniezione di virus adeno-associato che esprime mCherry nell'ippocampo dorsale. Queste tecniche consentono il monitoraggio a lungo termine della plasticità strutturale dipendente dall'esperienza in RSC.

Simultanea a due fotoni<em&gt; In Vivo</em&gt; Imaging di Synaptic ingressi e degli obiettivi postsinaptici nella corteccia mouse retrosplenial

Vi presentiamo una procedura di espulsione locale ottimizzata utilizzando una micro-pipetta di vetro e un metodo di imaging iperpilato a due fotoni veloce, che consente una misurazione precisa dei cambiamenti di diametro capillare e l'analisi della sua regolazione in tre dimensioni.

In Vivo microscopia bidimensionale bidimensionale per studiare le risposte vascolari mediante l'espulsione locale dell'ATP utilizzando una micropipetta di vetro

I cambiamenti morfologici si verificano nelle cellule fibroblaste immunitarie dopo l'attivazione e promuovono alterazioni nel reclutamento cellulare. Utilizzo dell'imaging a 2 fotoni in combinazione con una proteina specifica fibroblasta geneticamente ingegnerizzata 1 (FSP1)-cre; tdTomato- linea di mouse flossa-stop-floxed (TB/TB) e verde fluorescente etichettato lipopolysaccharide-FITC, possiamo illustrare l'assorbimento altamente specifico di lipopolissaccharide nei fibroblasti dermici e...

In Vivo a due colori immagini fotone di cellule reporter geneticamente taggate nella pelle

Scopri l'imaging a due fotoni intravitale per visualizzare i terminali assonali LTMR nella pelle della zampa anteriore del topo. Questo metodo consente studi ad alta risoluzione sullo sviluppo dei circuiti somatosensoriali in vivo.

Imaging a due fotoni intravitali della morfologia degli assoni sensoriali del tatto nella pelle del topo

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