Name: Metodi di costruzione per dispositivi microfluidici ibridi policarbonato-silicio (Video) | JoVE Journal
Uploaded: 2026-07-28T12:35:00.000+00:00
Duration: 8 min 18 s
Description: Scopri tre strategie di bonding a basso costo per dispositivi microfluidici ibridi policarbonato-silicio. Questo metodo permette di assemblare prototipi affidabili senza attrezzature specializzate in laboratori accademici.

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<ol><li>Yang, S. -M., Lv, S., Zhang, W., Cui, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microfluidic+point-of-care+%28POC%29+devices+in+early+diagnosis%3A+a+review+of+opportunities+and+challenges%5BTitle%5D)+AND+%22Sensors+%28Basel%29%22%5BJournal%5D)">Microfluidic point-of-care (POC) devices in early diagnosis: a review of opportunities and challenges.</a> Sensors (Basel). 22 (4), 1620(2022).</li>
<li>Kankaanpää, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+the+use+of+comprehensive+point-of-care+test+panel+to+conventional+laboratory+process+in+emergency+department%5BTitle%5D)+AND+%22BMC+Emerg+Med%22%5BJournal%5D)">Comparison of the use of comprehensive point-of-care test panel to conventional laboratory process in emergency department.</a> BMC Emerg Med. 18 (1), 43(2018).</li>
<li>Ingber, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+organs-on-chips+for+disease+modelling%2C+drug+development+and+personalized+medicine%5BTitle%5D)+AND+%22Nat+Rev+Genet%22%5BJournal%5D)">Human organs-on-chips for disease modelling, drug development and personalized medicine.</a> Nat Rev Genet. 23 (8), 467-491 (2022).</li>
<li>Baudoin, R., Griscom, L., Monge, M., Legallais, C., Leclerc, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+renal+microchip+for+in+vitro+distal+tubule+models%5BTitle%5D)+AND+%22Biotechnol+Prog%22%5BJournal%5D)">Development of a renal microchip for in vitro distal tubule models.</a> Biotechnol Prog. 23 (1), 124-131 (2007).</li>
<li>Spinelli, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Wearable+microfluidic+biosensors+with+haptic+feedback+for+continuous+monitoring+of+hydration+biomarkers+in+workers%5BTitle%5D)+AND+%22npj+Digit+Med%22%5BJournal%5D)">Wearable microfluidic biosensors with haptic feedback for continuous monitoring of hydration biomarkers in workers.</a> npj Digit Med. 8 (1), 76(2025).</li>
<li>Pour, S. R. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microfluidic-based+non-invasive+wearable+biosensors+for+real-time+monitoring+of+sweat+biomarkers%5BTitle%5D)+AND+%22Biosensors%22%5BJournal%5D)">Microfluidic-based non-invasive wearable biosensors for real-time monitoring of sweat biomarkers.</a> Biosensors. 14 (1), 29(2024).</li>
<li>S, A. D., P, S. P. A., Naveen, J., Khan, T., Khahro, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advancement+in+biomedical+implant+materials%E2%80%94a+mini+review%5BTitle%5D)+AND+%22Front+Bioeng+Biotechnol%22%5BJournal%5D)">Advancement in biomedical implant materials—a mini review.</a> Front Bioeng Biotechnol. 12, 1400918(2024).</li>
<li>Ranjan, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chitosan+with+PVC+polymer+for+biomedical+applications%3A+a+bibliometric+analysis%5BTitle%5D)+AND+%22Mater+Today+Proc%22%5BJournal%5D)">Chitosan with PVC polymer for biomedical applications: a bibliometric analysis.</a> Mater Today Proc. 81, 894-898 (2023).</li>
<li>Szydzik, C., Brazilek, R. J., Nesbitt, W. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+review+of+design+considerations+for+hemocompatibility+within+microfluidic+systems%5BTitle%5D)+AND+%22Semin+Thromb+Hemost%22%5BJournal%5D)">A review of design considerations for hemocompatibility within microfluidic systems.</a> Semin Thromb Hemost. 46 (5), 622-636 (2020).</li>
<li>Xu, Y., Yu, G., Nie, R., Wu, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microfluidic+systems+toward+blood+hemostasis+monitoring+and+thrombosis+diagnosis%3A+from+design+principles+to+micro%2Fnano+fabrication+technologies%5BTitle%5D)+AND+%22VIEW%22%5BJournal%5D)">Microfluidic systems toward blood hemostasis monitoring and thrombosis diagnosis: from design principles to micro/nano fabrication technologies.</a> VIEW. 3 (1), 20200183(2022).</li>
<li>Blauvelt, D. G., Abada, E. N., Oishi, P., Roy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+extracorporeal+membrane+oxygenator+design+for+artificial+placenta+technology%5BTitle%5D)+AND+%22Artif+Organs%22%5BJournal%5D)">Advances in extracorporeal membrane oxygenator design for artificial placenta technology.</a> Artif Organs. 45 (3), 205-221 (2021).</li>
<li>Matellan, C., Del Río Hernández, A. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cost-effective+rapid+prototyping+and+assembly+of+poly%28methyl+methacrylate%29+microfluidic+devices%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Cost-effective rapid prototyping and assembly of poly(methyl methacrylate) microfluidic devices.</a> Sci Rep. 8 (1), 6971(2018).</li>
<li>Yu, H., Chong, Z. Z., Tor, S. B., Liu, E., Loh, N. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Low+temperature+and+deformation-free+bonding+of+PMMA+microfluidic+devices+with+stable+hydrophilicity+via+oxygen+plasma+treatment+and+PVA+coating%5BTitle%5D)+AND+%22RSC+Adv%22%5BJournal%5D)">Low temperature and deformation-free bonding of PMMA microfluidic devices with stable hydrophilicity via oxygen plasma treatment and PVA coating.</a> RSC Adv. 5 (11), 8377-8388 (2015).</li>
<li>Testa, G., Persichetti, G., Sarro, P. M., Bernini, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+hybrid+silicon-PDMS+optofluidic+platform+for+sensing+applications%5BTitle%5D)+AND+%22Biomed+Opt+Express%22%5BJournal%5D)">A hybrid silicon-PDMS optofluidic platform for sensing applications.</a> Biomed Opt Express. 5 (2), 417-425 (2014).</li>
<li>Toniolo, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Efficient+and+reliable+encapsulation+for+perovskite%2Fsilicon+tandem+solar+modules%5BTitle%5D)+AND+%22Nanoscale%22%5BJournal%5D)">Efficient and reliable encapsulation for perovskite/silicon tandem solar modules.</a> Nanoscale. 15 (42), 16984-16991 (2023).</li>
<li>Chiang, C. -C., Immanuel, P. N., Chiu, Y. -H., Huang, S. -J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heterogeneous+bonding+of+PMMA+and+double-sided+polished+silicon+wafers+through+H2O+plasma+treatment+for+microfluidic+devices%5BTitle%5D)+AND+%22Coatings%22%5BJournal%5D)">Heterogeneous bonding of PMMA and double-sided polished silicon wafers through H2O plasma treatment for microfluidic devices.</a> Coatings. 11 (5), 580(2021).</li>
<li>Moyer, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Surgical+refinement+of+silicon+nanopore+membrane+devices+for+implantable+kidney+replacement+therapy%3A+7-year+experience+across+90+prototypes%3A+SA-PO557%5BTitle%5D)+AND+%22J+Am+Soc+Nephrol%22%5BJournal%5D)">Surgical refinement of silicon nanopore membrane devices for implantable kidney replacement therapy: 7-year experience across 90 prototypes: SA-PO557.</a> J Am Soc Nephrol. 35 (10S), (2024).</li>
<li>Santandreu, A. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+human+islet+function+in+a+convection-driven+intravascular+bioartificial+pancreas%5BTitle%5D)+AND+%22Bioeng+Transl+Med%22%5BJournal%5D)">Characterization of human islet function in a convection-driven intravascular bioartificial pancreas.</a> Bioeng Transl Med. 8 (2), e10444(2023).</li>
<li>Kim, E. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Feasibility+of+an+implantable+bioreactor+for+renal+cell+therapy+using+silicon+nanopore+membranes%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes.</a> Nat Commun. 14 (1), 4890(2023).</li>
<li>Dharia, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Silicon+micropore-based+parallel+plate+membrane+oxygenator%5BTitle%5D)+AND+%22Artif+Organs%22%5BJournal%5D)">Silicon micropore-based parallel plate membrane oxygenator.</a> Artif Organs. 42 (2), 166-173 (2018).</li>
<li>Blauvelt, D. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+silicon+membrane+microfluidic+oxygenator+for+use+as+an+artificial+placenta+with+minimal+anticoagulation%5BTitle%5D)+AND+%22Bioeng+Transl+Med%22%5BJournal%5D)">A silicon membrane microfluidic oxygenator for use as an artificial placenta with minimal anticoagulation.</a> Bioeng Transl Med. , e70037(2025).</li>
<li>Aralekallu, S., Boddula, R., Singh, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+glass-based+microfluidic+devices%3A+a+review+on+its+fabrication+and+biologic+applications%5BTitle%5D)+AND+%22Mater+Des%22%5BJournal%5D)">Development of glass-based microfluidic devices: a review on its fabrication and biologic applications.</a> Mater Des. 225, 111517(2023).</li>
<li>Carvell, T., Burgoyne, P., Fraser, A. R., Bridle, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Categorising+hybrid+material+microfluidic+devices%5BTitle%5D)+AND+%22Front+Lab+Chip+Technol%22%5BJournal%5D)">Categorising hybrid material microfluidic devices.</a> Front Lab Chip Technol. 3, 1412290(2024).</li>
<li>Yadavali, S., Jeong, H. -H., Lee, D., Issadore, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Silicon+and+glass+very+large+scale+microfluidic+droplet+integration+for+terascale+generation+of+polymer+microparticles%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">Silicon and glass very large scale microfluidic droplet integration for terascale generation of polymer microparticles.</a> Nat Commun. 9 (1), 1222(2018).</li>
<li>Thompson, A. J., Ma, L. J., Plegue, T. J., Potkay, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Design+analysis+and+optimization+of+a+single-layer+PDMS+microfluidic+artificial+lung%5BTitle%5D)+AND+%22IEEE+Trans+Biomed+Eng%22%5BJournal%5D)">Design analysis and optimization of a single-layer PDMS microfluidic artificial lung.</a> IEEE Trans Biomed Eng. 66 (4), 1082-1093 (2019).</li>
<li>Tony, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+additive+manufacturing+approach+to+polydimethylsiloxane+%28PDMS%29+microfluidic+devices%3A+review+and+future+directions%5BTitle%5D)+AND+%22Polymers%22%5BJournal%5D)">The additive manufacturing approach to polydimethylsiloxane (PDMS) microfluidic devices: review and future directions.</a> Polymers. 15 (8), 1926(2023).</li>
<li>Gonçalves, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Polydimethylsiloxane+surface+modification+of+microfluidic+devices+for+blood+plasma+separation%5BTitle%5D)+AND+%22Polymers%22%5BJournal%5D)">Polydimethylsiloxane surface modification of microfluidic devices for blood plasma separation.</a> Polymers. 16 (10), 1416(2024).</li>
<li>Khoo, H., Allen, W. S., Arroyo-Currás, N., Hur, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+prototyping+of+thermoplastic+microfluidic+devices+via+SLA+3D+printing%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Rapid prototyping of thermoplastic microfluidic devices via SLA 3D printing.</a> Sci Rep. 14 (1), 17646(2024).</li>
<li>Gencturk, E., Mutlu, S., Ulgen, K. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+microfluidic+devices+made+from+thermoplastics+used+in+cell+biology+and+analyses%5BTitle%5D)+AND+%22Biomicrofluidics%22%5BJournal%5D)">Advances in microfluidic devices made from thermoplastics used in cell biology and analyses.</a> Biomicrofluidics. 11 (5), 051502(2017).</li>
<li>Islam, M. M., Loewen, A., Allen, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simple%2C+low-cost+fabrication+of+acrylic+based+droplet+microfluidics+and+its+use+to+generate+DNA-coated+particles%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Simple, low-cost fabrication of acrylic based droplet microfluidics and its use to generate DNA-coated particles.</a> Sci Rep. 8 (1), 8763(2018).</li>
<li>Giri, K., Tsao, C. -W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+advances+in+thermoplastic+microfluidic+bonding%5BTitle%5D)+AND+%22Micromachines%22%5BJournal%5D)">Recent advances in thermoplastic microfluidic bonding.</a> Micromachines. 13 (3), 486(2022).</li>
<li>Wan, A. M. D., Moore, T. A., Young, E. W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Solvent+bonding+for+fabrication+of+PMMA+and+COP+microfluidic+devices%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Solvent bonding for fabrication of PMMA and COP microfluidic devices.</a> J Vis Exp. (119), e55175(2017).</li>
<li>Ogończyk, D., Węgrzyn, J., Jankowski, P., Dąbrowski, B., Garstecki, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bonding+of+microfluidic+devices+fabricated+in+polycarbonate%5BTitle%5D)+AND+%22Lab+Chip%22%5BJournal%5D)">Bonding of microfluidic devices fabricated in polycarbonate.</a> Lab Chip. 10 (10), 1324-1327 (2010).</li>
<li>Ahmed, M. A. M., Jurczak, K. M., Lynn, N. S., Mulder, J. -P. S. H., Verpoorte, E. M. J., Nagelkerke, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+prototyping+of+PMMA-based+microfluidic+spheroid-on-a-chip+models+using+micromilling+and+vapour-assisted+thermal+bonding%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Rapid prototyping of PMMA-based microfluidic spheroid-on-a-chip models using micromilling and vapour-assisted thermal bonding.</a> Sci Rep. 14 (1), 2831(2024).</li>
<li>Hassanpour-Tamrin, S., Sanati-Nezhad, A., Sen, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simple+and+low-cost+approach+for+irreversible+bonding+of+polymethylmethacrylate+and+polydimethylsiloxane+at+room+temperature+for+high-pressure+hybrid+microfluidics%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">A simple and low-cost approach for irreversible bonding of polymethylmethacrylate and polydimethylsiloxane at room temperature for high-pressure hybrid microfluidics.</a> Sci Rep. 11 (1), 4821(2021).</li>
<li>Zhao, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Plastic+polymers+for+efficient+DNA+microarray+hybridization%3A+application+to+microbiological+diagnostics%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">Plastic polymers for efficient DNA microarray hybridization: application to microbiological diagnostics.</a> J Clin Microbiol. 46 (11), 3752-3758 (2008).</li>
<li>Nielsen, J. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microfluidics%3A+innovations+in+materials+and+their+fabrication+and+functionalization%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Microfluidics: innovations in materials and their fabrication and functionalization.</a> Anal Chem. 92 (1), 150-168 (2020).</li>
<li>Washburn, A. L., Gunn, L. C., Bailey, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Label-free+quantitation+of+a+cancer+biomarker+in+complex+media+using+silicon+photonic+microring+resonators%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Label-free quantitation of a cancer biomarker in complex media using silicon photonic microring resonators.</a> Anal Chem. 81 (22), 9499-9506 (2009).</li>
<li>Kojic, S. P., Stojanovic, G. M., Radonic, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Novel+cost-effective+microfluidic+chip+based+on+hybrid+fabrication+and+its+comprehensive+characterization%5BTitle%5D)+AND+%22Sensors+%28Basel%29%22%5BJournal%5D)">Novel cost-effective microfluidic chip based on hybrid fabrication and its comprehensive characterization.</a> Sensors (Basel). 19 (7), 1719(2019).</li>
<li>Boursier, M., Bayon, Y., Negrell, C., Pinaud, J., Caillol, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biocompatible+glues%3A+recent+progress+and+emerging+frontiers+in+surgical+adhesion%5BTitle%5D)+AND+%22Polymers%22%5BJournal%5D)">Biocompatible glues: recent progress and emerging frontiers in surgical adhesion.</a> Polymers. 17 (13), 1749(2025).</li>
<li>Higgins, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Engineering+a+silicon+membrane+oxygenator+for+an+artificial+placenta%5BTitle%5D)+AND+%22eScholarship%22%5BJournal%5D)">Engineering a silicon membrane oxygenator for an artificial placenta.</a> eScholarship. , (2025).</li>
<li>Wei, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+prototyping+of+multi-functional+and+biocompatible+Parafilm%C2%AE-based+microfluidic+devices+by+laser+ablation+and+thermal+bonding%5BTitle%5D)+AND+%22Micromachines%22%5BJournal%5D)">Rapid prototyping of multi-functional and biocompatible Parafilm®-based microfluidic devices by laser ablation and thermal bonding.</a> Micromachines. 14 (3), 656(2023).</li>
<li>Madadi, M., Madadi, A., Zareifar, R., Nikfarjam, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simple+solvent-assisted+method+for+thermal+bonding+of+large-surface%2C+multilayer+PMMA+microfluidic+devices%5BTitle%5D)+AND+%22Sens+Actuators+A+Phys%22%5BJournal%5D)">A simple solvent-assisted method for thermal bonding of large-surface, multilayer PMMA microfluidic devices.</a> Sens Actuators A Phys. 349, 114077(2023).</li>
<li>Riegger, L., Strohmeier, O., Faltin, B., Zengerle, R., Koltay, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adhesive+bonding+of+microfluidic+chips%3A+influence+of+process+parameters%5BTitle%5D)+AND+%22J+Micromech+Microeng%22%5BJournal%5D)">Adhesive bonding of microfluidic chips: influence of process parameters.</a> J Micromech Microeng. 20 (8), 087003(2010).</li>
<li>Jung, B. -J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Surface+functionalization+and+bonding+of+chemically+inert+parylene+microfluidics+using+Parylene-A+adhesive+layer%5BTitle%5D)+AND+%22BioChip+J%22%5BJournal%5D)">Surface functionalization and bonding of chemically inert parylene microfluidics using Parylene-A adhesive layer.</a> BioChip J. 16 (2), 168-174 (2022).</li>
</ol>

<table><tbody><tr><td>.040" (1.00 mm) Buna-N 70 O-Ring Cord Stock</td><td>The O-Ring Store</td><td>N70.040</td><td>Guarnizione da 1 mm utilizzata nell'assemblaggio; diametro regolabile per design</td></tr><tr><td>18-8 Stainless Steel Dowel Pin</td><td>McMaster</td><td>90144A876</td><td>Usato per l'allineamento in LDPE e incollaggio con solvente</td></tr><tr><td>18-8 Stainless Steel Washer</td><td>McMaster</td><td>92141A006</td><td>Usato nell'assemblaggio della guarnizione</td></tr><tr><td>316 Stainless Steel Ultra-Low-Profile Socket Head Screw</td><td>McMaster</td><td>91223A315</td><td>Viti utilizzate nell'assemblaggio della guarnizione</td></tr><tr><td>CAD Software</td><td>OnShape o SolidWorks (o equivalente)</td><td>Usato per il design del dispositivo</td><td></td></tr><tr><td>Desktop CO2 Laser Cutter</td><td>Universal Laser Systems (o equivalente)</td><td>Usato per il taglio di fogli LDPE e nastro adesivo</td><td></td></tr><tr><td>Dichloromethane, anhydrous, 99.7+%</td><td>ThermoFisher</td><td>047352.M1</td><td>Solvente usato per l'incollaggio con solvente</td></tr><tr><td>Digital Torque Screwdriver</td><td>Checkline</td><td>TSD-400</td><td>Usato per applicare una compressione controllata nell'assemblaggio della guarnizione</td></tr><tr><td>Epoxy Resin, two-part</td><td>Epoxy Technology (o equivalente)</td><td>MED-302</td><td>Usato per l'incollaggio del silicone al policarbonato</td></tr><tr><td>Hex Nut, Low-Strength Steel</td><td>McMaster</td><td>90480A007</td><td>Usato con viti per la compressione della guarnizione</td></tr><tr><td>Low-Density Polyethylene (LDPE) Film</td><td>McMaster</td><td>8593K71</td><td>Foglio LDPE da 100-200 µm per l'incollaggio termico</td></tr><tr><td>Microfluidic Diagnostic Adhesive Tape</td><td>3M</td><td>9965; 9972A</td><td>Usato come strato di mascheratura temporaneo durante l'incollaggio con solvente</td></tr><tr><td>Peristaltic Pump Tubing (Size 16 equivalent)</td><td>Masterflex (o equivalente)</td><td>Compatibile con la pompa rotante usata nei test</td><td></td></tr><tr><td>Polycarbonate Housing (machined)</td><td>Xometry (o equivalente)</td><td>Strati di dispositivo in policarbonato lavorati a CNC</td><td></td></tr><tr><td>Pressure Sensor</td><td></td><td></td><td>Usato per misurare le pressioni di ingresso e di uscita</td></tr><tr><td>Programmable Laboratory Oven</td><td></td><td></td><td>Capace di mantenere 145 °C per l'incollaggio LDPE</td></tr><tr><td>Roller Pump</td><td></td><td></td><td>Usato per test di pressione e flusso</td></tr><tr><td>Silicon Substrate (solid chip)</td><td>MicroDicing (o equivalente)</td><td>Usato come componente siliconico modello</td><td></td></tr><tr><td>Stainless Steel Dispensing Needles with Luer Lock</td><td>McMaster</td><td>75165A688</td><td>Usate per l'erogazione di epossidico e solvente</td></tr></tbody></table>

metodi di costruzione per dispositivi microfluidici ibridi policarbonato-silicio

Construction Methods for Hybrid Polycarbonate-Silicon Microfluidic Devices

Metodi di costruzione per dispositivi microfluidici ibridi policarbonato-silicio

Hybrid microfluidic devices combining thermoplastics with rigid substrates, such as silicon and glass, enable advanced biological applications but are ...

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Bioengineering

Bioingegneria

Dispositivi microfluidici può essere utilizzato per visualizzare complessi processi naturali in tempo reale e alle scale fisico appropriato. Abbiamo sviluppato un semplice dispositivo a microfluidi che simula le caratteristiche principali di mezzi porosi naturali per studiare la crescita e il trasporto di batteri nel sottosuolo.

Finestra su un micromondo: Sistemi Microfluidic semplice per studiare il trasporto microbica in mezzi porosi

Fonte: Kurz, D. L., et al. Piattaforma microfluidica per studiare il bioclogging in mezzi porosi. J. Vis. Esp. (2022)Il video dimostra l'uso di un dispositivo microfluidico per studiare la formazione del biofilm. Viene introdotta una sospensione batterica nel microcanale, seguita da un'incubazione per permettere lo sviluppo del biofilm. Il flusso viene quindi ripreso e il monitoraggio della pressione insieme all'imaging viene utilizzato per rilevare la formazione di biofilm e l'ostruzione dei...

Un dispositivo microfluidico per l'imaging in tempo reale e il monitoraggio della pressione durante la formazione del biofilm

I dispositivi microfluidici multistrato spesso comportano la fabbricazione di stampi master con geometrie complesse per la funzionalità. Questo articolo presenta un protocollo completo per la fotolitografia multi-step con valvole e funzioni ad altezza variabile adattabili a qualsiasi applicazione. A titolo dimostrativo, fabbrichiamo un generatore di goccioline microfluidiche in grado di produrre perle di idrogel.

Multi-step altezza variabile fotolitografia per Valvolati multistrato microfluidici Devices

Il controllo dei gradienti chimici e di ossigeno è essenziale per le colture cellulari. Questo articolo riporta un dispositivo microfluidico in polidimetilsilossano-policarbonato (PDMS-PC) in grado di generare in modo affidabile combinazioni di gradienti chimici e di ossigeno per studi di migrazione cellulare, che può essere praticamente utilizzato nei laboratori biologici senza strumentazione sofisticata.

Polidimetilsilossano-policarbonato dispositivi microfluidici per Cell Migration Studies Sotto perpendicolare chimica e gradienti di ossigeno

Abbiamo dimostrato una piattaforma microfluidica con una rete elettrodo superficie integrato che combina rilevamento di impulsi resistivo (RPS) con code division multiple access (CDMA), per multiplare rilevazione e dimensionamento delle particelle a più canali microfluidici.

Piattaforma microfluidica con multiplex di rilevamento elettronico per spaziale monitoraggio di particelle

Un protocollo per la fabbricazione di dispositivi microfluidici plastica con trasparente vista-porte per l'imaging di luce visibile e dell'infrarosso è descritto.

Procedura litografica morbido per la produzione di dispositivi microfluidici plastica con vista-porte trasparente alla luce visibile e infrarossa

Diversi metodi sono disponibili per la realizzazione di canali di sezioni non rettangolari incorporati in dispositivi microfluidici polidimetilsilossano. La maggior parte di essi coinvolgono produzione multistep e vasto allineamento. In questa carta, un approccio One-Step è segnalato per la realizzazione di canali microfluidici di diverse sezioni trasversali geometriche di polidimetilsilossano sequenziale acquaforte bagnata.

One-Step approccio a fabbricare canali microfluidici Polydimethylsiloxane di diverse sezioni geometriche da processi sequenziali acquaforte bagnata

Qui presentiamo un protocollo per progettare e fabbricare dispositivi microfluidici personalizzati con un minimo investimento finanziario e temporale. L'obiettivo è facilitare l'adozione di tecnologie microfluidiche nei laboratori di ricerca biomedica e negli ambienti educativi.

Rapida fabbricazione di dispositivi microfluidici personalizzati per applicazioni di ricerca e didattiche

Il presente protocollo descrive una piattaforma microfluidica per studiare lo sviluppo di biofilm in mezzi porosi quasi-2D combinando l'imaging al microscopio ad alta risoluzione con misurazioni simultanee della differenza di pressione. La piattaforma quantifica l'influenza della dimensione dei pori e delle portate del fluido nei mezzi porosi sul biointasamento.

Una piattaforma microfluidica per studiare il bioclogging in mezzi porosi

Questo articolo descrive i protocolli per la fabbricazione di microgel di gelatina metacriloile ad alta produttività utilizzando dispositivi microfluidici, la conversione di microgel in polvere risostenibile (micro-aerogel), l'assemblaggio chimico di microgel per formare scaffold di idrogel granulare e lo sviluppo di bioink granulari di idrogel con microporosità conservata per il bioprinting 3D.

Scaffold di idrogel granulare di gelatina metacrilico: fabbricazione di microgel ad alta produttività, liofilizzazione, assemblaggio chimico e bioprinting 3D

Qui viene presentata la fabbricazione di un dispositivo a doppio strato basato su polidimetilsilossano (PDMS) per la produzione di librerie combinatorie in emulsioni acqua-in-olio (tappi). L'hardware e il software necessari per automatizzare la produzione di tappi sono dettagliati nel protocollo e viene dimostrata anche la produzione di una libreria quantitativa di tappi fluorescenti.

Dispositivo microfluidico a doppio strato per la produzione di tappi combinatori

Scopri tre strategie di bonding a basso costo per dispositivi microfluidici ibridi policarbonato-silicio. Questo metodo permette di assemblare prototipi affidabili senza attrezzature specializzate in laboratori accademici.

Metodi di costruzione per dispositivi microfluidici ibridi policarbonato-silicio

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