Profilazione fenotipica dei fenotipi comportamentali simili a Parkinson indotti da MPTP nelle larve di pesce zebra, basata su un esperimento comportamentale
1.8K visualizzazioni
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10:33 min.
•
15 maggio 2026
DOI :10.3791/70041-v
15 maggio 2026
•1.8K visualizzazioni
Zhuang Miao1 , Jiying Bi1 , Jie Zhang2,3 , Min Tian4 , Zhuoran Yan4 , Ruonan Duan4 , Ning Wang2,3 , Xiang Li1,5,6
1Department of Medicinal Chemistry, State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Key Laboratory of Chemical Biology (Ministry of Education), NMPA Key Laboratory for Technology Research and Evaluation of Drug Products, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 2College of Information Engineering, Ningde Normal University, 3Digital Fujian Research Institute of Big Data for Elderly Rehabilitation and Nursing, 4Department of Neurology, Qilu Hospital Cheeloo College of Medicine, Shandong University, 5Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, 6Jinan Microecological Biomedicine Shandong Laboratory
Questo protocollo stabilisce un paradigma di fenotipizzazione comportamentale nelle larve di pesce zebra trattate con MPTP per valutare i deficit motori e non motori simili alla malattia di Parkinson.
Chapters in this video
0:00
Introduction
0:26
Zebrafish Embryo Preparation for Behavioral Assessment
1:44
Sleep‑Wake Behavioral Analysis of Zebrafish Larvae Using Zebrabox System
3:39
Assessment of Photomotor Response
4:35
Assessment of Thigmotaxis
5:23
Evaluation of Locomotor Activity
5:52
Startle Response Test
7:18
Light‑Dark Challenge Assay
8:24
Results
9:54
Conclusion
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