Name: Modello di ratto canulato con dotto linfatico cervicale per la valutazione del trasporto linfatico dalla testa e dal cervello (Video) | JoVE Journal
Uploaded: 2026-03-10T13:10:00.000+00:00
Duration: 8 min 3 s
Description: Impara la cannulazione del dotto linfatico cervicale profondo nel ratto per campionare direttamente la linfa cerebrale. Questo metodo consente di quantificare il trasporto linfatico e lo svuotamento di sostanze dal sistema nervoso centrale.

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<li>Khang, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intrathecal+delivery+of+nanoparticle+PARP+inhibitor+to+the+cerebrospinal+fluid+for+the+treatment+of+metastatic+medulloblastoma%5BTitle%5D)+AND+%22Sci+Transl+Med%22%5BJournal%5D)">Intrathecal delivery of nanoparticle PARP inhibitor to the cerebrospinal fluid for the treatment of metastatic medulloblastoma.</a> Sci Transl Med. 15 (720), eadi1617(2023).</li>
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<li>Yan, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lymphatic+clearance+is+the+main+drainage+route+of+lamotrigine-loaded+micelles+following+delivery+to+the+brain%5BTitle%5D)+AND+%22J+Pharm+Pharmacol%22%5BJournal%5D)">Lymphatic clearance is the main drainage route of lamotrigine-loaded micelles following delivery to the brain.</a> J Pharm Pharmacol. 71 (10), 1488-1496 (2019).</li>
<li>Ringstad, G., Eide, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Glymphatic-lymphatic+coupling%3A+assessment+of+the+evidence+from+magnetic+resonance+imaging+of+humans%5BTitle%5D)+AND+%22Cell+Mol+Life+Sci%22%5BJournal%5D)">Glymphatic-lymphatic coupling: assessment of the evidence from magnetic resonance imaging of humans.</a> Cell Mol Life Sci. 81 (1), 131(2024).</li>
<li>Pan, W. R., Suami, H., Taylor, G. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lymphatic+drainage+of+the+superficial+tissues+of+the+head+and+neck%3A+anatomical+study+and+clinical+implications%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Lymphatic drainage of the superficial tissues of the head and neck: anatomical study and clinical implications.</a> Plast Reconstr Surg. 121 (5), 1614-1624 (2008).</li>
<li>Bou-Assaly, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+forgotten+lymph+nodes%3A+review+of+the+superficial+head+and+neck+lymphatic+system%5BTitle%5D)+AND+%22Radiol+Imaging%22%5BJournal%5D)">The forgotten lymph nodes: review of the superficial head and neck lymphatic system.</a> Radiol Imaging. 1 (1), 9-13 (2016).</li>
<li>Yağmurlu, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Anatomical+features+of+the+deep+cervical+lymphatic+system+and+intrajugular+lymphatic+vessels+in+humans%5BTitle%5D)+AND+%22Brain+Sci%22%5BJournal%5D)">Anatomical features of the deep cervical lymphatic system and intrajugular lymphatic vessels in humans.</a> Brain Sci. 10 (12), 953(2020).</li>
<li>Suami, H., Scaglioni, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lymphatic+territories+%28lymphosomes%29+in+the+rat%3A+an+anatomical+study+for+future+lymphatic+research%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Lymphatic territories (lymphosomes) in the rat: an anatomical study for future lymphatic research.</a> Plast Reconstr Surg. 140 (5), 945-951 (2017).</li>
<li>Zawieja, D. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lymphatic+cannulation+for+lymph+sampling+and+molecular+delivery%5BTitle%5D)+AND+%22J+Immunol%22%5BJournal%5D)">Lymphatic cannulation for lymph sampling and molecular delivery.</a> J Immunol. 203 (8), 2339-2350 (2019).</li>
<li>Hoang, T. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantifying+the+lymphatic+transport+of+model+therapeutics+from+the+brain+in+rats%5BTitle%5D)+AND+%22Mol+Pharm%22%5BJournal%5D)">Quantifying the lymphatic transport of model therapeutics from the brain in rats.</a> Mol Pharm. 21 (5), 2473-2483 (2024).</li>
<li>Hoang, T. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+neuroinflammation+alters+the+transport+of+a+model+therapeutic+protein+from+the+brain+into+lymph+and+blood%5BTitle%5D)+AND+%22Mol+Pharm%22%5BJournal%5D)">Acute neuroinflammation alters the transport of a model therapeutic protein from the brain into lymph and blood.</a> Mol Pharm. 21 (10), 5138-5149 (2024).</li>
<li>Maloveska, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dynamics+of+Evans+blue+clearance+from+cerebrospinal+fluid+into+meningeal+lymphatic+vessels+and+deep+cervical+lymph+nodes%5BTitle%5D)+AND+%22Neurol+Res%22%5BJournal%5D)">Dynamics of Evans blue clearance from cerebrospinal fluid into meningeal lymphatic vessels and deep cervical lymph nodes.</a> Neurol Res. 40 (5), 372-380 (2018).</li>
<li>Trevaskis, N. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+mesenteric+lymph+duct+cannulated+rat+model%3A+application+to+the+assessment+of+intestinal+lymphatic+drug+transport%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">The mesenteric lymph duct cannulated rat model: application to the assessment of intestinal lymphatic drug transport.</a> J Vis Exp. (97), e52389(2015).</li>
<li>Gakuba, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((General+anesthesia+inhibits+the+activity+of+the+glymphatic+system%5BTitle%5D)+AND+%22Theranostics%22%5BJournal%5D)">General anesthesia inhibits the activity of the glymphatic system.</a> Theranostics. 8 (3), 710-722 (2018).</li>
<li>Miao, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Brain+clearance+is+reduced+during+sleep+and+anesthesia%5BTitle%5D)+AND+%22Nat+Neurosci%22%5BJournal%5D)">Brain clearance is reduced during sleep and anesthesia.</a> Nat Neurosci. 27 (6), 1046-1050 (2024).</li>
<li>Bachmann, S. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+effects+of+anaesthesia+on+the+contractility+of+lymphatic+vessels+in+vivo%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">Differential effects of anaesthesia on the contractility of lymphatic vessels in vivo.</a> J Physiol. 597 (11), 2841-2852 (2019).</li>
<li>Ruscic, K. J., Padera, T. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Silence+of+the+lymphs%3A+some+anaesthetic+regimens+inhibit+lymphatic+pumping%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">Silence of the lymphs: some anaesthetic regimens inhibit lymphatic pumping.</a> J Physiol. 597 (11), 2827-2828 (2019).</li>
<li>Dahan, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+effect+of+general+anesthesia+on+the+intestinal+lymphatic+transport+of+lipophilic+drugs%3A+comparison+between+anesthetized+and+freely+moving+conscious+rat+models%5BTitle%5D)+AND+%22Eur+J+Pharm+Sci%22%5BJournal%5D)">The effect of general anesthesia on the intestinal lymphatic transport of lipophilic drugs: comparison between anesthetized and freely moving conscious rat models.</a> Eur J Pharm Sci. 32 (4), 367-374 (2007).</li>
<li>de Jong, W. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Long-term+cannulation+of+the+vena+cava+of+rats+for+blood+sampling%3A+local+and+systemic+effects+observed+by+histopathology+after+six+weeks+of+cannulation%5BTitle%5D)+AND+%22Lab+Anim%22%5BJournal%5D)">Long-term cannulation of the vena cava of rats for blood sampling: local and systemic effects observed by histopathology after six weeks of cannulation.</a> Lab Anim. 35 (3), 243-248 (2001).</li>
</ol>

<table><tbody><tr><td>Ago calibro 30</td><td>Yushou Health</td><td></td><td>Utilizzata per collegare l'estremità prossimale della cannula (0503) con la siringa per eparina</td></tr><tr><td>Dispensatore di anestesia con vaporizzatore</td><td>YYUN Instrument Company</td><td></td><td></td></tr><tr><td>Pinze smussate</td><td>Scienze della Vita RWD</td><td>F22002-10&nbsp;</td><td></td></tr><tr><td>Cotton bob</td><td>Biotecnologia Qingdao Shultz</td><td>MQ-02</td><td>Usata per tenere temporaneamente il tessuto muscolare in posizione</td></tr><tr><td>Assorbente chirurgico riscaldato</td><td>Shanghai Yiheng Instruments</td><td></td><td>Dimensione 15 e tempi; 20 cm</td></tr><tr><td>Eparina sodica</td><td>Tecnologia Biochimica di Shanghai Aladdin</td><td>H123383</td><td>Diluizione necessaria prima dell'uso</td></tr><tr><td>Siringa per insulina (1 mL)</td><td>Yushou Health</td><td>U100-1ML</td><td>Usato solo per il pre-rivestimento dell'eparina</td></tr><tr><td>Soluzione di isoflurano per l'inalazione</td><td>Scienze della Vita RWD</td><td>R510-22-10</td><td>Usato per l'anestesia tramite vaporizzatore</td></tr><tr><td>Penne marcatrici</td><td>Qualsiasi marca</td><td>N/A</td><td></td></tr><tr><td>Tubo microcentrifuga (1,5 mL)</td><td>Attrezzature sperimentali Nantong Hairui</td><td>HR10003</td><td>Utilizzato per la raccolta della linfa</td></tr><tr><td>Micro-forbici</td><td>Scienze della Vita RWD</td><td>GD234BA0007925</td><td></td></tr><tr><td>Sutura chirurgica in nylon&nbsp;</td><td>Shanghai Yiheng Instruments</td><td>4-0 35CM</td><td></td></tr><tr><td>Pipetta (regolabile a volume, intervallo da 5 a 50 e mu; L)</td><td>Dragon Laboratory Instruments Limited</td><td>7030301006</td><td>Utilizzato solo per misurare adesivi a tessuto</td></tr><tr><td>Punte per pipette&nbsp;</td><td>Biosharp Scienze della Vita</td><td>BS-10-T</td><td>Usato solo per il trasferimento di adesivo tissutale, senza volume specifico richiesto</td></tr><tr><td>Cannula in polieiilene (PE) 0,5 mm di diametro diametrale, 0,3 mm diametro diametrale (0503)</td><td>AniLab Strumenti Scientifici (Ningbo)</td><td>PE-0503</td><td></td></tr><tr><td>Cannula in polieiilene (PE) 0,6 mm di diametro diametrale, 0,5 mm diametro diametrale (0605)</td><td>AniLab Strumenti Scientifici (Ningbo)</td><td>PE-0605</td><td></td></tr><tr><td>Lamette di rasoio</td><td>Gillette Shanghai</td><td>Q31/0115000301C004</td><td>Usato solo per la rasatura del pelo</td></tr><tr><td>Elastico</td><td>Qualsiasi marca</td><td>N/A</td><td>Usato per trattenere gli arti anteriori dei ratti</td></tr><tr><td>Sovrano</td><td>Qualsiasi marca</td><td>N/A</td><td></td></tr><tr><td>Lame scapel (#23)</td><td>Scienze della Vita RWD</td><td>S31010-01</td><td></td></tr><tr><td>Soluzione di cloruro di sodio (0,9%) per infusione</td><td>Shijiazhuang No. 4 Pharmaceutica</td><td>H20066533 (numero di approvazione)</td><td>Usato solo per risciacquare pelli</td></tr><tr><td>Sprague-Dawley Rats</td><td>Tecnologia Animale di Laboratorio del Fiume Vitale di Pechino</td><td>101</td><td>Peso da 220 a 250 grammi, senza requisito di età</td></tr><tr><td>Luce chirurgica</td><td>Pechino Heo Bio-Tech</td><td>XHA011-1</td><td></td></tr><tr><td>Microscopio chirurgico</td><td>Tecnologia Optoelettronica Cewei di Shanghai</td><td>SMZ755T</td><td>Microscopio dissezionale senza palco meccanico, può essere equipaggiato con un supporto retrattile</td></tr><tr><td>Adesivo tissulare</td><td>3M Vetbond</td><td>1469SB</td><td>Ingrediente principale cianoacrilato &nbsp;</td></tr><tr><td>Espansore tissutale (Colibri)</td><td>Scienze della Vita RWD</td><td>R22029-04</td><td>Intervallo di espansione 20mm/4cm</td></tr></tbody></table>

modello di ratto canulato con dotto linfatico cervicale per la valutazione del trasporto linfatico dalla testa e dal cervello

Cervical Lymph Duct-Cannulated Rat Model for Assessing Lymphatic Transport from the Head and Brain

Modello di ratto canulato con dotto linfatico cervicale per la valutazione del trasporto linfatico dalla testa e dal cervello

Lymph flows from the central nervous system (CNS) via a series of lymphatic vessels (LVs) and lymph nodes that converge at the cervical lymph ducts. In ...

cervical-lymph-duct-cannulated-rat-model-for-assessing-lymphatic

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Immunology and Infection

Immunologia e Infezioni

Questo video mostra una tecnica per incannulare il dotto linfatico mesenterico nei ratti. Questo metodo consente l'accesso diretto all'intero volume di componenti presenti nel fluido linfatico.

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Questo video mostra l'incannulamento intraduodenale in un modello di ratto. Dopo aver anestetizzato il ratto, si localizza il duodeno e si crea una piccola puntura. Una cannula a forma di J con una punta smussata viene inserita e fissata con cura e l'incisione viene chiusa. Il modello di ratto cannulato consente l'infusione intra-intestinale per studi di immunopatologia.

Incannulamento intraduodenale in un modello di ratto per studi di immunopatologia

In questo video, dimostriamo una procedura chirurgica per incannulare l'arteria carotide sinistra in un modello di ratto.

Incannulamento dell'arteria carotide in un modello di ratto

Qui descriviamo una tecnica per incannulare il dotto linfatico mesenterico nei ratti che consente la quantificazione del trasporto di lipidi e farmaci attraverso il sistema linfatico dopo il parto intestinale. La tecnica può essere adattata per valutare le concentrazioni di linfa mesenterica e/o il trasporto di fluidi, cellule immunitarie, peptidi, proteine e molecole lipofile.

La linfa mesenterica Duct Cannulated Rat Modello: Applicazione alla valutazione di intestinale linfatico Drug Transport

Qui descriviamo un protocollo per eseguire cisterna magna inserimento di una canula (CMc), un modo minimamente invasivo per offrire elementi traccianti, substrati e molecole di segnalazione nel liquido cerebrospinale (CSF). In combinazione con differenti modalità di imaging, CMc permette glifatico sistema e valutazione dinamica di CSF, come pure la consegna di tutto il cervello di vari composti.

Impianto di cannula in Cisterna Magna di roditori

Qui descriviamo un metodo preciso per raccogliere linfociti del dotto toracico e osservare la migrazione dei linfociti intestinali-tropici nelle chiazze del ratto Peyer per 3 ore usando la fotografia time-lapse. Questa tecnica può chiarire come la dinamica dei linfociti sia influenzata in condizioni infiammatorie.

Osservazione microscopica della dinamica dei linfociti nelle patch di Rat Peyer

Qui, presentiamo un protocollo per definire chiaramente i passaggi per anastomizzare il linfonodo cervicale profondo o il suo vaso linfatico afferente con la vena facciale posteriore nel ratto, per ridurre al minimo l'influenza di altre variabili e standardizzare la conduzione della ricerca basata sulla chirurgia.

Protocolli chirurgici per l'anastomosi linfovenosa cervicale profonda in un modello di ratto: anastomosi linfonodali e vasi linfatici

Questo protocollo sperimentale descrive un modello di ratto canulato con canulazione dei dotti linfatici epatici che consente una valutazione diretta e quantitativa del drenaggio linfatico e vascolare epatico. Il modello consente il campionamento simultaneo di sangue linfatico epatico e sistemico per indagare il trasporto epatico, il metabolismo e la segnalazione immunitaria, supportando studi sulla farmacocinetica dei farmaci e sulla biologia specifica del fegato.

Un modello completo di ratti per la valutazione delle vie di trasporto epatico attraverso campionamenti simultanei di linfatica e vascolo sanguigna

Una tecnica per l'esecuzione di microscopia intravitale del linfonodo inguinale (LN) è delineato. Tale tecnica permette in tempo...

Microscopia intravitale del linfonodo inguinale

I recenti progressi nella microscopia a due fotoni hanno permesso in tempo...

Imaging intravitale del Nodo poplitea Lymph mouse

Impara la cannulazione del dotto linfatico cervicale profondo nel ratto per campionare direttamente la linfa cerebrale. Questo metodo consente di quantificare il trasporto linfatico e lo svuotamento di sostanze dal sistema nervoso centrale.

Modello di ratto canulato con dotto linfatico cervicale per la valutazione del trasporto linfatico dalla testa e dal cervello

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