Name: Mappatura dellimmunità infantile con input minimo: profilazione integrativa a cellula singola e multiomica (Video) | JoVE Journal
Uploaded: 2026-04-03T12:35:00.000+00:00
Duration: 10 min 29 s
Description: Scopri il profilo immunitario neonatale con tecniche multiomiche a cellula singola da campioni ematici minimi. Questo metodo consente studi longitudinali sullo sviluppo immunitario pediatrico.

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</ol>

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mappatura dell'immunità infantile con input minimo: profilazione integrativa a cellula singola e multiomica

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling

Mappatura dell'immunità infantile con input minimo: profilazione integrativa a cellula singola e multiomica

At birth, the neonatal immune system is abruptly confronted with a radically different environment, rich in microbial and environmental stimuli. ...

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JoVE Journal

Immunology and Infection

Immunologia e Infezioni

Questo protocollo descrive come valutare l'espressione di una vasta gamma di geni a livello clonale. Single-cell RT-qPCR produce risultati altamente affidabili con una forte sensibilità per le centinaia di campioni e geni.

Unicellulare espressione genica Utilizzando Multiplex RT-qPCR per caratterizzare eterogeneità delle rare popolazioni linfoidi

Qui descriviamo un approccio proteomico di singola cellula per valutare immunitario fenotipica e funzionale (induzione di citochine intracellulari) alterazioni nei campioni di sangue periferico intero, analizzati tramite citometria a massa.

Cella singola analisi di Immunophenotype e produzione di citochine nel sangue periferico intero tramite citometria a massa

Alla rinfusa gene espressione misure nube singola cella differenze nelle popolazioni eterogenee delle cellule. Qui, descriviamo un protocollo per analisi di espressione genica e l'indice come unicellulare ordinano di Florescence attivato Cell Sorting (FACS) possa essere combinato per delineare eterogeneità e immunophenotypically caratterizzare popolazioni molecolarmente distinte delle cellule.

Una combinatoria unicellulare approccio per la caratterizzazione molecolare e Immunophenotypic eterogeneità del tronco umano e popolazioni di cellule progenitrici

L'immunoprecipitazioni quantitativa multiplex (QMI) utilizza la citometria di flusso per rilevare sensitivemente le differenze nell'abbondanza di interazioni mirate proteina-proteina tra due campioni. QMI può essere eseguita utilizzando una piccola quantità di biomateriale, non richiede tag geneticamente ingegnerizzati e può essere adattato per qualsiasi rete di interazione proteica definita in precedenza.

Quantificazione delle dinamiche della rete di interazione proteica mediante co-immunoprecipitazioni multiplexate

L'uso di un approccio basato sull'RNA per determinare i profili immunitari quantitativi dei tessuti tumorali solidi e sfruttare le coorti cliniche per la scoperta di biomarcatori immuno-oncologici è descritto attraverso un protocollo molecolare e informatico.

Modellazione immunitaria predittiva dei tumori solidi

Qui, descriviamo una strategia semplice e accessibile per visualizzare, quantificare e mappare le cellule immunitarie nelle sezioni del tessuto tumorale incorporate con paraffina. Questa metodologia combina le tecniche di imaging e analisi digitale esistenti con lo scopo di espandere la capacità di multiplexing e l'analisi multiparametrica dei saggi di imaging.

Visualizzazione, quantificazione e mappatura delle popolazioni di cellule immunitarie nel microambiente tumorale

Nell'era dell'immunoterapia e della profilazione genomica a singola cellula, la biologia del cancro richiede nuovi strumenti in vitro e computazionali per studiare l'interfaccia tumore-immunità in un contesto spaziotemporale appropriato. Descriviamo protocolli per sfruttare co-colture microfluidiche tumore-immunitarie in contesti 2D e 3D, compatibili con il monitoraggio dinamico e multiparametrico delle funzioni cellulari.

Modelli di co-coltura microfluidica per la dissezione della risposta immunitaria in microambienti tumorali in vitro

Questo protocollo combina la citometria a flusso e il sequenziamento dell'RNA a singola cellula per isolare e caratterizzare gli stati delle cellule microgliali nel cervelletto dei cervelli di topo postnatale precoce. Utilizza la dissociazione enzimatica, la centrifugazione Percoll e l'immunocolorazione per rivelare l'eterogeneità delle microglia e migliorare la comprensione del loro ruolo nello sviluppo e nella malattia cerebellare.

Citometria a flusso e analisi di singole cellule per caratterizzare l'attivazione della microglia nello sviluppo precoce del cervello di topo postnatale

Una morte saggio basato su cellule di PTI in piante di Nicotiana benthamiana è descritto.

Test per patogeni associati modello molecolare (PAMP)-trigger Immunità (PTI) nelle piante

Selezione di Plasmodium falciparum Parassiti per Cytoadhesion alle cellule cerebrali umane endoteliali

Scopri il profilo immunitario neonatale con tecniche multiomiche a cellula singola da campioni ematici minimi. Questo metodo consente studi longitudinali sullo sviluppo immunitario pediatrico.

Mappatura dell'immunità infantile con input minimo: profilazione integrativa a cellula singola e multiomica

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