Name: Un modello di lesione da ipossia-reossigenazione in cardioidi umani autoassemblanti (Video) | JoVE Journal
Uploaded: 2026-03-17T12:35:00.000+00:00
Duration: 10 min 41 s
Description: Scopri come modellare lesioni da ipossia-reossigenazione in cardioidi umani autoassemblanti derivati da iPSC. Questo metodo consente di studiare risposte al danno cardiaco e testare interventi terapeutici in un contesto di tessuto umano.

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</ol>

<table><tbody><tr><td>Soluzione Accutase</td><td>Sigma-Aldrich</td><td>A6964-500ML</td><td></td></tr><tr><td>Anticorpo anti-cardiaco troponina T</td><td>Abcam</td><td>EPR20266</td><td></td></tr><tr><td>Integratore di B-27 (50X), senza siero</td><td>Gibco</td><td>17504044</td><td></td></tr><tr><td>Integratore B-27, senza insulina</td><td>Gibco</td><td>A1895602</td><td></td></tr><tr><td>Lettore di placche BioTek Synergy Neo2</td><td>BioTek</td><td></td><td></td></tr><tr><td>Cal-520, AM</td><td>AAT Bioquest</td><td>21131</td><td></td></tr><tr><td>CardioCount V1</td><td>Karra Lab</td><td>PMID: 38984052</td><td></td></tr><tr><td>Linea cellulare (Homo sapiens, maschio) - Linea iPSC DU11</td><td>Nucleo delle Cellule Staminali della Duke University</td><td></td><td></td></tr><tr><td>Lame di microscopio caricate, vetro bianco</td><td>VWR</td><td>89500-496</td><td></td></tr><tr><td>CHIR 99021</td><td>Tocris</td><td>4423</td><td></td></tr><tr><td>CloneR</td><td>Tecnologie STEMCELL</td><td>5888</td><td></td></tr><tr><td>Micropiastre di attacco Corning 96-Well Clear Ultra Low Attachment</td><td>Fisher</td><td>7007</td><td></td></tr><tr><td>Serbatoi di pipettolamento usa e getta</td><td>VWR</td><td>89094-678</td><td></td></tr><tr><td>DMEM/F-12, HEPES</td><td>Gibco</td><td>11330057</td><td></td></tr><tr><td>Microscopio fluorescente EVOS</td><td>EVOS</td><td></td><td></td></tr><tr><td>Matrice basale-membrana con fattore di crescita ridotto Geltrex, senza LDEV, qualificata per cellule staminali</td><td>Gibco</td><td>A1413302</td><td></td></tr><tr><td>Activina umana: una proteina ricombinante</td><td>PEPROTECH INC/Life Technologies</td><td>120-14E-50UG</td><td></td></tr><tr><td>IWP-2</td><td>Tecnologie STEMCELL</td><td>72124</td><td></td></tr><tr><td>L-Ascorbico Acido (Cristallino/ACS Certificato)</td><td>Fisher Scientific</td><td>A61-100</td><td></td></tr><tr><td>Anti-Vimentina per topi</td><td>DSHB</td><td>AMF-17b</td><td></td></tr><tr><td>mTeSR Plus</td><td>Tecnologie STEMCELL</td><td>100-0276</td><td></td></tr><tr><td>Piastre di coltura cellulare multipozzo &ndash; 12 well</td><td>VWR</td><td>10062-894</td><td></td></tr><tr><td>Pluronic F-127</td><td>Sigma-Aldrich</td><td>P2443</td><td></td></tr><tr><td>ReLeSR</td><td>Tecnologie STEMCELL</td><td>100-0483</td><td></td></tr><tr><td>RPMI 1640 Medium</td><td>Gibco</td><td>11875119</td><td></td></tr><tr><td>RPMI 1640 Medium, senza rosso fenolico</td><td>Gibco</td><td>11835-030</td><td></td></tr><tr><td>Thiazovivina</td><td>Tecnologie STEMCELL</td><td>72254</td><td></td></tr><tr><td>Composto Tissue-Tek O.C.T.</td><td>SAKURA FINETEK USA INC (VWR)</td><td>25608-930</td><td></td></tr><tr><td>Microscopio confocale a disco rotante Zeiss</td><td>Zeiss</td><td></td><td></td></tr></tbody></table>

un modello di lesione da ipossia-reossigenazione in cardioidi umani autoassemblanti

A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids

Un modello di lesione da ipossia-reossigenazione in cardioidi umani autoassemblanti

Without rapid revascularization, ischemic heart disease leads to irreversible loss of cardiomyocytes, scar formation, and decreased cardiac function. The ...

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JoVE Journal

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Medicina

Questo manoscritto descrive la creazione di tessuti cardiaci ingegnerizzati definiti utilizzando l'espressione di marcatori di superficie e la selezione cellulare. I tessuti definiti possono quindi essere utilizzati in un bioreattore multi-tessuto per studiare i meccanismi della terapia cellulare cardiaca al fine di fornire un sistema modello funzionale, ma controllato, del cuore umano.

Costruzione di Engineered Definito umani cardiache tessuti per studiare i meccanismi della terapia cellulare cardiaca

Qui, presentiamo un protocollo per descrivere un modello di recupero semplice bypass cardiopolmonare senza trasfusione o agenti inotropi in un ratto. Questo modello permette lo studio di lungo termine le conseguenze dell'esclusione cardiopolmonare multiple-organo.

Un modello di esclusione cardiopolmonare di recupero senza trasfusione o agenti inotropi in ratti

Presentiamo un modello di cardiopatia ischemica utilizzando cardiomiociti derivati da cellule staminali pluripotenti indotte dall'uomo, insieme a un metodo per la valutazione quantitativa dei danni ai tessuti causati dall'ischemia. Questo modello può fornire una piattaforma utile per lo screening farmacologico e ulteriori ricerche sulle cardiopatie ischemiche.

Modello di cardiomiociti ischemici e confronto video-based della contrazione cardiomiocite utilizzando cardiomiociti derivati da hiPSC

Gli effetti distinti dei diversi gradi di ipotermia sulla protezione del miocardio non sono stati valutati a fondo. L'obiettivo del presente studio era quantificare i livelli di morte cellulare a seguito di diversi trattamenti di ipotermia in un modello umano a base di cardiomiociti, gettando le basi per una futura ricerca molecolare approfondita.

Valutazione in vitro della protezione del miocardio a seguito dell'ipotermia-precondizione in un modello di miociti cardiaci umani

L'obiettivo di questo protocollo è spiegare e dimostrare lo sviluppo di un modello microfluidico tridimensionale (3D) di tessuto cardiaco umano altamente allineato, composto da cardiomiociti derivati da cellule staminali co-coltivati con fibroblasti cardiaci (FF) all'interno di un idrogel biomimetico a base di collagene, per applicazioni nell'ingegneria dei tessuti cardiaci, nello screening dei farmaci e nella modellazione delle malattie.

Sviluppo di tessuto cardiaco umano organizzato in 3D all'interno di una piattaforma microfluidico

La distanza spaziale è un parametro chiave nella valutazione del danno da ipossia/riossigenazione in un modello di co-coltura di strati cellulari endoteliali e cardiomiociti separati, suggerendo, per la prima volta, che l'ottimizzazione dell'ambiente spaziale di co-coltura è necessaria per fornire un modello in vitro favorevole per testare il ruolo delle cellule endoteliali nella protezione dei cardiomiociti.

Sviluppo di un modello di co-coltura cellulare per imitare l'ischemia/riperfusione cardiaca in vitro

Qui, descriviamo un protocollo per creare organoidi cardiaci umani rilevanti per lo sviluppo (hHO) in modo efficiente utilizzando cellule staminali pluripotenti umane mediante auto-organizzazione. Il protocollo si basa sull'attivazione sequenziale dei segnali di sviluppo e produce tessuti cardiaci umani altamente complessi e funzionalmente rilevanti.

Generazione di organoidi cardiaci umani auto-assemblanti derivati da cellule staminali pluripotenti

I tessuti cardiaci tridimensionali bioingegnerizzati utilizzando cardiomiociti derivati da cellule staminali sono emersi come modelli promettenti per lo studio in vitro del miocardio umano sano e malato, ricapitolando gli aspetti chiave della nicchia cardiaca nativa. Questo manoscritto descrive un protocollo per la fabbricazione e l'analisi di tessuti cardiaci ingegnerizzati ad alto contenuto generati da cardiomiociti derivati da cellule staminali pluripotenti indotte umane.

Progettazione di un bioreattore per migliorare l'acquisizione dei dati e modellare la produttività dei tessuti cardiaci ingegnerizzati

Qui descriviamo un protocollo in vitro per modellare danni da ipossia/reossigenazione nelle cellule epiteliali tubulari prossimali altamente metaboliche. Questo modello è progettato per indurre danni indotti dallo stress metabolico, che possono essere misurati attraverso l'espressione di marcatori di danno prossimale dei tubuli e la valutazione della funzione respiratoria mitocondriale.

Modellizzazione dell'ipossia/lesione da reossigenazione nelle cellule epiteliali tubolari prossimali

Un modello contusione di gravi lesioni del midollo spinale è descritto. Fasi di pre-operatoria, operatoria e post-operatoria dettagliate sono descritte di ottenere un modello coerente.

Un modello Contusione di gravi lesioni del midollo spinale nei ratti

Scopri come modellare lesioni da ipossia-reossigenazione in cardioidi umani autoassemblanti derivati da iPSC. Questo metodo consente di studiare risposte al danno cardiaco e testare interventi terapeutici in un contesto di tessuto umano.

Un modello di lesione da ipossia-reossigenazione in cardioidi umani autoassemblanti

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