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<ol>
	<li>Zeng H, Sanes JR. Neuronal cell-type classification: challenges, opportunities and the path forward. Nat Rev Neurosci. 2017;18(9):530-46.</li>
	<li>Macosko EZ, Basu A, Satija R, Nemesh J, Shekhar K, Goldman M, et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell. 2015;161(5):1202-14.</li>
	<li>Brand AH, Perrimon N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993;118(2):401-15.</li>
	<li>Coons AH, Kaplan MH. Localization of antigen in tissue cells: improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950;91(1):1-13.</li>
	<li>Pardue ML, Gall JG. Chromosomal localization of mouse satellite DNA. Science. 1970;168(3937):1356-8.</li>
	<li>Gall JG, Pardue ML. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A. 1969;63(2):378-85.</li>
	<li>Singer RH, Ward DC. Actin gene expression visualized in chicken muscle tissue culture by using in situ. hybridization with a biotinylated nucleotide analog. Proc Natl Acad Sci U S A. 1982;79(23):7331-5.</li>
	<li>Bauman JG, Wiegant J, Borst P, van Duijn P. A new method for fluorescence microscopical localization of specific DNA sequences by in situ. hybridization of fluorochrome-labelled RNA. Exp Cell Res. 1980;128(2):485-90.</li>
	<li>Herrington CS, Burns J, Graham AK, Evans M, McGee JO. Interphase cytogenetics using biotin- and digoxigenin-labelled probes. I. Relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells. J Clin Pathol. 1989;42(6):592-600.</li>
	<li>Tautz D, Pfeifle C. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila. embryos reveals translational control of the segmentation gene hunchback. Chromosoma. 1989;98(2):81-5.</li>
	<li>Rudkin GT, Stollar BD. High-resolution detection of DNA-RNA hybrids in situ. by indirect immunofluorescence. Nature. 1977;265(5593):472-3.</li>
	<li>van de Corput MP, Dirks RW, van Gijlswijk RP, van de Rijke FM, Raap AK. Fluorescence in situ. hybridization using horseradish peroxidase-labeled oligodeoxynucleotides and tyramide signal amplification for sensitive DNA and mRNA detection. Histochem Cell Biol. 1998;110(4):431-7.</li>
	<li>Schr&#246;der C, Tautz D, Seifert E, J&#228;ckle H. Differential regulation of the two transcripts from the Drosophila. gap segmentation gene hunchback. EMBO J. 1988;7(9):2881-7.</li>
	<li>Dirks RM, Pierce NA. Triggered amplification by hybridization chain reaction. Proc Natl Acad Sci U S A. 2004;101(43):15275-8.</li>
	<li>Choi HMT, Chang JY, Trinh LA, Padilla JE, Fraser SE, Pierce NA. Programmable in situ. amplification for multiplexed imaging of mRNA expression. Nat Biotechnol. 2010;28(11):1208-12.</li>
	<li>Femino AM, Fay FS, Fogarty K, Singer RH. Visualization of single RNA transcripts in situ. Science. 1998;280(5363):585-90.</li>
	<li>Trivedi V, Choi HMT, Fraser SE, Pierce NA. Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos. Development. 2018;145(1):dev156869.</li>
	<li>Choi HMT, Schwarzkopf M, Fornace ME, Acharya A, Artavanis G, Stegmaier J, et al. Third-generation in situ. hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust. Development. 2018;145(12):dev165753.</li>
	<li>Schulte SJ, Fornace ME, Hall JK, Shin GJ, Pierce NA. HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples. Development. 2024;151(4):dev202159.</li>
	<li>Schwarzkopf M, Liu MC, Schulte SJ, Ives R, Husain N, Choi HMT, et al. Hybridization chain reaction enables a unified approach to multiplexed, quantitative, high-resolution immunohistochemistry and in situ. hybridization. Development. 2021;148(22):dev199847.</li>
	<li>Ferreira AAG, Cordoba S, Rajesh R, Choi BJ, Desplan C. A developmental single-cell atlas of the Drosophila. visual system glia reveals cell type diversification and subcellular mRNA compartmentalization. bioRxiv. 2023. Available from: https://www.biorxiv.org/content/10.1101/2023.08.06.552169v2</li>
	<li>Herre M, Goldman OV, Lu TC, Caballero-Vidal G, Qi Y, Gilbert ZN, et al. Non-canonical odor coding in the mosquito. Cell. 2022;185(17):3104-23.e28.</li>
	<li>Olivares-Abril J, Joha J, Lee JY, Davis I. Optimization of hybridization chain reaction for imaging single RNA molecules in Drosophila. larvae. Fly (Austin). 2024;18(1):2409968.</li>
	<li>Burge S, Parkinson GN, Hazel P, Todd AK, Neidle S. Quadruplex DNA: sequence, topology and structure. Nucleic Acids Res. 2006;34(19):5402-15.</li>
	<li>Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403-10.</li>
	<li>Lovely AM, Duerr TJ, Stein DF, Mun ET, Monaghan JR. Hybridization chain reaction fluorescence in situ. hybridization (HCR-FISH) in Ambystoma mexicanum tissue. Methods Mol Biol. 2023;2562:109-22.</li>
	<li>Kuehn E, Clausen DS, Null RW, Metzger BM, Willis AD, Ozpolat BD. Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii. J Exp Zool B Mol Dev Evol. 2022;338(4):225-40.</li>
	<li>Hofbauer A, Campos-Ortega JA. Proliferation pattern and early differentiation of the optic lobes in Drosophila. melanogaster. Roux Arch Dev Biol. 1990;198(5):264-74.</li>
	<li>Li X, Erclik T, Bertet C, Chen Z, Voutev R, Venkatesh S, et al. Temporal patterning of Drosophila. medulla neuroblasts controls neural fates. Nature. 2013;498(7455):456-62.</li>
	<li>Hasegawa E, Kitada Y, Kaido M, Takayama R, Awasaki T, Tabata T, et al. Concentric zones, cell migration and neuronal circuits in the Drosophila. visual center. Development. 2011;138(5):983-93.</li>
	<li>Suzuki T, Kaido M, Takayama R, Sato M. A temporal mechanism that produces neuronal diversity in the Drosophila. visual center. Dev Biol. 2013;380(1):12-24.</li>
	<li>Zhu H, Zhao SD, Ray A, Zhang Y, Li X. A comprehensive temporal patterning gene network in Drosophila. medulla neuroblasts revealed by single-cell RNA sequencing. Nat Commun. 2022;13(1):1247.</li>
	<li>Konstantinides N, Holguera I, Rossi AM, Escobar A, Dudragne L, Chen YC, et al. A complete temporal transcription factor series in the fly visual system. Nature. 2022;604(7905):316-22.</li>
	<li>Konstantinides N, Kapuralin K, Fadil C, Barboza L, Satija R, Desplan C. Phenotypic convergence: distinct transcription factors regulate common terminal features. Cell. 2018;174(3):622-35.e13.</li>
	<li>Garcia-Alonso L, VanBerkum MF, Grenningloh G, Schuster C, Goodman CS. Fasciclin II controls proneural gene expression in Drosophila. Proc Natl Acad Sci U S A. 1995;92(23):10501-5.</li>
	<li>Simon F, Holguera I, Chen YC, Malin J, Valentino P, Njoo-Deplante C, et al. Spatial, temporal and Notch determination of terminal selector expression controls neuronal cell fate in the Drosophila. optic lobe. Nat Neurosci. 2026;29(5):1068-78.</li>
	<li>Malin JA, Chen YC, Simon F, Keefer E, Desplan C. Spatial patterning controls neuron numbers in the Drosophila. visual system. Dev Cell. 2024;59(9):1132-45.e6.</li>
	<li>Pinto-Teixeira F, Koo C, Rossi AM, Neriec N, Bertet C, Li X, et al. Development of concurrent retinotopic maps in the fly motion detection circuit. Cell. 2018;173(2):485-98.</li>
	<li>Erclik T, Li X, Courgeon M, Bertet C, Chen Z, Baumert R, et al. Integration of temporal and spatial patterning generates neural diversity. Nature. 2017;541(7637):365-70.</li>
	<li>&#214;zel MN, Simon F, Jafari S, Holguera I, Chen YC, Benhra N, et al. Neuronal diversity and convergence in a visual system developmental atlas. Nature. 2021;589(7840):88-95.</li>
	<li>Marques GS, Teles-Reis J, Konstantinides N, Brito PH, Homem CCF. Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila. PLoS Biol. 2023;21(5):e3002115.</li>
	<li>Pan Y, Rauskolb C, Irvine KD. Near-infrared nuclear markers for Drosophila. imaging. microPublication Biol. 2022;2022. Available from: https://www.micropublication.org/journals/biology/micropub-biology-000639</li>
</ol>

<table><tbody><tr><td>10% Tween-20</td><td>Bio-Rad Laboratories</td><td>1662404</td><td>NA</td></tr><tr><td>20x PBS, pH7.4</td><td>Growcells.com</td><td>MRGF-6396</td><td>2,74M NaCl, 53,7 mM KCl, 20,3 mM Na2HPO4, 29,4 mM KH2PO4&nbsp;</td></tr><tr><td>20x SSC</td><td>Quality Biological</td><td>351-003-131</td><td>3M NaCl, 0,3M Sodium Citrate</td></tr><tr><td>37 &deg;C incubator</td><td>Fisher Scientific (Isotemp)</td><td>15-015-2633</td><td>NA</td></tr><tr><td>37 &deg;C water bath</td><td>Fisher Scientific</td><td>FSGPD15D</td><td>NA</td></tr><tr><td>Aluminum foil</td><td>Reynolds Wrap/Fisher Brand</td><td>15-078-291</td><td>NA</td></tr><tr><td>Antibody: Mouse-anti-Abrupt</td><td>DSHB</td><td>Abrupt; RRID: AB_528344&nbsp;</td><td>Dilution: 1:20</td></tr><tr><td>Antibody: Chicken anti-GFP</td><td>Millipore Sigma</td><td>Catalog# 06-896; RRID: AB-619712</td><td>Dilution: 1:500</td></tr><tr><td>Antibody: Rabbit anti-GFP</td><td>Life Technologies</td><td>#11122; RRID: AB_221569</td><td>Dilution: 1:500</td></tr><tr><td>Antibody: Rabbit anti-Dll</td><td>Genscript/Desplan lab</td><td>-</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Rat anti-NCadherin</td><td>DSHB</td><td>DN-Ex #8, RRID: AB_528121</td><td>Dilution: 1:50</td></tr><tr><td>Antibody: Rabbit anti-SoxN</td><td>Genscript/Desplan lab</td><td>-</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Guinea Pig anti-Traffic jam</td><td>Gift from D. Godt</td><td>-</td><td>Dilution: 1:2.000</td></tr><tr><td>Antibody: Donkey anti-Rat 405</td><td>Jackson ImmunoResearch</td><td>Code # 712-475-150; RRID: AB_2340680</td><td>Dilution: 1:100</td></tr><tr><td>Antibody: Donkey anti-Chicken 488</td><td>Jackson ImmunoResearch</td><td>Code #703-545-155; RRID: AB_2340375</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Mouse 488</td><td>Jackson ImmunoResearch</td><td>Code #715-545-151; RRID: AB_2341099</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rabbit 488</td><td>Jackson ImmunoResearch</td><td>Code #711-545-152; RRID: AB_2313584</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rabbit A555</td><td>Thermo Scientific</td><td>Catalog #A-31572; RRID: AB_162543</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rat Cy3</td><td>Jackson ImmunoResearch</td><td>Code #712-165-153; RRID: AB_2340667</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Guinea Pig 647</td><td>Jackson ImmunoResearch</td><td>Code #706-605-148; RRID: AB_2340476</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rat 647</td><td>Jackson ImmunoResearch</td><td>Code #712-605-153; RRID: AB_2340694</td><td>Dilution: 1:200</td></tr><tr><td>Antifade mountant</td><td>Thermo Fisher Scientific</td><td>S36940 (SlowFade Gold Antifade Mountant)</td><td>Other slowfades will work as well, including ones with DAPI (S36936)</td></tr><tr><td>Confocal Microscope</td><td>Zeiss</td><td>LSM-880</td><td>NA</td></tr><tr><td>Cover slips (22 x 30)</td><td>VWR</td><td>16004-332</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: ab-GFP-FLAG</td><td>BDSC</td><td>38626; RRID: BDSC_38626</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: Canton S</td><td>BDSC</td><td>64349; RRID: BDSC_64349</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: dpp-Gal4</td><td>BDSC</td><td>1553; RRID: BDSC_1553</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: ham-GFP.FPTB</td><td>BDSC</td><td>83660; RRID: BDSC_83660</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: yw; 10xUAS-myr-GFP;</td><td>BDSC</td><td>32198; RRID: BDSC_32198</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: UAS-Stinger</td><td>BDSC</td><td>84277; RRID: BDSC_84277</td><td>NA</td></tr><tr><td>Fly: D. melanogaster:&nbsp; wit-KO-Gal4</td><td>BDSC</td><td>600136; RRID: BDSC_600136</td><td>NA</td></tr><tr><td>Forceps</td><td>Dumont</td><td>#5 or #55s</td><td>NA</td></tr><tr><td>Glass slides</td><td>VWR</td><td>16005-106</td><td>NA</td></tr><tr><td>Glycine</td><td>Millipore Sigma</td><td>G7126-10MG</td><td>NA</td></tr><tr><td>HCR Amplification buffer</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>NA</td></tr><tr><td>HCR-Gold Amplifiers</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>NA</td></tr><tr><td>HCR HiFi Probes</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>NA</td></tr><tr><td>HCR Probe Hybridization Buffer</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>Contains formamide</td></tr><tr><td>HCR Probe Wash Buffer</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>Contains formamide</td></tr><tr><td>Nail polish (clear)</td><td>Electron Microscopy Sciences</td><td>72180</td><td>NA</td></tr><tr><td>Nuclease-free water (not DEPC-treated)</td><td>Thermo Fisher Scientific</td><td>AM9937</td><td>NA</td></tr><tr><td>Nutating mixer</td><td>Corning/Fisher Scientific</td><td>10-320-100</td><td>NA</td></tr><tr><td>Paraformaldehyde, 16% (w/v)</td><td>Electron Microscopy Sciences</td><td>15710</td><td>Toxic</td></tr><tr><td>PCR Machine/Thermal Cycler</td><td>Bio-Rad</td><td>1851148 (C1000 Touch Thermal Cycler)</td><td>NA</td></tr><tr><td>PCR pull-apart tube strips with dome cap strips (0.2 mL)&nbsp;</td><td>USA Scientific</td><td>#1402-2400</td><td>NA</td></tr><tr><td>Petri dishes (150 x 15 mm; for making dissecting dishes)</td><td>Fisher Scientific</td><td>FB0875714</td><td>NA</td></tr><tr><td>RNase ZAP</td><td>ThermoFisher Scientific</td><td>AM9780</td><td>NA</td></tr><tr><td>Slide spacer stickers</td><td>SUNJin</td><td>IS016 (iSpacer stickers (0,2 mm deep))</td><td>NA</td></tr><tr><td>Sylgard elastomer kit (for making dissecting dishes)</td><td>Fisher Scientific</td><td>NC9285739</td><td>NA</td></tr><tr><td>Triton X-100</td><td>Merck Millipore Sigma</td><td>X100</td><td>NA</td></tr></tbody></table>

ibridazione a reazione a catena fish nel lobo ottico di drosophila in via di sviluppo

Hybridization Chain Reaction FISH in the Developing Drosophila Optic Lobe

Ibridazione a reazione a catena FISH nel lobo ottico di Drosophila in via di sviluppo

Understanding neural development requires precise characterization of when and where genes are expressed. Techniques such as in situ hybridization have ...

hybridization-chain-reaction-fish-developing-drosophila-optic

Research

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JoVE Journal

Neuroscience

Neuroscienze

Utilizzando il monte intero In situ Ibridazione a Link Biologia Molecolare e organismal

I neuroni sensoriali arborizzazione dendritica...

Analisi morfologica del Drosophila Larvale dendriti sensoriali e periferico Neuron assoni utilizzo Mosaici genetici

Qui si descrive un insieme di montaggio...

Tutta la Monte RNA fluorescente<em&gt; In situ</em&gt; L&#39;ibridazione di<em&gt; Drosophila</em&gt; Embrioni

Il pesce zebra, un piccolo pesce tropicale, è diventata un modello importante per studiare la funzione del gene durante lo sviluppo dei vertebrati e la malattia. L'espressione temporale e spaziale dei geni bersaglio può essere...

Alta Risoluzione intero monte<em&gt; In Situ</em&gt; Ibridazione in embrioni di zebrafish per studiare l&#39;espressione genica e la funzione

L'immunocolorazione è una tecnica efficace per visualizzare specifici tipi di cellule e proteine all'interno dei tessuti. Utilizzando la sonicazione, il protocollo qui descritto allevia la necessità di sezionare i tessuti di Drosophila melanogaster da embrioni e larve in fase avanzata prima dell'immunocolorazione. Forniamo una metodologia efficiente per l'immunocolorazione di larve intere di montatura fissate con formaldeide.

Sonicazione-facilitato immunofluorescenza colorazione di ritardo-fase embrionale e larvale<em&gt; Drosophila</em&gt; Tessuti<em&gt; In Situ</em

Descriviamo una procedura multi-target cromogenico tutto il montaggio di ibridazione in situ (MC-WISH) in embrioni di Drosophila intatte che permettono il rilevamento simultaneo e specifico di tre diversi modelli di distribuzione mRNA da contrastanti precipitati di colore.

Multi-bersaglio Cromogenico Whole-mount<em&gt; In Situ</em&gt; Ibridazione per il confronto Gene domini di espressione in<em&gt; Drosophila</em&gt; Embrioni

Il protocollo descritto di RNA in situ ibridazione del permette la rilevazione del RNA in embrioni della drosofila interi o tessuti dissecati. Utilizzando piastre microtiter a 96 pozzetti e amplificazione del segnale di microarray, trascrizioni possono essere rilevate alla velocità effettiva, sensibilità e alta risoluzione e ad un costo relativamente basso.

Ibridazione fluorescente In Situ ad alta risoluzione in embrioni della drosofila e tessuti mediante amplificazione del segnale Tyramide

Questo manoscritto presenta un metodo dettagliato per la generazione di X-cromosoma braccio sonde e performanti in situ ibridazione di fluorescenza (pesce) per esaminare lo stato della sorella coesione cromatidio in prometafase e metafase sono arrestato  Drosofila ovociti. Questo protocollo è adatto a determinare se la coesione meiotica braccio è intatto o perturbato in differenti genotipi.

Mediante ibridazione In Situ di fluorescenza (pesce) per monitorare lo stato di coesione braccio in prometafase e metafase I Oocytes di Drosophila

L'articolo descrive i metodi e i reagenti necessari per eseguire la reazione a catena di ibridazione RNA a fluorescenza intera in situ (HCR, RNA, WM-FISH) per rivelare informazioni sulla risoluzione spaziale e cellulare dei geni dei recettori chemiosensoriali nell'antenna della zanzara e nel palpo mascellare.

Reazione a catena di ibridazione RNA Fluorescenza a montaggio intero Ibridazione in situ di geni chemiosensoriali nelle appendici olfattive delle zanzare

L'ibridazione in situ a fluorescenza iterativa assistita da espansione (EASI-FISH) è una tecnica robusta che combina la microscopia ad espansione con l'ibridazione in situ a fluorescenza (FISH) per rilevare l'espressione di più geni nei tessuti spessi. Questo protocollo delinea i recenti progressi nel metodo e il suo adattamento per l'etichettatura del sistema nervoso centrale della Drosophila intatta.

Rilevamento multiplex dell'espressione genica nel cervello intatto di Drosophila mediante ibridazione in situ a fluorescenza iterativa assistita da espansione

New York University.  Questo protocollo descrive i passaggi necessari per eseguire ibridazione ibridazione a reazione a catena fluorescente in situ (HCR-FISH) nei lobi ottici larvali di Drosophila melanogaster . Sono dettagliate procedure per la progettazione della sonda, l'ibridazione, l'amplificazione e il montaggio dei tessuti per facilitare la visualizzazione del lobo ottico in sviluppo.

Ibridazione a reazione a catena FISH nel lobo ottico di Drosophila in via di sviluppo

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