Name: how cells control product buildup
Uploaded: 2019-03-11T09:21:10.000Z
Duration: 46 s
Channel: JoVE

フィードバック阻害と 呼ばれる調節過程で 反応の生成物はそれを生産した 代謝経路の 早い段階で酵素を阻害して 自らの生産を遅らせるか 停止させます 生成物は 基質に結合する活性部位とは異なる 酵素の調節部位に結合して これを行います 生成物が調節部位に結合すると 酵素に立体配座変化が生じ 引き続き反応に 触媒作用を及すのを妨げます この活動で 過度に高レベルの生成物が 蓄積するのを防ぎます レベルが十分に下がると 生成物は 酵素を阻害しなくなり 通常通り生成されていきます

Sander, Timur, Niklas Farke, Christoph Diehl, Michelle Kuntz, Timo Glatter, and Hannes Link. "Allosteric Feedback Inhibition Enables Robust Amino Acid Biosynthesis in E. Coli by Enforcing Enzyme Overabune." Cell Systems 8, no. 1 (2019年1月23日): 66-75.e8.[<a href="https://doi.org/10.1016/j.cels.2018.12.005" target="__blank">ソース</a>]

「ホモクエン酸合成酵素のL-リジンフィードバック阻害の構造的基礎」 The Journal of Biological Chemistry 285, no. 14 (2010年4月2日): 10446-53.[<a href="https://doi.org/10.1074/jbc.M109.094383" target="__blank">ソース</a>]

フィードバック阻害

Feedback Inhibition

細胞内では絶えず生化学反応が起こっており、出発物質をさまざまな反応物へ変換していますが、たいていは反応を促進する酵素の助けを借りています。酵素がなければ、ほとんどの反応が起こるのに長時間を要し、細胞が利用することができません。
 
 酵素は反応速度をコントロールする手助けを行うので、出発物質や、中間代謝物、生成物の適切な量が細胞内で維持されるように、酵素活性は制御されます。過剰な物質生成や枯渇は、細胞や生体の健康に悲惨な結果をもたらしうるのです。
 
 フィードバック阻害と呼ばれる制御プロセスでは、反応の生成物が、それを生成した代謝経路の初期段階にある酵素を阻害します。生成物は、基質と結合する活性部位とは異なる、酵素の調節部位に結合することで、自身と同じ生成物の生成を遅らせたり、停止させます。生成物が調節部位に結合すると、酵素の構造が変化し、基質との結合が阻止されて、その触媒反応が継続しないようにします。
 
 これにより、細胞内で高濃度の生成物が過剰に蓄積されるのを防ぐことができます。生成物の量が十分に減ると、生成物による酵素の阻害はなくなり、通常通りの反応が進むようになります。

生化学反応は細胞内で絶えず起こっており、通常は酵素の助けを借りて、出発物質をさまざまな製品に変換します...

Education

JoVE Core

Biology

生物学

Cellular Processes

細胞プロセス

Metabolism

代謝

KEY TERMS AND CONCEPTS

主要用語と概念

how cells control product buildup

Cells use enzymes to run biochemical reactions and make the products they need. These reactions are happening constantly, and enzymes speed them up so they happen fast enough to matter. Enzyme activity also has to be regulated. Cells need the right amounts of starting materials, intermediate metabolites, and final products.

If these substances build up too much or drop too low, the cell can be harmed. That is why cells use control systems to keep metabolic levels balanced. One important control process is feedback inhibition. In this process, the final product of a reaction slows an enzyme from an earlier step in the same pathway.

The product binds to a regulatory site on the enzyme. This site is different from the active site, which is where the substrate normally binds. Binding at the regulatory site changes the enzyme’s shape. That conformational change prevents the enzyme from binding the substrate and keeps the reaction from continuing.

Feedback inhibition helps stop too much product from accumulating in the cell. When product levels fall, the product no longer inhibits the enzyme. The pathway can then start again and continue producing the molecule as needed.

How Cells Control Product Buildup

what is metabolism?

Metabolism, Energy, and Cell Reactions

Metabolism is the set of chemical reactions that happen in a cell. It includes anabolism, which builds molecules, and catabolism, which breaks them down. These reactions help cells manage energy and keep life processes running. Cells use metabolism to turn food into energy, make cell parts, and remove waste products. Food stores potential energy in the bonds between atoms. Catabolic pathways break large food molecules into smaller ones and release that energy. Anabolic pathways use that energy...

first law of thermodynamics

Energy Flow in a Food Web

Energy flow in a food web shows the First Law of Thermodynamics in action. Energy cannot be created or destroyed. It can only change form as it moves through living things and the environment. Sunlight is the starting point. Plants capture radiant energy from the sun during photosynthesis and turn it into chemical energy stored in complex carbohydrates. At the same time, oxygen is released into the atmosphere. Herbivores eat the plants and use those sugars during digestion. The energy is...

second law of thermodynamics

Entropy in Food Web Energy Flow

The Second Law of Thermodynamics explains why entropy increases as energy moves through a system. Entropy means the amount of disorder in a system. Each time energy is transferred or transformed, some energy is lost as unusable heat. That heat can raise the motion of nearby molecules for a short time and make the surroundings less ordered and more random. This idea also appears in a food web. Primary producers, such as plants, take in energy from the sun and make food. During this process,...

kinetic energy

Motion as a Source of Energy

Kinetic energy is the energy of motion. It appears when an object is moving and can do work or cause change. A waterfall is one clear example. Water behind a dam has potential energy, but when it rushes downward, that moving water has kinetic energy. When the water reaches the bottom and stops moving, its energy can become potential energy again. Kinetic energy also shows up in living systems. Plants absorb light particles, called photons, which move very fast and help create chemical energy...

potential energy

Stored Energy in Motion and Bonds

Stored energy in motion and bonds is called potential energy. It depends on the structure of an object or the position of matter in space. Raising a kettlebell changes its location and increases its potential energy. A stretched rubber band also stores potential energy, which can later change into kinetic energy. Chemical bonds between atoms hold another form of stored energy called chemical energy. These bonds involve attractive forces, and that energy can change into other forms during a...

free energy

Gibbs Free Energy in Chemical Reactions

Gibbs free energy is a measure of useful energy that can be taken from a chemical reaction to do work. It is written as G and named for scientist Gibbs, who discovered it. This energy is what remains after entropy is considered. A reaction can either take in energy or release it. Reactions that absorb energy are called endergonic reactions. Reactions that release energy are called exergonic reactions. The direction of energy flow helps show which type of reaction is happening. Plants carry...

activation energy

How Catalysts Lower Reaction Barriers

Activation energy is the minimum energy a chemical reaction needs to keep going. When the activation energy is higher, the reaction usually happens more slowly. Adding heat can speed the reaction because molecules move faster and collide more often. As reactants collide, bonds break and the reaction moves through an uphill phase. That step creates the transition state, which is an unstable, high-energy state of the reactants. After that, new bonds form, products appear, and free energy is...

hydrolysis of atp

ATP Breakdown and Energy Release

ATP breakdown releases energy for cell work. Adenosine triphosphate, or ATP, can lose one or two phosphate groups when water is added. This hydrolysis reaction is exergonic, which means it releases energy that the cell can use. When one phosphate group is removed, ATP becomes ADP, or adenosine diphosphate. Inorganic phosphate is also released. ADP can then be hydrolyzed again to form AMP, or adenosine monophosphate, after a second phosphate group is removed. ATP has a clear structure. It is...

phosphorylation

Phosphorylation and Protein Control

Phosphorylation helps control how proteins work in cells. It is the addition or removal of phosphate groups, and it is the most common chemical change that regulates cellular processes. These changes can alter a protein’s structure, activity, stability, location inside the cell, and interactions with other proteins. During phosphorylation, protein kinases move the terminal phosphate group from ATP to a specific amino acid side chain on a target protein. The amino acids most often...

induced-fit model

How Enzymes Adjust to Their Substrates

Enzymes speed up many chemical reactions in cells. They are biological catalysts, so they increase reaction speed without being consumed or permanently changed. They do this by lowering the activation energy needed to turn reactants into products. Enzymes are proteins that usually work by binding to a substrate, which is the reactant molecule they act on. They show substrate specificity, meaning they bind only certain substrates. This specificity depends mainly on the shape and chemical...

enzyme kinetics

Substrate Effects on Enzyme Rates

Enzyme kinetics examines how enzymes change the rate of a reaction. Enzymes speed reactions by lowering the activation energy of the reactants. The reaction rate is the speed at which an enzyme turns reactants into products, and it can be affected by several factors, including the number of available reactants. Scientists often study enzyme kinetics in a test tube with a fixed amount of enzyme. When more substrate, or reactant, is added, the reaction rate increases because the enzyme can make...

enzyme inhibition

Competitive vs Noncompetitive Inhibitors

Enzyme inhibitors are molecules that reduce enzyme activity by binding to enzymes. Cells use these inhibitors to help regulate normal enzyme function. Drugs and toxins can also block enzymes and change how they work. Some inhibitors bind at the active site, which is the part of the enzyme where the substrate normally fits. Competitive inhibitors do this by occupying the active site and preventing the substrate from binding. If the substrate concentration becomes high enough, it can outcompete...

feedback inhibition

Cells use enzymes to run biochemical reactions and make the products they need. These reactions are happening constantly, and enzymes speed them up so they happen fast enough to matter. Enzyme activity also has to be regulated. Cells need the right amounts of starting materials, intermediate metabolites, and final products. If these substances build up too much or drop too low, the cell can be harmed. That is why cells use control systems to keep metabolic levels balanced. One important...

allosteric regulation

Allosteric Control of Enzyme Speed

Allosteric control changes enzyme speed when a molecule binds to a site other than the active site. That alternate binding spot is called an allosteric site. An enzyme can have more than one allosteric site. This type of control can raise or lower enzyme activity. Many enzymes with allosteric control are metabolic enzymes. They help with the breakdown or the building of specific molecules in cells. In allosteric inhibition, the effector molecule causes a shape change that lowers the enzyme's...

cofactors and coenzymes

Enzyme Helpers: Metals, Vitamins, and More

Enzyme helpers are needed for many enzymes to work properly. These helpers fall into two groups: cofactors and coenzymes. Cofactors are metallic ions, while coenzymes are non-protein organic molecules. Both can be tightly bound to an enzyme or attach only when the substrate binds. Cofactors are common in mature proteins, appearing in about 30% of them. They are often added as the enzyme folds and help with catalytic activity. Magnesium is one important cofactor, and it is needed by more than...

key terms and concepts

Learn how feedback inhibition lets cells regulate enzymes, stop product buildup, and keep metabolic reactions balanced in high school biology.

How Cells Control Product Buildup | JoVE K12

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How Cells Control Product Buildup

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