Name: nuclear dna, chromatin, and nucleoli
Uploaded: 2023-04-30T10:29:41.000Z
Duration: 1 min 25 s
Channel: JoVE

K12 Standards

north-carolina-k-12-science-standard-course-of-study

NC K-12 Science Standard Course of Study

9-12

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms- Structures and Processes

LS.Bio.1.5

The Nucleus

真核細胞では、核は細胞の遺伝情報であるDNAを収容する膜結合オルガネラです。
ただし、核内のDNAの組織は細胞周期の段階に依存します。
例えば、細胞が分裂していないとき、クロマチン繊維として知られるDNAタンパク質複合体は核質内に緩く浮遊しているため、DNA複製酵素や転写酵素に簡単にアクセスできます。
一方、細胞が分裂しようとしているとき、クロマチン繊維は染色体に圧縮されます。
核小体は、リボソーム(タンパク質の翻訳に関与するRNA-タンパク質複合体)の組み立てに特化した核内の明確な領域です。
核は二重膜バリア、つまり核膜に囲まれており、核孔複合体またはNPCが並んでいます。
これらのNPCは、核と細胞質との間の分子交換を制御します。
体内のほとんどの細胞は1つの核を持っていますが、赤血球のように核が開いていない、つまり核がない細胞もあります。一方、骨格筋細胞などの他の細胞は、構造的および機能的なニーズのために多核化されています。

核は、真核細胞の調節センターとして機能する膜に囲まれたオルガネラです。 これには、遺伝子発現を調節し、細胞内のタンパク質の生成を正確に調節する染色体 DNA が含まれています。 対照的に、ミトコンドリアと葉緑体の内部の DNA は、それらのオルガネラに特有の機能のみを実行します。

核内での DNA の配置

核内の遺伝子発現の調節は、DNA 構造を含む多くの要因に依存します。 核内の DNA はヒストンと呼ばれるタンパク質に包まれており、クロマチンと呼ばれる DNA-タンパク質複合体を形成しています。 細胞が分裂していないとき、つまり間期では、クロマチンは拡散的に組織化されます。 これにより、転写中のDNAへのアクセスが容易になります。 真核細胞が分裂しようとしているとき、クロマチンはしっかりと凝縮して別個の直線状の染色体になり、これは娘細胞に簡単に分離できます。

核小体

核小体は、核内のリボソーム RNA の染色体座の周囲に形成される膜のないオルガネラです。 したがって、核小体は rRNA の合成とリボソームサブユニットの組み立ての部位です。 これらのサブユニットは後に核の外に搬出され、完全に機能するリボソームに組み立てられます。

追加の核内構造

核には、カハール体、スペックル、パラスペックルなど、他の多くの核小体も含まれています。 これらの核小体は、核環境を空間的に区画化し、特定の生物学的反応のための異なる部位を作り出すのに役立ちます。 さらに、膜のない構造により、核質との内容物の交換が容易になります。

核は、真核細胞の制御センターとして機能する膜結合オルガネラです。それは遺伝子発現を制御する染色体DNAを含んでいます...

Education

JoVE Core

Cell Biology

細胞生物学

Unit 1

ユニット1

Cells, Genomes, and Evolution

細胞、ゲノム、そして進化

KEY TERMS AND CONCEPTS

主要用語と概念

nuclear dna, chromatin, and nucleoli

The nucleus is a membrane-bound organelle that stores chromosomal DNA and helps control what proteins a eukaryotic cell makes. It acts as the cell’s control center. DNA in mitochondria and chloroplasts is different because it mainly supports functions specific to those organelles.

Inside the nucleus, DNA is wrapped around proteins called histones. This DNA-protein complex is called chromatin. During interphase, when the cell is not dividing, chromatin stays loosely arranged so the DNA is easy to access for transcription. As a eukaryotic cell prepares to divide, the chromatin condenses into distinct, linear chromosomes that can be separated into daughter cells.

The nucleus also contains the nucleolus, which is a membrane-less organelle. It forms around chromosomal regions that contain ribosomal RNA, or rRNA. The nucleolus is the site of rRNA synthesis and the assembly of ribosomal subunits. These subunits later leave the nucleus and join together to form functional ribosomes.

Other nuclear bodies are present as well. Cajal bodies, speckles, and paraspeckles help organize the nuclear space into regions for specific biological reactions. Their membrane-less structure also allows materials to move more easily with the nucleoplasm, the fluid inside the nucleus.

Nuclear DNA, Chromatin, and Nucleoli

what are cells?

Cells as the Building Blocks of Life

Cells are the basic building blocks of life. A bacterium can be a single cell, while a human is made of trillions of cells. Even with this range, cells share key features that support life. A living cell has a plasma membrane. This is a bilayer of lipids that separates the cytoplasm inside the cell from the outside environment. A cell also carries genetic information in the form of DNA. In prokaryotic cells, DNA is found in a region called the nucleoid. In eukaryotic cells, DNA is enclosed...

the tree of life - bacteria, archaea, and eukaryotes

the-tree-of-life-bacteria-archaea-and-eukaryotes

Three Domains on the Tree of Life

The tree of life shows how living things are grouped into three domains: Bacteria, Archaea, and Eukaryotes. This classification helps explain the major branches of life and how they are related. Bacteria and Archaea are both single-celled organisms, but they are not the same. Eukaryotes include organisms with more complex cells, such as plants, animals, and fungi. Together, these three domains give a broad picture of life on Earth. Understanding the tree of life helps students compare cell...

prokaryotic cells

How Prokaryotes Survive Without a Nucleus

Prokaryotes are small unicellular organisms that live without a nucleus. They include the domains Archaea and Bacteria. Bacteria include common microbes such as Salmonella and E. coli, while Archaea include extremophiles that live in harsh places like volcanic springs. Prokaryotic cells still share several basic features with eukaryotic cells. They have a plasma membrane, cytoplasm, ribosomes, and genetic material in the form of single, circular DNA. Their cell parts float freely in the...

eukaryotic compartmentalization

How Organelles Keep Cells Working

Eukaryotic cells keep their organelles separate so each part can do a specific job. Organelles such as the nucleus and mitochondria are surrounded by membranes. These membranes are selectively permeable, which means they control what enters and leaves. That control creates small internal environments with conditions suited to each organelle. These controlled spaces help organelles work more efficiently. In animal cells, lysosomes keep an acidic environment that helps their enzymes digest...

eukaryotic evolution

How Eukaryotic Cells Became Complex

Eukaryotic cells became complex through a debated evolutionary path that likely involved endosymbiosis. The most accepted model is the endosymbiont theory, which explains how organelles such as mitochondria and chloroplasts may have entered an ancestral cell. Other ideas include the nucleus-first hypothesis, the mitochondria-first hypothesis, and the eukaryote-first hypothesis. The nucleus-first hypothesis says an ancestral prokaryote first formed a membrane around its DNA, creating the...

animal and plant cell structure

Plant vs Animal Cell Division and Specialization

Plant and animal cells differ in how they divide, organize into tissues, and specialize for different jobs. Both cell types divide by mitosis in non-gametic cells and by meiosis in gametic cells, but the details are not the same. These differences help explain how each cell type supports growth and reproduction. During cell division, animal cells use centrosomes to organize spindle fibers and separate chromosomes. Plant cells do not have centrosomes. Instead, they use a microtubule-organizing...

cytoplasm

Cytoplasm and the Cell’s Internal Support

The cytoplasm is the cell’s internal space that holds organelles and the cytoskeleton. It is suspended in the cytosol, an aqueous fluid made of water, ions, salts, and many organic molecules. This fluid helps keep the cell organized and supports many cellular activities. The cytoplasm is also where protein synthesis and protein folding take place. The watery cytosol helps proteins fold correctly. In a folded protein, hydrophobic amino acid side chains tend to stay buried in the core, while...

the nucleus

The nucleus is a membrane-bound organelle that stores chromosomal DNA and helps control what proteins a eukaryotic cell makes. It acts as the cell’s control center. DNA in mitochondria and chloroplasts is different because it mainly supports functions specific to those organelles. Inside the nucleus, DNA is wrapped around proteins called histones. This DNA-protein complex is called chromatin. During interphase, when the cell is not dividing, chromatin stays loosely arranged so the DNA is easy...

the dna helix

How DNA Structure Was Discovered

DNA is the genetic material that passes traits from one generation to the next in organisms and most viruses. It is made of two strands of nucleotides that twist into a spring-like double helix. The shape is not perfectly even. It also has repeating grooves called the major groove and the minor groove. The major groove forms where the sugar-phosphate backbones are farther apart. This wider space gives DNA-binding proteins, including transcription factors, better access to the bases. The minor...

the central dogma

DNA to Protein: RNA’s Role

DNA to protein flow depends on RNA as the link between genetic information and the amino acids that build proteins. Early scientists knew that DNA stores the information for cell functions and that proteins carry out most of those functions. For a long time, they did not know how the code in DNA became a working protein. At first, many people thought one gene was changed directly into its protein. Two findings in eukaryotic cells challenged that idea. Protein production does not happen in the...

mutations

Mutations in DNA and Inherited Disease

Mutations are changes in the DNA sequence. They can happen on their own during DNA copying, or they can be caused by environmental factors. Scientists describe mutations in several ways, including whether they change the amino acid sequence of a protein, how much DNA they affect, and whether they occur in somatic cells or germline cells. Some mutations are point mutations. A point mutation changes a single nucleotide in DNA. Other mutations are chromosomal alterations, which change the number...

genome size and the evolution of new genes

genome-size-and-the-evolution-of-new-genes

Genome Size and Gene Evolution

Genome size and gene evolution are linked through changes in DNA sequence and genome structure. Some genes can arise from parts of the genome that were not coding before, while others come from changes to existing genes. These shifts help explain how new genetic material can appear over time. A genome is the full set of DNA in an organism. Its size can change as DNA is added, removed, or rearranged. The transcript highlights that genome size is not fixed, and that these changes can shape the...

gene families

Shared Origins of Gene Families

Gene families are groups of related genes that share a common origin. They form when genes are copied and then change over time. These related genes can be found within the same genome. The video also connects gene families to homologous genes and orthologous genes. Homologous genes are genes with a shared evolutionary origin. Orthologous genes are homologous genes found in different species. Gene families help show how these relationships are used to study evolution and related gene functions.

gene evolution - fast or slow?

Gene Evolution Rates and Selection

Gene evolution can happen at different rates, and natural selection helps shape those changes. Some genes change slowly because important DNA changes are removed over time. Other genes can change more quickly when different mutations are kept in a population. The rate of evolution depends on how a gene is used and how strongly it is affected by selection. Genes with essential jobs often stay more similar across species. Genes under weaker pressure may show more variation and may evolve faster.<br...

types of genetic transfer between organisms

types-of-genetic-transfer-between-organisms

Vertical and Horizontal Gene Transfer

Genetic transfer between organisms happens in two main ways: vertical gene transfer and horizontal gene transfer. These two routes explain how hereditary material moves from one generation to the next or between organisms that are not parent and offspring. Vertical gene transfer passes genes from parent to offspring during reproduction. This is the usual way genetic information is inherited in living things. Horizontal gene transfer moves genetic material between organisms outside of...

key terms and concepts

Explore how nuclear DNA is packed as chromatin, condenses into chromosomes, and how the nucleolus makes rRNA and ribosomal subunits in eukaryotic cells.

Nuclear DNA, Chromatin, and Nucleoli | JoVE K12

cell biology

JoVE Core 細胞生物学は、複雑な細胞プロセスを、わかりやすく包括的なアニメーション ビデオ レッスンで説明します。このコレクションは、細胞小器官や膜、細胞輸送、細胞のエネルギー学など、細胞の必須コンポーネントと機能に焦点を当てています。これは、実際の研究実験における細胞生物学の応用を実証する科学者による実際のビデオによって補足されています。

Louisiana Student Standards for Science

High School

Life Sciences

heredity-inheritance-and-variation-of-traits

Heredity: Inheritance And Variation Of Traits

HS-LS3-1

Virginia  Standards of Learning

HIgh School

BIO.2

BIO.2.d

south-carolina-college-and-career-ready-science-standards

South Carolina College and Career Ready Science Standards

From Molecules to Organisms: Structures and Processes

B-LS1-1.

Compounds Essential to Human Function

The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all ...

Macromolecules

Biomolecules
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Solution Preparation
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Identifying Macromolecules with Indicator Solutions
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pH Regulation in Cells

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Chemistry of the Cell

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Introduction to Carbohydrates

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Carbohydrate Metabolism

Sugars as Energy Storage Molecules

What are Lipids?

lipid-derived-compounds-in-the-human-body

Lipid-derived Compounds in the Human Body

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Fats as Energy Storage Molecules

What are Proteins?

Protein Organization

Globular Proteins

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Fibrous Proteins

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Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. ...

Role of Proteins in the Human Body

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Nucleic Acids and Nucleotides

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell&#39;s genetic blueprint and have instructions for its ...

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group ...

ATP Energy Storage and Release

ATP and Macromolecule Synthesis

Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, ...

Construct an explanation to illustrate relationships between structure and function of major macromolecules essential for life.

LS.Bio.1.1

Introduction to Enzymes

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Enzymes and Activation Energy

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Introduction to Enzyme Kinetics

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Turnover Number and Catalytic Efficiency

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Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at ...

introduction-to-mechanisms-of-enzyme-catalysis

Introduction to Mechanisms of Enzyme Catalysis

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Ligand Binding and Linkage

Cooperative Allosteric Transitions

Ribozymes

Carry out investigations to illustrate how enzymes act as catalysts for biochemical reactions and how environmental factors affect enzyme activity.

LS.Bio.1.2

What are Cells?

Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium or trillions of them, ...

Cell Size

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Eukaryotic Compartmentalization

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Prokaryotic Cells

Prokaryotes are small unicellular organisms that include the domains&#x2014;Archaea and Bacteria. Bacteria include many common organisms, such as ...

Cytoplasm

The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The ...

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression ...

Endoplasmic Reticulum

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Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that ...

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are ...

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules ...

Peroxisomes

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration&#x2014;the ...

Introduction to the Cytoskeleton

Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ...

Introduction to Actin

Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle ...

Cell Structure

Background
Cells represent the most basic biological units of all organisms, whether it be simple, single-celled organisms like bacteria, or large, ...

overview-of-myosin-structure-and-function

Overview of Myosin Structure and Function

Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with ...

Visualizing Onion and Cheek Cells
ExpandImmediately before the experiment, wash and peel onion bulbs for the class.
Remove the entire brown outer skin and ...

Microtubules

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The Structure of Intermediate Filaments

The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of ...

Types of Intermediate Filaments

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Gap Junctions

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The Extracellular Matrix

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Tissues

Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main ...

Plant Cell Wall

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Plasmodesmata

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Use models to explain how the structure of organelles determines its function and supports overall cell processes.

LS.Bio.1.3

Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium, or trillions of them, ...

The Tree of Life - Bacteria, Archaea, and Eukaryotes

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Prokaryotic vs. Eukaryotic Cells

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Construct explanations to compare prokaryotic and eukaryotic cells in terms of structures and degree of complexity.

LS.Bio.1.4

Analyze how the relationship between structure and function supports life processes within organisms.

Nuclear DNA, Chromatin, and Nucleoli

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