循環器系の生理学

Before the blood pressure exercise, inspect all sphygmomanometer components including the tubing, cuff, manometer, and bulb for damage. Prepare alcohol swabs and stethoscopes at the front of the classroom. Ensure all equipment is undamaged and functional before students begin measurements to guarantee accurate results and safe handling.

Float the plastic bag containing fish in the 10-gallon tank for approximately 20 minutes to allow water temperatures to equalize. Then carefully open the bag, add some tank water, and gently pour the fish into the tank. Do not feed fish on their arrival day; begin feeding the next day with just a pinch of crushed food.

Set up two 10-gallon tanks near a window and fill with 9 gallons of water. Add dechlorinator according to bottle directions and liquid beneficial bacteria per manufacturer instructions. Install and run the filter for one week before adding fish. This cycling period establishes beneficial bacteria, creating a healthy environment for the animals.

Change 10 percent of the tank water daily, ensuring fresh water matches the tank temperature. Add fresh dechlorinator with each water change. Monitor water quality and temperature continuously. These practices maintain stable conditions necessary for accurate fish respiration measurements throughout the lab.

Set up five water baths labeled with different temperature conditions one hour before class. Fill each halfway with aquarium water or dechlorinated tap water and adjust temperatures accordingly. Use dechlorinated ice cubes to cool water below 20-22°C if needed. Place a temperature probe in each bath to monitor accuracy.

The one-week cycling period allows beneficial bacteria to establish in the tank, creating a healthy aquatic environment. This biological foundation removes harmful ammonia and nitrites produced by fish waste, stabilizing water chemistry. Without proper cycling, fish experience stress and poor water quality, compromising the reliability of respiration measurements.

Prepare enough clear containers with screw caps for each pair of students to have two. Set up five water baths with temperature probes at the front of the classroom. Ensure all tanks are cycling properly and fish are acclimated before class begins. These materials enable students to measure fish respiration across different temperature conditions using the scientific method steps and applications procedure.