4.6:

4.6: アロステリックレギュレーション

Allosteric Regulation

JoVE Core
Molecular Biology

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JoVE Core Molecular Biology

Allosteric Regulation

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4.5: Cofactors and Coenzymes

4.7: Ligand Binding and Linkage

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01:08 min

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November 23, 2020

Overview

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or synthesis of specific cellular molecules.

Allosteric Inhibition

In allosteric inhibition, the binding of an effector molecule to the allosteric site causes a conformational change that reduces the affinity of the enzyme for the substrate. Frequently, the allosteric inhibitor is a product of the enzyme or the enzyme pathway, allowing enzymatic products to limit their own production. This type of feedback inhibition prevents overproduction of products. As a classic example, isoleucine is an allosteric inhibitor of an enzyme important in its own synthesis.

Allosteric Activation

In contrast, an allosteric activator causes a conformational change that increases the affinity of the enzyme for its substrate. Allosteric activation dramatically increases the rate of reaction, as represented by the hyperbolic curve on a reaction rate versus substrate concentration graph. As an example, extracellular ligand binding to the transmembrane EGF receptor causes a conformational change that results in an increase in the intracellular kinase activity of the receptor. If an enzyme is composed of multiple subunits, binding of an allosteric activator to a single subunit can cause an increase in affinity and shape change for all of the affiliated subunits.

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Transcript

Allosteric regulation is the control of an enzyme's activity through sites distinct from its active site.

When an effector molecule binds to such an allosteric site, it can induce a conformational change in the enzyme.

This change may increase the affinity of the enzyme's active sites for its substrates, enhancing the enzyme's activity— a process known as allosteric activation.

The reaction rates for many allosteric enzymes create a positive S-shaped curve when graphed against increasing substrate concentrations.

When a positive effector is added, the allosteric activation shifts the graph to a nearly hyperbolic curve.

On the other hand, if the binding of the effector causes a conformational change that decreases the affinity of the enzyme for its substrate, the process is referred to as allosteric inhibition.

This decrease in enzyme function can result in a reduced rate of the chemical reaction compared to the activated state.

Key Terms and definitions

Allosteric Activation - The process by which a molecule binds to an enzyme to increase its activity.
Allosteric Enzymes - Enzymes that have multiple binding sites and are regulated by molecules that bind to these sites.
Allosteric Site - The place on an allosteric enzyme where a molecule that is not a substrate may bind, thus changing the shape of the enzyme and influencing its ability to be active.
Effector Molecules - Non-substrate molecules that bind to enzymes to regulate their activity. They can be either inhibitors or activators.
Covalent Modification - The process of an enzyme being activated or inhibited through the addition or removal of chemical groups.

Learning Objectives

Define Allosteric Activation - Understand the process by which a molecule activates an enzyme by binding to it (e.g., allosteric activation).
Contrast Allosteric Activation vs Inhibition - Know the difference between activators and inhibitors in the influence of enzyme functionality (e.g., activator vs inhibitor).
Explore Enzyme Regulation - Learn how the activities of enzymes are controlled to regulate metabolic pathways (e.g., effector molecules).
Explain the Allosteric Site - Understand the role of the allosteric site on enzyme activity and regulation.
Apply in Biochemical Context - Comprehend the significance of allosteric regulation in a biological context and how it affects the overall metabolic pathways.

Questions that this video will help you answer

[Question 1] What is allosteric activation and how does it influence enzyme functionality?
[Question 2] How does allosteric inhibition regulate enzyme activity?
[Question 3] What is the role of the allosteric site and effector molecules in enzyme regulation?

This video is also useful for

Students - Gain a profound understanding of how enzymes are regulated, a crucial concept in biochemistry and metabolic studies.
Educators - Provides a structured framework for teaching enzyme regulation, a foundational concept in biology and biochemistry courses.
Researchers - Useful for in-depth studies on enzymes and regulation mechanisms, critical for advancements in biomedical research and drug development.
Science Enthusiasts - Offers insights into the intricate workings of enzymes, sparking curiosity and enhancing knowledge in biology and life sciences.

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アロステリックレギュレーション