Question 1

What are the main differences between programmed and non-programmed cell death?

Accepted Answer

Programmed cell death occurs through regulated signals targeting specific cells, including apoptosis and autophagy. Non-programmed cell death, such as necrosis, happens accidentally due to infection, injury, toxins, or radiation. Necrotic cells swell and their membranes disintegrate, causing inflammation, whereas programmed death is controlled and orderly.

Question 2

How does apoptosis differ from autophagy in terms of cellular mechanisms?

Accepted Answer

Apoptosis involves caspase proteases degrading cellular proteins, causing cell shrinkage and apoptotic body formation. Autophagy accumulates damaged proteins and organelles in an autophagosome that fuses with a lysosome, where lysosomal enzymes degrade the contents. Both are programmed processes but operate through distinct molecular pathways.

Question 3

What happens to apoptotic bodies after they form during cell death?

Accepted Answer

After apoptotic bodies form during apoptosis, macrophages and surrounding cells remove them through phagocytosis of apoptotic cells. This process prevents inflammation and ensures clean removal of dead cell material. The phagocytic cells engulf and digest the apoptotic bodies, completing the orderly cell death process.

Question 4

Why is balance between cell proliferation and cell death important for health?

Accepted Answer

Balance between cell proliferation and death maintains tissue homeostasis. An imbalance can lead to cancer when cells proliferate excessively without adequate death, or autoimmune diseases when too many cells die. Proper regulation ensures the body maintains appropriate cell numbers and prevents disease development.

Question 5

What distinguishes necroptosis from other forms of programmed cell death?

Accepted Answer

Necroptosis combines features of apoptosis and necrosis. It begins when ligands bind death receptors, activating receptor-interacting protein kinases-1 instead of caspase-8. This triggers intermediate proteins causing cell swelling and lysis, creating a hybrid pathway between regulated apoptosis and accidental necrotic death.

Question 6

How does ferroptosis involve iron and reactive oxygen species in cell death?

Accepted Answer

Ferroptosis is an iron-dependent cell death process involving lipid peroxidation that accumulates reactive oxygen species. These reactive molecules eventually induce cell death. Morphologically, ferroptosis causes mitochondrial shrinkage and decreased cristae, distinguishing it from other programmed death pathways and contributing to diverse cellular outcomes.

Question 7

What role do caspases play in apoptosis and other cell death pathways?

Accepted Answer

Caspases are specific proteases that degrade cellular proteins during apoptosis, causing cell shrinkage and apoptotic body formation. Beyond apoptosis, caspase-1 and caspase-11 mediate pyroptosis, a caspase-dependent cell death required for inflammatory cytokine maturation. Caspases are central regulators in multiple programmed cell death pathways.

細胞死の概要