6.11
タンデム質量分析
Tandem mass spectrometry, or MS/MS, combines two mass analyzers in sequence, connected by a collision or interaction cell.
First, the samples are ionized to generate a mixture of ions, which enters the first mass analyzer.
This analyzer separates the ions and selects only a particular ion—the precursor ion—to move into the collision cell.
The collision cell contains inert gas molecules, which collide with precursor ions, fragmenting them into product ions.
These ions are run through the second mass analyzer, where they are separated and finally detected.
MS/MS generates various spectra.
Product-ion spectra are obtained when the first analyzer selects a precursor ion and the second analyzer scans all the masses.
Precursor-ion spectra are generated by scanning through precursor ions in the first analyzer and selecting a product-ion in the second analyzer.
To obtain neutral loss spectra, both mass analyzers are scanned together, with the second analyzer offset by the mass of a selected neutral fragment.
MS/MS is used for structural elucidation and complex mixture analysis.
タンデム質量分析法は、複数の質量分析装置を直列に使用して、分析物のより高い選択性を得て、分析物の検出時の化学ノイズを低減する技術です。相互作用セルによって分離された複数の分析装置を備えた機器により、二次フラグメンテーションとフラグメントイオンの選択された研究が可能になります。
二次断片化は相互作用細…
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