JoVE Journal > Neuroscience
Summary
Abstract
Protocol
Discussion
Disclosures
Acknowledgements
Materials
References
Neuroscience

昆虫に適応運動行動の神経基盤を研究し

Published: April 13, 2011
doi:
10.3791/2629
Please note that all translations are automatically generated. Click here for the English version.
, , ,
1Zoological Institute,University of Cologne

Summary

Abstract

Protocol

Discussion

Disclosures

The authors have nothing to disclose.

Acknowledgements

Materials

2-component epoxy glueUHU plus, UHU GmbH, Germany
glass screens (diameter 130mm)Linos Photonics
dental cementProTemp II, 3M ESPE, Seefeld, Germany3M Id : 70-2011-0358-0 Catalog Number : 46430Available through 3M (<a href="http://www.3m.com/">http://www.3m.com/</a>)or dental suppliers
fluorescent pigments Dr. Kremer Farbm&uuml;hle, Aichstetten, GermanyCat.#s: i.e 56200 Fluorescent Pigment Golden Yellow 56350 Fluorescent Pigment Flame Red<a href="http://kremer-pigmente.de/en">http://kremer-pigmente.de/en</a> or <a href="http://www.kremerpigments.com/">http://www.kremerpigments.com/</a>
histoacrylic glue 3Msupplier: WPI
coated copper wireElektrisola Eckernhagen<a href="http://www.elektrisola.com/">http://www.elektrisola.com/</a>
electrode cream Marquette Hellige, Freiburg, GermanyProduct is now discontinued, we suggest for example: <a href="http://www.grasstechnologies.com">www.grasstechnologies.com</a>
pulse generator Model MS501, electronics workshop, Zoological Institute, Cologne, Germany
lock-in-amplifier Zoological Institute
AD converter Cambridge Electronic Design
preamplifier Zoological Institute
high speed video camera Allied Vision Technologies
UV LED arrays Zoological Institute30-50V DC, electronics workshop<br/> λ390-395nm<br/>Luminance 24cd
Digitalizing software Spike2 Vers.5.05, CED, Cambridge, UK
motion tracking software Mikromak service, Berlin, GermanyWINanalyze, Vers.1.9
DOWNLOAD MATERIALS LIST

References

  1. Bässler, U., Wegener, U. Motor output of the denervated thoracic ventral nerve cord in the stick insect Carausius morosus. J. Exp. Biol. 105, 127-147 (1983).
  2. Böhm, H., Messai, E., Heinzel, H. -. G. Activity of command fibres in free-ranging crayfish, Orconectes limosus Raf. Naturwissenschaften. 84, 408-410 (1997).
  3. Büschges, A., Gruhn, M. Mechanosensory feedback in walking: from joint control to locomotor pattern. Adv.Insect Physiology. 34, 194-234 (2008).
  4. Büschges, A., Akay, T., JP, G. a. b. r. i. e. l., Schmidt, J. Organizing network action for locomotion: insights from studying insect walking. Brain Res Rev. 57, 162-171 (2008).
  5. Camhi, J. M., Levy, A. Organization of a complex movement: fixed and variable components of the cockroach escape behavior. J Comp Physiol A. 163, 317-328 (1988).
  6. Camhi, J. M., Nolen, T. G. Properties of the escape system of cockroaches during walking. J Comp Physiol A. 142, 339-346 (1981).
  7. Clarac, F., Libersat, F., Pflüger, H. J., Rathmayer, W. Motor pattern analysis in the shore crab (Carcinus maenas) walking freely in water and on land. J Exp Biol. 133, 395-414 (1987).
  8. Duch, C., Pflüger, H. J. Motor patterns for horizontal and upside down walking and vertical climbing in the locust. J Exp Biol. 198, 1963-1976 (1995).
  9. Fermi, G., Reichhardt, W. Optomotorische Reaktionen der Fliege Musca domestica. Abhängigkeit der Reaktion von der Wellenlänge, der Geschwindigkeit, dem Kontrast und der mittleren Leuchtdichte bewegter periodischer Muster. Kybernetik. 2, 15-28 (1963).
  10. Fischer, H., Kautz, H., Kutsch, W. A radiotelemetric 2-channel unit for transmission of muscle potentials during free flight of the desert locust, Schistocerca gregaria. J Neurosci Methods. 64, 39-45 (1996).
  11. Friza, F. Zur Frage der Färbung und Zeichnung des facettierten Insektenauges. Zeitschr. f. vergl. Physiologie. 8, 289-336 (1928).
  12. Götz, K. G. Principles of optomotor reactions in insects. Bibl. Ophthalmol. 82, 251-2559 (1972).
  13. Gruhn, M., Rathmayer, W. An implantable electrode design for both chronic in vivo nerve recording and axon stimulation in freely behaving crayfish. J Neurosci Methods. 118, 33-40 (2002).
  14. Gruhn, M., Hoffmann, O., Dübbert, M., Scharstein, H., Büschges, A. Tethered stick insect walking: a modified slippery surface setup with optomotor stimulation and electrical monitoring of tarsal contact. J Neurosci Methods. 158, 195-206 (2006).
  15. Gruhn, M., Zehl, L., Büschges, A. Straight walking and turning on a slippery surface. J Exp Biol. 212, 194-209 .
  16. Gruhn, M., Uckermann, G. v. o. n., Westmark, S., Wosnitza, A., Büschges, A., Borgmann, A. Control of stepping velocity in the stick insect Carausius morosus. J Neurophysiol. 102, 1180-1192 .
  17. Hama, N., Tsuchida, Y., Takahata, M. Behavioral context-dependent modulation of descending statocyst pathways during free walking, as revealed by optical telemetry in crayfish. J Exp Biol. 210, 2199-2211 (2007).
  18. Hassenstein, B. Über die Wahrnehmung der Bewegung von Figuren und unregelmäβigen Helligkeitsmustern (nach verhaltensphysiologischen Untersuchungen an dem Rüsselkäfer Chlorophanus viridis. Zeitschr. f. vergl. Physiologie. 40, 556-592 (1958).
  19. Jander, J. P. . Untersuchungen zum Mechanismus und zur zentralnervösen Steuerung des Kurvenlaufs bei Stabheuschrecken (Carausius morosus) [dissertation]. , (1982).
  20. Jander, R., Volk-Henrichs, I. Das strauch-spezifische Perzeptorsystem der Stabheuschrecke (Carausius morosus). Zeitschr. f. vergl. Physiologie. 70, 425-447 (1970).
  21. Kirschfeld, K., Zettler, F., Weiler, R. The resolution of lens and compound eyes. Neural Principles in Vision. , 354-370 (1976).
  22. Kutsch, W., Schwarz, G., Fischer, H., Kautz, H. Wireless transmission of muscle potentials during free flight of a locust. J Exp Biol. 185, 367-373 (1993).
  23. Scharstein, H. A universal projector for optomotor stimulation. , (1985).
  24. Schlegtendal, A. Beitrag zum Farbensinn der Arthropoden. Zeitschr. f. vergl. Physiologie. 20, 573-581 .
  25. Schmidt, J., Grund, M. Rhythmic activity in a motor axon induced by axotomy. Neuroreport. 14, 1267-1271 (2003).
  26. Shankar, P. N., Kumar, M. Experimental Determination of the Kinematic Viscosity of Glycerol-Water Mixtures. Proc. Mathematical and Physical Sci. 444, 573-581 (1994).
  27. Tryba, A. K., Ritzmann, R. E. Multi-Joint coordination during walking and foothold searching in the Blaberus cockroach I. Kinematics and electromyograms. J Neurophysiol. 83, 3323-3336 .
  28. Tryba, A. K., Ritzmann, R. E. Multi-Joint coordination during walking and foothold searching in the Blaberus cockroach. II. Extensor motor neuron pattern. J Neurophysiol. 83, 3337-3350 .
  29. Tsuchida, Y., Hama, N., Takahata, M. An optical telemetry system for underwater recording of electromyogram and neuronal activity from non-tethered crayfish. J Neurosci Methods. 137, 103-109 (2004).
  30. Wang, H., Ando, N., Kanzaki, R. Active control of free flight maneuvers in a hawkmoth, Agrius convolvuli. J Exp Biol. 211, 423-432 (2008).

Tags

Neural BasisAdaptive Locomotor BehaviorInsectsWalking BehaviorNeuronally Produced Stepping OutputPassive ForcesInteractions With Other LegsKinematic AnalysisElectrophysiological RecordingsMotor ActivityRecording Of Neuronal Or Muscular ActivityElectrophysiological EquipmentImplantable ElectrodesLong TetherTelemetric DevicesArthropodsSmaller Walking Insects
Studying the Neural Basis of Adaptive Locomotor Behavior in Insects

Play Video
PDF
DOI
DOWNLOAD MATERIALS LIST
Cite This Article
Gruhn, M., Rosenbaum, P., Bollhagen, H., Bueschges, A. Studying the Neural Basis of Adaptive Locomotor Behavior in Insects. J. Vis. Exp. (50), e2629, doi:10.3791/2629 (2011).
Download Citation
Reprints and Permissions

View Video

✖

To prove you're not a robot, please enter the text in the image below

CAPTCHA Image
Play CAPTCHA Audio
Refresh Image