Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

サマリー

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

要約

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

プロトコル

腎組織開発のための上皮細胞の再定着およびげっ歯類細胞外マトリックススキャフォールドの調製に関して正誤表が発行されました。

本研究およびCaraltらの研究で使用したヒトRCTEC/RCTE細胞ストック 他、 2015年, Uzarski ら、 2015年に、2001年のMTAを通じて、共著者のDr. Wandinger-Ness（ニューメキシコ大学）にDr. Loghman-Adham（当時セントルイス大学）より提供されたものである。SV40不死化ヒトRCTEC/RCTE細胞は、以下の詳細に記載されている通り、遠位尿細管細胞株として特性解析された。 ロクマン・アダム 他、 2003年、RCTEC/RCTE細胞ストックの初期継代分に対して最近実施されたショートタンデムリピート（STR）DNAシーケンシング（IDEXX BioResearch社により実施）に基づき、これらの細胞が混合系統であることが明らかになった。QIAxcelキャピラリー電気泳動を用いたPCR産物のさらなる解析により、イヌ由来産物の存在が示された。この産物をシーケンシングした結果、イヌ由来であることが確認された。また、一連のイヌ特異的STRマーカーをサンプルと比較したところ、当該サンプルの遺伝的プロファイルはMDCK細胞株と92%の同一性を示した。  本論文で使用されたその後の細胞ストックについてSTRプロファイリングを行ったところ、100% MDCK系統へのドリフトが認められました。MDCKは、以前考えられていたヒト由来ではなくイヌ由来ですが、同様に遠位尿細管上皮細胞株です。このため、本研究および〜においてこれらの細胞を用いて導き出された以前の解釈および結論は、 Caralt 他、 2015, Uzarski 他、 2015年の結果は依然として妥当ですが、厳格性、再現性、および他者による実験的検証を目的として、この誤同定について報告します。この情報は、International Cell Line Authentication Committee (ICLAC) のデータベース (http://iclac.org) への登録のためにも報告されます。