内脂肪量の生化学的および高スループット微視的評価<em>線虫（Caenorhabditis elegans）</em
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March 30, 2013
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私たちは、勉強のための堅牢な生化学的および顕微鏡的手法を提示<em>線虫（Caenorhabditis elegans）</em>脂質店。蛍光脂肪滴のイメージングのための迅速、シンプル、固定·染色手順は、親油性染料ナイルレッドのスペクトル特性を活用しています。次に、トリグリセリドおよび固相抽出とガスクロマトグラフィー - 質量分析法を用いたリン脂質の生化学的測定を提示します。
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Pino, E. C., Webster, C. M., Carr, C. E., Soukas, A. A. Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans. J. Vis. Exp. (73), e50180, doi:10.3791/50180 (2013).
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