Microfluidic Chip Fabrication and Method to Detect Influenza

The overall goal of the following experiment is to detect influenza A virus using a microfluidic chip. First, run the sample with lysis buffer through the SPE channel to lyce the virus and extract RNA load the R-T-P-C-R reagent with extracted RNA and run the reverse transcription channel to convert viral RNA into CDNA. Next, run the mixture into the PCR channel in order to amplify the DNA results are obtained that show the size and concentration of the amplicon based on the fluorescence signal from capillary electrophoresis.

The main advantage of this technology is it accepts the raw patient sample and it integrates nucleic acid extraction, purification, reverse transcription and amplification into a single chip To create two plaques, distribute approximately nine grams of XN X pellets evenly in the center of a metal plate, place on a 198 degrees Celsius heated press for five minutes. Then apply pressure slowly to 2, 500 PSI for another five minutes. Now, position one plaque onto the epoxy mold preheat at 157 degrees Celsius for 10 minutes, and apply pressure slowly to 1000 PSI for another 10 minutes.