13,088 Views
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11:06 min
February 24, 2014
DOI:
10.3791/50752-v
ここでは、LacIリトランスジェニックマウスモデルを用いて精製した造血細胞の数が少ないため、生体内突然変異誘発アッセイを説明します。 lacI遺伝子は、DNA突然変異の頻度、場所、タイプ、自然に生じまたは遺伝毒性物質への曝露後を決定するために単離することができる。
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Cite this Article
Cheng, Z., Zhou, T., Merchant, A., Prihoda, T. J., Wickes, B. L., Xu, G., Walter, C. A., Rebel, V. I. Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells. J. Vis. Exp. (84), e50752, doi:10.3791/50752 (2014).
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