ジャーナル
/
免疫学と感染
/
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

17,295 Views

•

14:14 min

•

January 06, 2014

DOI:

10.3791/50823-v

DOI:
10.3791/50823-v

•

14:14 min
January 06, 2014

•

8 Views
, , ,
1小児科,Harvard Medical School, 2Department of Pediatric Gastroenterology,MGH for Children, 3Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

概要

Automatically generated

Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.

Explore More Videos

In Vitro CocultureNeutrophil Trans-epithelial MigrationMucosal ImmunityPathogenic InfectionPolarized Epithelial MonolayerNeutrophil InfiltrationInflammatory ResponseTherapeutic Targets

Read Article

Cite this Article

Kusek, M. E., Pazos, M. A., Pirzai, W., Hurley, B. P. In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration. J. Vis. Exp. (83), e50823, doi:10.3791/50823 (2014).

Download .ris file

Copy

Share Video

.

Copy