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12:48 min
March 25, 2014
DOI:
10.3791/51418-v
ショウジョウバエの組織は、しばしば、細胞型の不均一な混合物を含有する。特定の組織の特定の細胞型における遺伝子発現を調べるために、核が遺伝的にタグ付けし、続いて親和性に基づくアプローチを用いて単離することができる。単離された核は、遺伝子発現解析およびクロマチン免疫沈降などの下流用途に使用することができる。
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Ma, J., Weake, V. M. Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis. J. Vis. Exp. (85), e51418, doi:10.3791/51418 (2014).
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