Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Krzysztof Bobik; John R. Dunlap; Tessa M. Burch-Smith

doi:10.3791/51844

ジャーナル

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生物学

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タンデム高圧凍結および透過型電子顕微鏡のための植物組織の急速凍結置換

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

JoVE Journal
生物学

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JoVE Journal 生物学

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

タンデム高圧凍結および透過型電子顕微鏡のための植物組織の急速凍結置換

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16,341 Views

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12:52 min

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October 13, 2014

DOI:

10.3791/51844-v

DOI:

10.3791/51844-v

•

12:52 min

October 13, 2014

•

2 Views

Krzysztof Bobik¹, John R. Dunlap², Tessa M. Burch-Smith¹

¹Department of Biochemical, Cellular and Molecular Biology,University of Tennessee, Knoxville, ²Advanced Microscopy and Imaging Facility,University of Tennessee, Knoxville

概要

Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.